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51.
The present study was carried out to identify the excretory/secretory (E/S) antigens of the rumen infecting digenetic trematode Gastrothylax crumenifer that may be useful for the immunodiagnosis of rumen amphistomosis particularly during the pre-monsoon season during which this rumen parasite stops shedding eggs. The in vitro released E/S proteins were purified on a Sephadex G-200 column. The gel filtration profile revealed three distinct fractions F1-F3 where F1 and F3 appeared as sharp peaks while the F2 fraction was dispersed. The antibody titre against each of the purified E/S fractions was determined by ELISA using anti-whole E/S polyclonal antibodies raised in rabbit. Among the three fractions, the antibody titre against F1 was highest (1:12,800) whereas IgG titre was very low (1:50) for fraction F2 and F3 (1:100). Of the total polypeptides resolved on gradient SDS-PAGE, only a few antigenic polypeptides were detected in each fraction with hyperimmune anti-serum as revealed by Western Blot analysis. However, a 33 kDa antigen detected in each fraction appeared to be immunodominant which could be exploited for the diagnosis of the pouched amphistome. 相似文献
52.
The roots of 20 days old seedlings of tomato (Lycopersicon esculentum Mill.) at the time of their transplantation, were dipped in 10−8, 10−7, 10−6 or 10−5 M of 28-homobrassinolide (HBR) for 15, 30 or 45 min and were allowed to grow in earthen pots, in a net house. The leaves of the plants, at days 30 and 60, possessed elevated quantities of nitrate reductase (NR), carbonic anhydrase (CA) and the contents of chlorophyll. The values for all the above characteristics were significantly higher than that of the water-fed control. The fruits borned at the treated plants were more in number and possessed a lower quantity of ascorbic acid than the control. Moreover, the fruits at ripening, had higher levels of lycopene and β-carotene. Among the treatments, 15 min feeding of 10−8 M HBR proved best. 相似文献
53.
Shahzad Ali Amanullah Jan Jia Zhikuan Amir Sohail Cai Tie Wei Ting Zhang Peng Manzoor Irshad Ahmad Mati Ur Rahman Ren Xiaolong Liu Xiaoli Xu Yue Yue 《Russian Agricultural Sciences》2016,42(3-4):224-229
A field experiment was conducted on silty clay loam soil in the years 2011–2012. Two sesame (Sesamum indicum L.) cultivars (Local Black and Local White) were evaluated using various 3 different sowing dates (20th June, 10th and 30th July) and four agrotechnical level (0, 40, 80 and 120 kg N ha–1) at New Developmental Farm The University of Agriculture, Peshawar, Pakistan. The objective of this study was to evaluate the effects of sowing dates on growth, yield and oil fatty acid composition of two sesame cultivars grown under different nitrogen fertilization. Results showed that cv. Local Black was characterized by significantly higher content of oil (47%), seed yield (696 kg ha–1) and oil yield (335 kg ha–1) while cv. Local White had higher palmitic acid (8%) and linoleic acid (38.7%). Yield and its main components were positively affected by the earlier sowing date. With regard to fatty acid composition, a decrease in oleic and stearic acid and an increase in linoleic and palmitic acid were observed. At early sowing, oleic and palmitic acid decreased whereas linoleic and stearic acid increased. The decrease in the oleic/linoleic acid ratio observed at early sowing, suggests a possible role of temperature on the activity of oleate desaturase in the developing seeds. Intensive technology of cultivation (120 kg N ha–1), compared to the economical technology (40 kg N ha–1), significantly increased the seed yield of both sesame cultivars. This was due to higher number of branches, Capsules m–2, capsules plant–1, seeds capsule–1 and 1000 seed weight (g). The intensive technology of cultivation had a beneficial effect on the content of palmitic acid, linolenic acid and oleic acid in sesame seed. 相似文献
54.
The isolation and NMR spectra of otosenine (1) and seneciphylline (2) from Senecio lorenthii are reported. 相似文献
55.
