葡萄糖氧化酶产生菌的快速筛选及其产酶条件的研究

通过平板快速筛选方法从土壤样品中分离得到一株产葡萄糖氧化酶菌株,此菌株属于产胞内酶的黑曲霉。采用文献报道的相关工艺测得其初始酶活为0.86μmol/min。经过培养基配方的优化试验及摇床发酵工艺的调整,其摇床发酵的产酶率有了较大的提高,发酵液过滤液酶活达到9.91μmol/min。     

美国黑豚养殖技术

美国黑豚属野生皮毛兽种类,是食草哺乳动物,形态小而肥胖,通身黑色与兔十分相似,成龄黑豚体重一般在800g左右,最重可达到1500-2000g,前肢短小,不善跳跃,行动敏捷。皮毛丰厚细密、黑亮光涌,是加工高档裘皮服装和工艺饰品的加工原料,豚肉是味道鲜美的上等佳品,其血液是提取歧氧化酶的最理想原料。具有很高的经济值和利用价值。     

铬对畜禽机体免疫功能的影响

<正>自1749年铬(Chromium)被法国化学家Louis Vauguelin发现后,在很长的一段时间内,人们认为铬是一种有毒有害的矿物元素。直到1954年,Curan发现在大鼠的日粮中添加铬后,能够使肝脏合成胆固醇和脂     

定性检测饲料中三聚氰胺便捷方法的探讨

本实验在蛋白质饲料样品中按照梯度添加三聚氰胺,然后用热蒸馏水和三氯乙酸溶液处理,将上清液离心后滴入三聚氰酸饱和溶液,三聚氰胺与三聚氰酸在水中结合成异氰尿酸蜜胺盐,异氰尿酸蜜胺盐不溶于水,是白色沉淀物,形成乳浊液。根据反应的现象可以定性地判断饲料样中是否含有三聚氰胺。结果表明:当鱼粉和豆粕中三聚氰胺含量分别高于60mg/kg和70mg/kg时,可用此方法快捷、简便的判断样品中含三氯氰胺。     

浅谈动物尸体处理现状与危害及对策

随意抛弃动物尸体,具有潜在的疾病传播危险,一旦将携带人畜共患病病原体的动物尸体抛弃在垃圾桶、河道甚至居民区,将会严重危害人类的健康,即便是正常死亡的动物尸体,也会对环境造成污染。现就动物尸体处理现状、危害及对策浅谈如下,以供同行商榷。     

辽育白牛两部位牛肉营养成分和氨基酸组成的分析

对6头22～24月龄辽育白牛育肥牛的眼肉和腹肉的常规营养成分、氨基酸组成测定分析,结果表明:辽育白牛眼肉和腹肉的水分、蛋白质、脂肪、灰分含量分别为59.5%±3.02%、18.17%±1.22%、24.03%±4.48%、0.84%±0.09%和58.69%±1.21%、15.84%±1.22%、34.18%±7.01%、0.74%±0.04%;必需氨基酸、总氨基酸、必需/总氨基酸、必需/非必需氨基酸含量分别为6.04 g/100 g±0.15 g/100 g、16.4 g/100 g±0.19 g/100 g、36.83%±0.17%、58.3%±0.26%和6.59 g/100 g±0.18 g/100 g、17.72 g/100 g±0.18 g/100 g、37.19%±0.19%、59.21%±0.31%,蛋白质中必需氨基酸组成比例基本符合FAO/WHO的标准。因此,辽育白牛眼肉和腹肉主要营养成分和氨基酸含量构成较为理想,适合生产高档牛肉。     

镇海巨高长毛兔体重与产毛性能相关的研究

利用原全国家兔育种委员会2000年长毛兔性能测定数据,分析研究了镇海巨高长毛兔12月龄时体重与产毛性能的相关。结果表明:母兔的体重、标准含水率产毛量、单次产毛率和估测年产毛率均显著或极显著地高于公兔;随着体重的增加,标准含水率产毛量相应地提高,单次产毛率和估测年产毛率相应地下降;体重与标准含水率产毛量、单次产毛率的相关系数分别为0.225、-0.324(P<0.01);标准含水率产毛量与单次产毛率的相关系数为0.845(P<0.01)。     

我国出口观赏鸟血液原虫的调查与分析

通过显微镜检查对来源于全国各地的37种出口观赏鸟进行了血液原虫调查。共检查血样1144份，结果为：血液原虫的检出率为2．1％，主要是血变原虫和／或疟原虫。感染季节以秋冬两季为主，易感鸟品种主要是白腰文鸟、红喉歌鸲、蓝歌鸲、暗绿绣眼鸟、豆冠、白燕、情侣鹦鹉。     

Reducing methane emissions and the methanogen population in the rumen of Tibetan sheep by dietary supplementation with coconut oil

The objective was to evaluate the effect of dietary coconut oil on methane (CH(4)) emissions and the microbial community in Tibetan sheep. Twelve animals were assigned to receive either a control diet (oaten hay) or a mixture diet containing concentrate (maize meal), in which coconut oil was supplemented at 12?g/day or not for a period of 4?weeks. CH(4) emissions were measured by using the 'tunnel' technique, and microbial communities were examined using quantitative real-time PCR. Daily CH(4) production for the control and forage-to-concentrate ratio of 6:4 was 17.8 and 15.3?g, respectively. Coconut oil was particularly effective at reducing CH(4) emissions from Tibetan sheep. The inclusion of coconut oil for the control decreased CH(4) production (in grams per day) by 61.2%. In addition, there was a positive correlation between the number of methanogens and the daily CH(4) production (R?=?0.95, P?相似文献   

Protective influence of rosiglitazone against time‐dependent deterioration of boar spermatozoa preserved at 17°C

Spermatozoa are highly specialized cells, and energy metabolism plays an important role in modulating sperm viability and function. Rosiglitazone is an antidiabetic drug in the thiazolidinedione class that regulates metabolic flexibility and glucose uptake in various cell types, but its effects on boar sperm metabolism are unknown. In this study, we investigated the potential effect of rosiglitazone against time‐dependent deterioration of boar spermatozoa during liquid preservation at 17°C. Freshly ejaculated semen was diluted with Beltsville Thawing Solution (BTS) containing different concentrations of rosiglitazone, and the motility, membrane and acrosome integrity of sperm were detected. Besides, we measured glucose uptake capacity, l ‐lactate production level, mitochondrial membrane potential, adenosine triphosphate (ATP) content and mitochondrial reactive oxygen species (mROS) production of sperm after boar semen had been incubated with or without rosiglitazone, iodoacetate (glycolysis inhibitor) and rotenone (electron transport chain inhibitor) for 5 days. The addition of rosiglitazone significantly enhanced sperm quality and had a strong protective effect on the sperm membrane and acrosome integrity during storage. BTS containing 50 μM rosiglitazone maintained the total motility of liquid‐preserved sperm above 60% for 7 days. Rosiglitazone improved sperm quality by regulating energy metabolism manner of preserved sperm, protected the sperm mitochondrial membrane potential, enhanced sperm ATP production and in the meanwhile reduced mROS through enhancing glycolysis but not oxidative phosphorylation. The data suggested the practical feasibility of using rosiglitazone for improving boar spermatozoa quality during semen preservation.