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21.
OBJECTIVE: To investigate the development of enrofloxacin resistance among Escherichia coli isolates obtained from chickens by determining mutant-prevention concentrations (MPCs) and sequence the quinolone resistance-determining regions (QRDRs) of gyrA and parC genes in selected isolates. SAMPLE POPULATION: 15 chicken-derived E coli isolates. PROCEDURES: For all isolates, MPC and minimal inhibition concentration (MIC) of enrofloxacin were determined. The MPCs and maximum serum drug concentrations attained with enrofloxacin doses recommended for treatment of E coli infections in chickens were compared. Mutation frequencies and QRDR sequence changes in gyrA and parC were also determined. RESULTS: In 2 of 15 E coli strains, MPCs were low (0.016 and 0.062 microg/mL), MPC:MIC ratios were 2 and 4, and the GyrA and ParC proteins had no mutations. In 9 susceptible isolates with a GyrA point mutation, MPCs ranged from 2 to 16 microg/mL. For isolates with double mutations in GyrA and a single mutation in ParC, MPCs were > 32 microg/mL (several fold greater than the maximal plasma concentration of enrofloxacin in chickens); mutation frequencies were also much lower, compared with frequencies for single-mutation isolates. CONCLUSIONS AND CLINICAL RELEVANCE: For E coli infections of chickens, MPC appears to be useful for determining enrofloxacin-dosing strategies. The high MPC:MIC ratio may result in enrofloxacin-treatment failure in chickens infected with some wild-type gyrA E coli isolates despite the isolates' enrofloxacin susceptibility (MICs 0.125 to 1 microg/mL). For infections involving isolates with high MPCs, especially those containing mutations in gyrA and parC genes, treatment with combinations of antimicrobials should be adopted. 相似文献
22.
1. An experiment was conducted to study the effect of thiram on liver antioxidant capacity and incidence of tibial dyschondroplasia in broilers. 2. One hundred and twenty Avian commercial broilers were allotted at random to three treatments: control group, low thiram group (50 mg/kg) and high thiram group (100 mg/kg). 3. Blood samples were collected to determine the activity of AST (aspartate aminotransferase). At the end of the trial, broilers were killed and liver samples were collected to determine the activity of SOD (superoxide dismutase), GSH-Px (glutathione peroxidase) and MDA (malondialdehyde) content, while the right proximal tibiotarsi were dissected in longitudinal section for assessment of tibial dyschondroplasia (TD) incidence and TD score. 4. The results showed that thiram increased the incidence of TD and TD scores, increased serum AST activity and MDA content of liver, and decreased the activity of SOD and GSH-Px in the liver. 5. They suggest that thiram causes TD in broilers by reducing liver antioxidation capability and damaging liver function; this may be one of the mechanisms by which thiram causes TD in broilers. 相似文献
23.
The design of Bio-clean operating room in modern hospital is quite different from that of traditional operating room. This paper discusses three aspects of Bio-clean operating room design: air clean standard and air purification, interior equipment system and interior finish. It is hoped that this discussion will conduce to later designs. 相似文献
24.
In order to upgrade traditional industry and realize the automation of furnace condition judgement and standardization of operation, an expert system for various furnace conditions and breakdown judgement and operation guidance on blast furnace are developed based on the theory of artificial intelligence and knowledge engineering. The system is fulfilled through C++Builder and combined with the actual production picture. The system has efficient and convenient man-machine interface by using menu operation method. The testing and operation of actual production data in simulation system indicate that the system is exact and reliable and can provide both the warning of various furnace breakdown and corresponding operation guidance. 相似文献
25.
The turbogenerator vibration faults have the character of variety. Many faults often occur synchronously. This paper introduces a diagnosing model based on parsimonious covering theory and probability. A model for turtogenerator's fault diagnosis is proposed. The availability of this method is proved by two fault diagnosis examples of turbogenerator. The results show that the model proposed can be used for multi_fault diagnosis together. It may make up shortage for some of expert systems and neural networks in some aspect. From the practice,this model has higher reliability and practicability. 相似文献
26.
半粒稻米直链淀粉测定方法的研究及应用 总被引:7,自引:0,他引:7
本文提出一个简便易行的分析方法,用1粒稻米既可测出其中的直链淀粉含量,还可用以繁种,便于在育种早代选择优良个体、提高效率,加快进程。用本方法测得的直链淀粉含量与用中华人民共和国国标法GB_(7648-87),测得的结果经t检验,差异不显著。利用本法分析了F_1代种子及其亲本的直链淀粉含量,结果表明,在非糯品种与糯稻品种的两 相似文献
27.
土工织物导流工程应实际工程的需要而产生,在软基河流上运用土工织物导流,需要根据其特点来设计和施工。本文概述了土工织物导流工程的设计要点和施工工艺,并提出了防老化措施。 相似文献
28.
通过系统分析柴油机螺旋气道几何结构以及气道敏感区域对气道流通性能的影响关系,确定了构建螺旋气道的关键结构参数。提出了一种以气缸盖结构为约束条件,涡流比为设计目标,再进行流量系数优化的螺旋气道数字化设计方法,建立了螺旋气道参数化数学模型,开发了一款柴油机螺旋气道数字化建模软件。针对一款自主开发的卧式两缸柴油机螺旋气道的设计要求,采用螺旋气道数字化建模软件建立了螺旋气道数字化三维模型,进行了模型光顺性分析和流通性能分析。分析结果表明,螺旋气道数字化设计方法具有一定的可行性和通用性,只需提供气缸盖结构、流通性能(涡流比和流量系数)的设计要求,通过该数字化设计方法和建模软件就能设计出满足要求的螺旋气道。 相似文献
29.
3100QB型柴油机振动测试与分析 总被引:1,自引:1,他引:1
针对3100QB柴油机的离合器壳在高速行驶工况易出现径向开裂现象,通过各试验工况下的多测点测试及幅值谱的振动分析,找出了3100QB柴油机自身的振动力源及导致离合器壳开裂的主要原因,并提出现有条件下减振的有效技术与措施。 相似文献
30.