雏鸭饲粮锰适宜水平的研究

采用玉米-豆饼型饲粮(含Mn19ppm),对210只1日龄北京雏鸭进行补锰试验,试验分7组,锰添加水平(试剂级MnSO_4·H_2O)按1～7组顺序依次为0,20,45,60,85,110,135ppm,试验期3周,研究雏鸭饲粮锰适宜水平。结果为,饲粮锰水平不影响雏鸭体增重,日采食量和饲料转化率(P>0.05);胫骨锰浓度随饲粮锰水平增加呈线性上升(P<0.01);肝、肾、胰、心组织锰浓度和肝脏含锰的SOD酶活性可与饲粮锰水平拟合为相应的二次曲线模式(P<0.001),上述软组织锰浓度和肝SOD酶活性达到平衡时,所需饲粮锰依次为110.05,100.00,122.91,113.99和116.00ppm。综合试验结果考虑,雏鸭饲粮锰适宜水平为110ppm。     

4株酸奶噬菌体的分离

从上海光明乳业股份有限公司车间发酵罐中分离出4株酸奶噬菌体。1号噬菌体头部呈六角形，平均直径为95.1nm，头部剖面平均面积为8184．8nm^2，尾部的平均直径为18．3nm，平均长度为403．4nm；2号噬菌体头部呈六角形，平均直径为85．4nm，头部剖面平均面积为8046．2nm^2，尾部的平均直径为23．3nm，平均长度为549．4nm；3号噬菌体头部呈六角形，平均直径为95．5nm，头部剖面平均面积为9792．3nm^2，尾部的平均直径为19．8nm，平均长度为450．5nm；4号噬菌体头部呈六角形，平均直径为119．3nm．头部剖面平均面积为12586．1nm^2，尾部的平均直径为23．6nm，平均长度为520．5nm。     

品种及初生重对仔猪死亡率和21日龄个体重的影响

试验对2 319头仔猪的初生重与21日龄个体重和死亡率进行分析。结果表明:大约克仔猪哺乳期死亡率最低,21日龄体重最高;随着初生重的增加,仔猪死亡率降低,21日龄个体重增加。     

15%毒.高氯乳油的液相色谱分析

本法采用高效液相色谱法对毒死蜱和高效氯氰菊酯混合制剂作定量分析。变异系数分别为０．８４％,１．２０％,标准偏差分别为０．１１３６,０．０１８５;回收率分别为９７．７９％－１００．１５％,９７．４７％－９９．３６％。     

雏鸭病毒性肝炎弱毒疫苗的研究——Ⅰ.弱毒疫苗的培育及实验室试验

水试验通过将鸭肝炎病毒(DNV)在鸡胚传代,培育出雏鸭用鸭病毒性肝炎(DVH)弱毒疫苗——鸭病毒性肝炎81代鸡胚化弱毒疫苗(简称BAU-1),并对该苗进行了实验室试验。 试验表明,BAU-1对雏鸭安全无毒性,稳定性好,在敏感鸭体内连传5代无毒力返强现象。经测定,BAU-1的免疫剂量为5×10~(9·4)ELD50/只(皮下免疫)。通过对免疫雏鸭的血清抗体滴度进行测定,证实该BAU-1疫苗对雏鸭的免疫保护期在6周以上。     

天麻田间高产栽培措施试验

为提高天麻栽培产量，扩大天麻栽培地域，提高山区有限土地利用率，笔者将影响天麻田间高产栽培的几个关键因子进行比较试验，总结出天麻田间高产栽培的有效措施，为大力发展天麻生产提供了科学依据。     

