全文获取类型
收费全文 | 76359篇 |
免费 | 3844篇 |
国内免费 | 51篇 |
专业分类
林业 | 3595篇 |
农学 | 2341篇 |
基础科学 | 392篇 |
7949篇 | |
综合类 | 15522篇 |
农作物 | 3080篇 |
水产渔业 | 3395篇 |
畜牧兽医 | 38507篇 |
园艺 | 904篇 |
植物保护 | 4569篇 |
出版年
2018年 | 856篇 |
2017年 | 925篇 |
2016年 | 856篇 |
2015年 | 779篇 |
2014年 | 952篇 |
2013年 | 2600篇 |
2012年 | 1678篇 |
2011年 | 2073篇 |
2010年 | 1359篇 |
2009年 | 1285篇 |
2008年 | 2038篇 |
2007年 | 2079篇 |
2006年 | 1897篇 |
2005年 | 1835篇 |
2004年 | 1759篇 |
2003年 | 1782篇 |
2002年 | 1697篇 |
2001年 | 2215篇 |
2000年 | 2219篇 |
1999年 | 1846篇 |
1998年 | 700篇 |
1997年 | 698篇 |
1995年 | 812篇 |
1993年 | 696篇 |
1992年 | 1489篇 |
1991年 | 1551篇 |
1990年 | 1666篇 |
1989年 | 1644篇 |
1988年 | 1527篇 |
1987年 | 1477篇 |
1986年 | 1527篇 |
1985年 | 1515篇 |
1984年 | 1247篇 |
1983年 | 1144篇 |
1982年 | 771篇 |
1981年 | 717篇 |
1979年 | 1248篇 |
1978年 | 979篇 |
1977年 | 848篇 |
1976年 | 803篇 |
1975年 | 913篇 |
1974年 | 1201篇 |
1973年 | 1240篇 |
1972年 | 1255篇 |
1971年 | 1182篇 |
1970年 | 1102篇 |
1969年 | 1003篇 |
1968年 | 863篇 |
1967年 | 906篇 |
1966年 | 837篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
221.
222.
Acute and chronic hormone and metabolite changes in lambs fed the beta-agonist, cimaterol 总被引:1,自引:0,他引:1
R.M. O''Connor W.R. Butler K.D. Finnerty D.E. Hogue D.H. Beermann 《Domestic animal endocrinology》1991,8(4):537-548
The objective of this study was to determine if acute and chronic changes in circulating metabolic hormone and metabolite concentrations are associated with β-agonist-induced nutrient repartitioning in young growing lambs. Two groups of 12 Dorset and Dorset-Finn cross ram lambs weighing 36 or 33 kg live weight were assigned to 3- or 6-week treatment intervals, respectively, to achieve similar slaughter weights. Six lambs within each treatment interval were fed ad libitum a complete mixed high-concentrate diet containing either 0 or 10 ppm cimaterol. During the first 12 hr of cimaterol administration plasma somatotropin (ST), thyroxine (T4), and triiodothyronine (T3) concentrations were not altered by treatment, but plasma insulin, glucose, non-esterified fatty acids (NEFA) and glycerol concentrations were elevated 2 hr after ingestion. These acute responses suggest direct stimulation of glycogenolysis and lipolysis by cimaterol, which is characteristic of β-adrenergic alteration of carbohydrate and lipid metabolism. Chronic administration of cimaterol significantly decreased insulin concentrations by 36% and 52% at 3 and 6 weeks, respectively, while glucose concentrations remained unchanged. Serum IGF-I concentrations were not significantly altered by cimaterol. T4 levels were reduced 22.1% after 3 weeks of cimaterol treatment. Although plasma NEFA concentrations were chronically elevated 56% to 65% in lambs fed cimaterol, plasma glycerol concentrations remained at baseline levels. The relative changes in plasma NEFA and glycerol concentrations are consistent with a decreased rate of lipogenesis, rather than an increase in lipolysis. 相似文献
223.
224.
The occurrence of hide damage light flecks and spots was determined on tanned hides from 28 herds during a period of 8 to 12 months. Light flecks and spots are described as small areas of grain loss up to 3 mm in diameter that are seen on dyed crust cattle leather. Damage was found on 75.8% of all hides. The neck and shoulders were the anatomical region with the highest prevalence of damage. Sixty-eight per cent of all hides had light flecks and spots in this region. The forelimbs and dewlap were the anatomical region with the second highest occurrence with a prevalence of 39.1%. This distribution corresponded to the known distribution of lice in cattle. No significant differences were observed in age, sex, prevalence of lice in the herd assessed in March or infestations with different lice species. The frequency of light flecks and spots varied significantly during the year. The frequency was highest in the late winter and early spring, decreased significantly during the summer and was lowest in the autumn. This variation supported the importance of lice in the development of light flecks and spots and suggested a relatively long healing period for the damages induced by lice. 相似文献
225.
