Comparative analysis of proteomic profiles between endometrial caruncular and intercaruncular areas in ewes during the peri-implantation period

The endometrium of sheep consists of plenty of raised aglandular areas called caruncular (C), and intensely glandular intercaruncular areas (IC). In order to better understand the endometrium involved mechanisms of implantation, we used LC-MS/MS technique to profile the proteome of ovine endometrial C areas and IC areas separately during the peri-implantation period, and then compared the proteomic profiles between these two areas. We successfully detected 1740 and 1813 proteins in C areas and IC areas respectively. By comparing the proteome of these two areas, we found 170 differentially expressed proteins (DEPs) (P < 0.05), functional bioinformatics analysis showed these DEPs were mainly involved in growth and remodeling of endometrial tissue, cell adhesion and protein transport, and so on. Our study, for the first time, provided a proteomic reference for elucidating the differences between C and IC areas, as an integrated function unit respectively, during the peri-implantation period. The results could help us to better understand the implantation in the ewes. In addition, we established a relatively detailed protein database of ovine endometrium, which provide a unique reference for further studies.     

犬的常见心理在警犬训练中的作用

犬的常见心理包括怀旧、好奇、复仇、恐惧和孤独等心理,把握好犬的这些心理特征,将会有利于警犬与人依恋性以及亲和关系的建立,有利于警犬衔取兴奋性的培养,可以增强警犬的凶猛性,能够更加有效地促进警犬对执勤环境的适应.     

The stimulatory effect of insulin-like growth factor-1 on the proliferation, differentiation, and mineralisation of osteoblastic cells from Holstein cattle

This study investigated the effect of exogenous insulin-like growth factor (IGF)-1 on the proliferation and differentiation of osteoblastic cells from Chinese Holstein cattle and the resultant bone nodule formation and mineralisation in vitro. The osteoblastic cells were isolated and cultured, then identified using Giemsa and alkaline phosphatase (ALP) staining methods. The effect of different concentrations of IGF-1 on cell growth was assessed by MTT assay. The ALP activity and osteocalcin (OC) concentration in the osteoblastic cells were measured by a colorimetric assay and a radioimmmunoassay, respectively. Calcium nodules were observed using alizarin red S stain, while the content of matrix calcium was determined by atomic absorption spectrophotometry. Cell proliferation in the cultures was stimulated by IGF-1 at concentrations ranging from 1 to 200 ng/mL, with the maximum effect observed at 100 ng/mL. This effect was observed from day 1 and peaked at day 5, but decreased at day 7. At concentrations of 10 ng/mL and 100 ng/mL, IGF-1 significantly induced ALP activity, OC level, matrix calcium content, and nodule formation of the osteoblastic cells by 20–180% (P < 0.05 or P < 0.01), compared to controls. The results suggested that IGF-1 is an anabolic agent for the proliferation, differentiation, mineralisation and calcium content of dairy cow osteoblasts, and could therefore act as a potential treatment for the metabolic bone diseases in these animals.     

PKS-NRPS数据库的研究进展

非核糖体多肽合成酶(NRPS)和聚酮合成酶(PKS)是调控次级代谢物聚酮类物质和非核糖体多肽合成的关键酶。随着生物息学的快速发展,国外的NRPS-PKS数据库和预测软件不断推出。本文对几个重要NRPS和PKS领域的数据库与预测软件的研究做一综述     

陆地棉转录因子GhNAC78基因的特征及功能分析

本实验室从陆地棉中克隆得到GhNAC78,分析了其基因特征及功能。该基因cDNA全长为937 bp,开放阅读框为876bp,编码291个氨基酸,隶属于NAM转录因子亚家族。体外转录激活实验表明,GhNAC78具有转录激活活性。上游启动子区1537 bp序列的生物信息学分析可知其包含多种激素、胁迫和光响应元件,表明GhNAC78广泛地参与植物生长发育和胁迫响应的调控。荧光定量结果显示,GhNAC78在棉花叶片衰老过程中高调表达,推测其参与叶片衰老的调控。     

花粉管通道法介导的棉花遗传转化

花粉管通道法介导的遗传转化是一种借助于植物自身的种质细胞卵细胞或受精卵为转化对象的直接转化技术。作为一种颇具特色的转基因技术,由于具备无基因型限制和易于实现大规模基因转化的特点,可以在任何开花植物和不同物种之间实现基因的转移,使得受体物种在获得新的基因型的同时,也获得转基因所表达的新的表型性状,由此成为为农作物转基因育种的崭新技术途径。利用花粉管通道法,成功地进行了棉花的抗虫／抗病的基因工程,获得了多种实用化的抗虫／抗病转基因棉花新品种(系),进入了棉花大面积生产。     

烤烟叶片发生、成熟与有效积温的关联分析

为筛选出烤烟叶片发生、成熟与有效积温的关系,采用盆栽试验研究了‘红花大金元’和‘K326’不同移栽时间和不同施肥条件下第4、10、16叶位的烟叶发生、成熟与有效积温的关系,分析对烟株生长和烟叶产质量的影响。结果表明,不同移栽时间的第4叶位成熟所需有效积温为304.9~370.6℃,第10叶位成熟所需有效积温为569~706℃,第16叶位成熟所需有效积温为758.3~812℃。综合分析说明,相同叶位烟叶成熟时其有效积温的积累量是相对稳定的区段范围,可以通过大田试验验证利用不同叶位发生、成熟与有效积温的关系指导生产的可行性。     

"太行山道路"与大学精神

通过对太行山道路的实践总结,阐述了太行山道路与太行山精神形成的历史背景和深刻内涵。指出太行山精神的实质是体现河北农业大学办学特色和彰显河北农业大学个性的大学精神。坚持太行山道路与保持办学特色、增强核心竞争力是内在统一的。提出应继续弘扬太行山精神,不断强化学校独特的精神品格。     

巨尾桉树干(去皮)含水率与生物量的研究

研究巨尾桉树干 (去皮 )含水率的分布规律后 ,认为巨尾桉树干 (去皮 )的含水率可以采用调查 3/1 0 H处的含水率获得 ,大大减小巨尾桉树干 (去皮 )生物量调查工作量 .选用模型W =0 .0 1 1 2 70 7( D2 H ) 1.0 3 919,R =0 .9863编制了巨尾桉二元树干 (去皮 )绝干重表 ,并在此基础上导出巨尾桉一元树干 (去皮 )绝干重表     

陕西地区苹果金纹细蛾成虫消长规律的观察

<正>金纹细蛾Lithocolletis ringoniella别名苹果细蛾,属鳞翅目细蛾科,是苹果树上一种重要潜叶害虫,也可为害梨、桃、李、樱桃、海棠等果树,分布于我国华北、辽宁、山东、河南、江苏、安徽、陕西、甘肃及日本和欧美国家等。20世纪90年代开始,由于苹果栽培品种、方式、结构等的影响,该虫从次要害虫上升为主要害虫(文耀东,2004)。目前对该虫生物学特性、发生及分布规律、防治方法等内容研究较多(曹春玲等,2014),但对其成虫在苹果园的分布及活动