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171.
172.
Jessica L. Butler Peter J. Bottomley Stephen M. Griffith 《Soil biology & biochemistry》2004,36(2):371-382
The cycling of root-deposited photosynthate (rhizodeposition) through the soil microbial biomass can have profound influences on plant nutrient availability. Currently, our understanding of microbial dynamics associated with rhizosphere carbon (C) flow is limited. We used a 13C pulse-chase labeling procedure to examine the flow of photosynthetically fixed 13C into the microbial biomass of the bulk and rhizosphere soils of greenhouse-grown annual ryegrass (Lolium multiflorum Lam.). To assess the temporal dynamics of rhizosphere C flow through the microbial biomass, plants were labeled either during the transition between active root growth and rapid shoot growth (Labeling Period 1), or nine days later during the rapid shoot growth stage (Labeling Period 2). Although the distribution of 13C in the plant/soil system was similar between the two labeling periods, microbial cycling of rhizodeposition differed between labeling periods. Within 24 h of labeling, more than 10% of the 13C retained in the plant/soil system resided in the soil, most of which had already been incorporated into the microbial biomass. From day 1 to day 8, the proportion of 13C in soil as microbial biomass declined from about 90 to 35% in rhizosphere soil and from about 80 to 30% in bulk soil. Turnover of 13C through the microbial biomass was faster in rhizosphere soil than in bulk soil, and faster in Labeling Period 1 than Labeling Period 2. Our results demonstrate the effectiveness of using 13C labeling to examine microbial dynamics and fate of C associated with cycling of rhizodeposition from plants at different phenological stages of growth. 相似文献
173.
DNA structure: evidence from electron microscopy 总被引:9,自引:0,他引:9
J D Griffith 《Science (New York, N.Y.)》1978,201(4355):525-527
The contour lengths of phiX174 DNA duplex and RNA-DNA hybrid molecules were measured by several commonly used electron microscopic techniques. The countour length of the hybrid molecules corresponds to a rise of 2.5 to 2.6 angstroms per base pair, as expected for the A conformation, while the length of phiX174 duplex DNA similarly measured corresponds to a 2.9-angstrom rise, very different from 3.4 angstroms of the classic B form. Thus any chromatin structure parameter based on electron microscopy and a rise of 3.4 angstroms must be reappraised. The possibility that DNA in dilute solution also has a rise of 2.9 angstroms and a screw of 10.5 base pairs per turn is discussed. 相似文献