苯并芘对猪卵母细胞SHH信号通路影响的研究

如今空气污染问题仍广泛存在,其中苯并芘（Benzo （a） pyrene,BaP）是一种常见的空气污染物,对人和动物的健康具有严重影响。据报道,BaP可以显著降低卵母细胞IVM （卵母细胞体外成熟）能力,但机制尚不清楚。因此,本研究在猪卵母细胞IVM培养液中添加不同浓度BaP （50 μM、100 μM、200 μM）检测其对卵母细胞IVM能力的影响,并检测此过程SHH信号通路相关基因和蛋白的表达。结果显示50 μM BaP处理组卵母细胞IVM能力显著低于对照组（76.5 vs 68.1,P<0.05）,并随着BaP浓度增加进一步下降。同时,qPCR和免疫荧光染色检测发现,50 μM BaP处理组卵母细胞中SHH信号通路相关基因mRNA和蛋白表达量,均显著低于对照组（P<0.05）。因此,本研究初步证明BaP可能通过破坏SHH信号通路降低猪卵母细胞IVM能力。     

The Fluorescent Characterization and Analysis of Two Brucella Attenuated Vaccine Infecting Mouse Macrophagocyte

The experiment was conducted to discuss the difference of binding time of green fluorescent protein B.melitensis M5 (GFP-M5) and B.abortus S19 (GFP-S19) infecting the mouse macrophagocyte (RAW264.7),lysosome,endoplasmic reticulum and golgi body in the initial stage and compare the binding rate of GFP-M5,GFP-S19 with organelle in different timeline,respectively,by confocal laser scanning microscope (CLSM) and flow cytometry.The result showed that GFP-M5 and GFP-S19 were successfully constructed.The intracellular survival ability of Brucella M5,Brucella S19,GFP-M5 and GFP-S19 were not obvisouly affected after infecting RAW264.7.GFP-M5 and GFP-S19 could enter the macrophagocyte in 30 mins,and in 2 h the Brucella could reach lysosome,endoplasmic reticulum and golgi body.In addition,the binding time for two attenuated vaccine did not show differences in 1,2,3 and 4 h.The content of GFP⁺ cell produced by RAW264.7 infected by GFP-M5 and GFP-S19 did not show significant differences (P>0.05).Therefore,the two strains did not have significant differences in the invasion ability in the initial stage of infecting host cell.     

姜田土壤特性对南方根结线虫密度的影响

对山东省姜田土壤特性与南方根结线虫密度进行了逐步回归和通径分析 ,同时进行了曲线拟合处理。表明 :土壤总空隙度 37.91%、有机质含量 3.16 %、有效磷含量 130 .6 9× 10 - 6 、有效铁含量 14 5 .93× 10 - 6 和有效锰含量 4 8.6 0× 10 - 6 时南方根结线虫密度最低 ,有效钾含量在 2 4 3.6 2× 10 - 6 时南方根结线虫密度最高。提出适当降低土壤总空隙度和有效钾含量 ,尽可能提高土壤中有机质、有效磷、有效铁和锰含量的土壤改良方案。     

Generation of a uniform thymic malignant lymphoma model with C57BL/6J p53 gene deficient mice

Lymphoma is the third most common cancer diagnosed in children, and T-cell lymphoma has the worst prognosis based on clinical observations. To date, a lymphoma model with uniform penetrance has not yet been developed. In this study, we generated a p53 deficient mouse model by targeting embryonic stem cells derived from a C57BL/6J mouse strain. Homozygous p53 deficient mice exhibited a higher rate of spontaneous tumorigenesis, with a high spontaneous occurrence rate (93.3%) of malignant lymphoma. Because tumor models with high phenotypic consistency are currently needed, we generated a lymphoma model by a single intraperitoneal injection of 37.5 or 75 mg/kg N-methyl-N-nitrosourea to p53 deficient mice. Lymphoma and retinal degeneration occurred in 100% of p53^+/− mice administered with higher concentrations of N-methyl-N-nitrosourea, a much greater response than those of previously reported models. The main anatomic sites of lymphoma were the thymus, spleen, bone marrow, and lymph nodes. Both induced and spontaneous lymphomas in the thymus and spleen stained positive for CD3 antigen, and flow cytometry detected positive CD4 and/or CD8 cells. Based on our observations and previous data, we hypothesize that mice with a B6 background are prone to lymphomagenesis.