Sahab Hedjazi Othar Kordsachia Rudolf Patt Ahmad Jahan Latibari Ulrike Tschirner 《Industrial Crops and Products》2009,29(1):27-36
Alkaline sulfite–anthraquinone (AS/AQ) pulping of wheat straw and totally chlorine free (TCF) bleaching of resulting pulps was investigated. Wheat straw was supplied by the Ferdosii University Experimental Station in Northeastern Iran, cleaned and chopped into about 3–5 cm long pieces. In the AS/AQ pulping experiments, the active alkali charge on oven dry wheat straw, heating time to maximum cooking temperature, AQ dose and liquor to straw ratio were kept constant at 16%, 60 min, 0.1% and 4/1, respectively. The alkali ratio, Na2SO3/NaOH, calculated as NaOH, was varied from 20/80 to 80/20. Cooking time at maximum temperature was varied from 30 to 90 min and maximum cooking temperature was adjusted between 152 and 160 °C. Reference soda and soda/AQ pulps were produced.AS/AQ and soda/AQ pulps with kappa numbers below 15 were produced easily. Cooking of corresponding soda pulps under comparable conditions stopped at a kappa number of 25. The characteristics of unbleached AS/AQ, soda/AQ and soda pulps were compared. AS/AQ and soda/AQ pulps had similar tensile strength but tear was in favor to AS/AQ. The latter had much higher yield and brightness.TCF bleaching of AS/AQ pulp was performed in O Q (OP) or O Q (OP) P sequences. Oxygen delignification (O) resulted in a delignification degree of approximately one third. Followed by a chelating treatment (Q) and a subsequent (OP) stage under drastic conditions a brightness of 82.7% ISO was achieved in an O Q (OP) sequence. The sequence O Q (OP) P, with 2% H2O2 in the final P stage, improved brightness from 77.2% ISO after the OP stage to 83.6% ISO. Bleaching led to only small losses in tensile and burst strength but enhanced tear strength. Short beating in a Jokro mill was sufficient to attain good pulp strength. The results of this study indicate that AS/AQ pulping and TCF bleaching is a convincing alternative to produce high-quality pulp from wheat straw for writing and printing paper. 相似文献
56.
Identification of Manna excreting Aphids
Up to now the origin of Manna in northern Iraq has not been known distinctly. As producers of the sweet excretions called Manna on oak trees were identified the following species of Aphididae:Aphis fabae Scop.,A. gossypii Glov.,Chromaphis juglandicola Kalt.,Myzocallis castanicola Bak.,Tuberculatus annulata Htg.,T. moerickei Hrl. andThelaxes sp. 相似文献
57.
Pseudophrynamine, lunacridine and the new compound 2-(2',4',6'-trimethyl-heptenyl)-4-quinozolone were isolated from the leaves of Zanthoxylum budrunga. 相似文献
58.
One myrsinol-type diterpene ester (1) isolated from Euphorbia decipiens was evaluated for analgesic activity in the acetic acid induced writhing test in mice. Different dose (5-20 mg/kg i.p.) of the compound showed significant antinociceptive activity, which was comparable to standard analgesic drugs, aspirin and ibuprofen (100 mg/kg i.p.). 相似文献
59.
Four known coumarins, coumarin (1), 7-hydroxy-3-methylcoumarin (2), oreoselone (3) and heraclenin (4), were isolated from aerial part of Halocnemum strobilaceum. Their structures were determined by 1 and 2-D NMR techniques. 相似文献
60.
Effect of different levels of dietary sweet orange (Citrus sinensis) peel extract on humoral immune system responses in broiler chickens 下载免费PDF全文
Zohreh Pourhossein Ali Ahmad Alaw Qotbi Alireza Seidavi Vito Laudadio Gerardo Centoducati Vincenzo Tufarelli 《Animal Science Journal》2015,86(1):105-110
This experiment was conducted to evaluate the effects of different levels of sweet orange (Citrus sinensis) peel extract (SOPE) on humoral immune system responses in broiler chickens. Three hundred 1‐day broilers (Ross‐308) were randomly allocated to treatments varying in supplemental SOPE added in the drinking water. The experimental groups consisted of three treatments fed for 42 days as follows: a control treatment without feed extract, a treatment containing 1000 ppm of SOPE and a treatment containing 1250 ppm of SOPE. All treatments were isocaloric and isonitrogenous. Broilers were vaccinated with Newcastle disease virus (NDV), avian influenza (AI), infectious bursal disease (IBD) and infectious bronchitis virus (IBV) vaccines. Antibody titer response to sheep red blood cells (SRBC) was higher in the group fed 1250 ppm of SOPE (P < 0.05) as well as for immunoglobulin G (IgG) and IgM. Similarly, antibody titer responses to all vaccines were constantly elevated (P < 0.05) by SOPE enrichment in a dose‐dependent manner. Relative weights of spleen and bursa of Fabricius were unaffected by treatments. Dietary SOPE supplementation may improve the immune response and diseases resistance, indicating that it can constitute a useful additive in broiler feeding. Thus, supplying SOPE in rations may help to improve relative immune response in broiler chickens. 相似文献