Immunohistochemical detection of scrapie prion proteins in clinically normal sheep in Pennsylvania. 总被引:2,自引:0,他引:2
H Kim K I O'Rourke M Walter H G Purchase J Enck T K Shin 《Journal of veterinary diagnostic investigation》2001,13(1):89-91
Following diagnosis of scrapie in a clinically suspect Suffolk sheep, 7 clinically normal flockmates were purchased by the Pennsylvania Department of Agriculture to determine their scrapie status using an immunohistochemical procedure. Two of the 7 euthanized healthy sheep had positive immunohistochemical staining of the prion protein of scrapie (PrP-Sc) in their brains, nictitating membranes, and tonsils. The PrP-Sc was localized in the areas of the brain where, histopathologically, there was neurodegeneration and astrocytosis. The PrP-Sc occurred within germinal centers of the affected nictitating membranes and tonsils and was located in the cytoplasm of the dendrite-like cells, lymphoid cells, and macrophages. These results confirm that immunohistochemical examination of the nictitating membrane can be used as a screen for the presence of scrapie infection in clinically normal sheep at a capable veterinary diagnostic laboratory. In sheep with a PrP-Sc-positive nictitating membrane, the diagnosis of scrapie should be confirmed by histopathology and immunohistochemical examination of the brain following necropsy. Following full validation, immunohistochemistry assays for detection of PrP-Sc in nictitating membrane lymphoid tissues can improve the effectiveness of the scrapie control and eradication program by allowing diagnosis of the disease in sheep before the appearance of clinical signs. 相似文献
226.
AM Mwanza A Madej H Kindahl N Lundeheim & S Einarsson 《Reproduction in domestic animals》2001,36(3-4):157-161
The effects of lipopolysaccharide ( Escherichia coli , O55:B5), administered 18 h after ovulation in the second oestrus after weaning on the hormonal profiles in 14 Swedish cross-bred (Landrace × Yorkshire) multiparous sows were studied. The endotoxin group (E-group) sows were administered with 300 ng/kg of lipopolysaccharide (LPS) whereas the control group (C-group) sows were administered 5 ml of saline intravenously via an indwelling jugular cannula. Blood samples for hormonal analyses were collected from all sows until slaughter. In the E-group, progesterone, cortisol and prostaglandin F2α metabolite levels increased significantly (p < 0.05) following LPS compared with the C-group. It can be concluded from this study that apart from elevating cortisol and prostaglandin F2α metabolite, LPS also elevates progesterone levels. 相似文献
227.
228.
H K Pradhan G C Mohanty W Y Lee B Patnaik 《Veterinary immunology and immunopathology》1991,29(3-4):229-238
Antibody directed against Marek's disease-associated tumor surface antigen (MATSA) was eluted from tumor cells of lymphomas and peripheral blood lymphocytes that were isolated from Marek's disease virus-infected chickens. Feather follicular Marek's disease virus (MDV) antigen could not be demonstrated with this antibody by indirect immunofluorescent (IF) staining. Monoclonal antibody directed against MATSA could completely block the activity of eluted antibody and vice versa. By indirect IF staining using eluted antibody and fluorescein isothiocyanate (FITC) labelled antichicken globulin conjugate. MATSA-bearing cells were detected in MDV infected and herpes virus of turkey (HVT) vaccinated birds. Blocking of immunoglobulin molecules present on B-cells by anti-chicken globulin is critical in this test. 相似文献
229.
230.
Fluid accumulation in mouse ligated intestine inoculated with the vascular permeability factor produced by Bacillus cereus. 总被引:1,自引:0,他引:1
K Shinagawa K Sato H Konuma N Matsusaka S Sugii 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(2):167-171
Partially purified vascular permeability (VP) factor (VPF) of Bacillus cereus induced fluid accumulation in the ligated intestinal loops of mouse (MIL) and rabbit (RIL), suggesting that the VP activity may correlate with fluid accumulation in ligated intestinal loops of these animals. Fluid accumulation was observed at 6-8 hr in 55-67% of mouse intestinal loops inoculated with 40-50 immunodiffusion units (IDU) of partially purified VPF, whereas it was found at 2 hr in all loops with 400-600 IDU of partially purified VPF. In rabbit intestinal loops with 120-190 IDU of partially purified VPF, fluid accumulation was observed at 6 hr. From these findings, VPF produced by B. cereus can be easily detected in both MIL and RIL. The intestinal tissue of mouse intestinal loops was histopathologically damaged at different concentrations of the VPF to induce fluid accumulation. With 50 IDU of partially purified VPF, severe edema was found in the laminia proprial layer and submucosa. With 600 IDU of partially purified VPF, on the other hand, severe necrosis in the surface epithelium of villus and laminia proprial layer, and hyperemia in the submucosa were observed, suggesting that partially purified VPF may be cytotoxic and/or intestinecrotic. 相似文献