Best practice for the pharmacological management of hyperthyroid cats with antithyroid drugs

Pharmacological management of feline hyperthyroidism offers a practical treatment option for many hyperthyroid cats. Two drugs have been licensed for cats in the last decade: methimazole and its pro‐drug carbimazole. On the basis of current evidence and available tablet sizes, starting doses of 2·5 mg methimazole twice a day and 10 to 15 mg once a day for the sustained release formulation of carbimazole are recommended. These doses should then be titrated to effect in order to obtain circulating total thyroxine (TT4) concentrations in the lower half of the reference interval. Treated cases should be monitored for side‐effects, especially during the first months of treatment. Some side‐effects may require discontinuation of treatment. At each monitoring visit, clinical condition and quality of life should also be evaluated, with special attention to possible development of azotaemia, hypertension and iatrogenic hypothyroidism. When euthyroidism has been achieved, monitoring visits are recommended after 1 month, 3 months and biannually thereafter. Cats with pre‐existing azotaemia have shorter survival times. However, development of mild azotaemia during the initial course of treatment, unless associated with hypothyroidism, does not appear to decrease survival time. The long‐term effects of chronic medical management require further study .     

Residual marine protected areas five years on: Are we still favouring ease of establishment over need for protection?

• 1. Marine protected areas (MPAs) are today's cornerstone of many marine conservation strategies. Our 2015 study (Devillers et al., 2015) and others have shown, however, that the placement of MPAs is ‘residual’ to commercial uses and biased towards areas of lower economic value or interest.
• 2. In this paper, we explored the impact of our study on marine science, policy and management practice.
• 3. We reviewed the papers citing our work and compiled expert opinions on some of the impacts of our study.
• 4. Results indicate a strong general uptake in the scientific community but more uneven impacts on policy and management in different contexts, with a likely smaller impact of the research on conservation practice.

     

A genome scan of a four-way tilapia cross supports the existence of a quantitative trait locus for cold tolerance on linkage group 23

A genome scan, searching for quantitative trait loci (QTL) for the traits cold tolerance and body weight in tilapia, was performed on a cross between a (Oreochromis niloticus×Sarotherodon galilaeus) male and a (O. mossambicus×O. aureus) female. Fifty‐four microsatellites and 23 amplified fragment length polymorphism (AFLP) primer combinations were genotyped and tested for marker–trait associations. Sex‐specific linkage maps were constructed from this data. Twenty‐three point‐wise significant marker–trait associations were found in the genome scan, and putative QTL were subsequently tested in another (On×Sg) × (Om×Oa) family. None of the putative QTL from the first experiment were significant in the second experiment. However, one microsatellite, UNH130, found to be associated to weight in the first experiment, was found to be strongly associated to cold tolerance in the second experiment. Since QTL for cold tolerance and body weight were recently found on the linkage group containing UNH130 (linkage group 23) in another study, this linkage group was investigated more closely using interval mapping. The results provide indications, but not conclusive evidence, of a QTL for cold tolerance on linkage group 23.     

Longitudinal serological surveys of Atlantic salmon, Salmo salar L., using a rapid immunoperoxidase-based neutralization assay for salmonid alphavirus

Longitudinal serological surveys for salmon pancreas disease virus (SPDV), the causal agent of pancreas disease (PD), were conducted on multiple caged populations of Atlantic salmon, Salmo salar L., on two farms over a 77-week period (farm 1, freshwater and marine stages) and a 36-week period (farm 2, marine stage only), using a microtitre-based virus neutralization (VN) assay. Collected sera were also screened for viraemia with SPDV, and pancreas, heart and muscle tissues were examined for lesions consistent with PD. Outbreaks of PD occurred during the marine phase on both farms, as demonstrated by seroconversion, the isolation of virus and progressive histopathological changes consistent with a PD outbreak. All populations monitored showed a progressive increase in seroprevalence of 90-100%, typically accompanied by rises in geometric mean antibody titres. With the exception of one caged population, which showed a marked biphasic seroprevalence pattern, the seroprevalence figures in the remaining four monitored populations remained high (> or =70%) until the end of the study period. Peak VN titres of > or =1/1280 were detected on both farms. The results provide essential baseline information for the interpretation of SPDV VN serology results, and indicate that this methodology is suited to both the diagnosis and seroepidemiology of SPDV infections.     

Effects of combined applications of ammonium thiosulphate and fungicides on fruit load and blossom blight and their phytotoxicity to peach trees

Summary

Timing of bloom sprays of ammonium thiosulphate (ATS) for foliar fertilization and fruit thinning, and of fungicides for disease control, is critical in peach production. Initial observations in commercial orchards indicated that ATS and fungicides, when applied as tank mixes, could cause burn of blossoms and shoots, but not when applied separately. Nine fungicides, alone and in combination with ATS, were evaluated on single tree plots in 1993 and five representative treatments were repeated in 1994 under commercial orchard conditions. Treatments were applied by hand-gun from a 100 litre sprayer in 1993 and by air-blast sprayer in 1994. In 1993, fruit number per tree was affected only by ATS and the numbers of blossom blight cankers per tree only by fungicide, with no interaction between ATS and fungicides. There were however some ATS × fungicide interaction regarding phytotoxicity to blossom and one-year old shoots, but the damage to the latter was slight. In 1994 blossom blight did not occur and there was no evidence of phytotoxicity to shoots or blossoms. Fruit number was not affected by any treatment.     

Modelling the effects of timing and rates of application of benzyladenine as a secondary thinner of ‘Fuji’ apple after ethephon

A trial on ‘Fuji’ apples at the Grove Research Station in southern Tasmania during the 1991/92 season studied the thinning effect and interactions between ethephon and benzyladenine (BA) when BA was applied as a secondary thinner after a full bloom (FB) application of ethephon. The spray treatments were a factorial combination of eight application times of BA (11,13,15,17,19, 21, 23 or 25 days after full bloom (AFB)) with ten concentrations (20,40,60,80,100,120,140,160,180 or 200 mg I“1). An unsprayed control and an ethephon control were included. Target thinning results were achieved 19- 23 d AFB with concentrations of 140-160 mg I“1. A corollary of this successful thinning was an increase in fruit weight and size. Return bloom was improved significantly where thinning was successful. There was no effect on fruit firmness, soluble solids or lateral branching. Drawbacks were an increase in the incidence of russet and a reduction in pip number at the optimum thinning times and concentrations.     

Factors limiting the synthesis of virgin olive oil volatile esters

The aim of the present work was to establish the limiting factors affecting the biosynthesis of volatile esters present in virgin olive oil (VOO). Oil volatile fractions of the main Spanish olive cultivars, Arbequina and Picual, were analyzed. It was observed that acetate esters were the most abundant class of volatile esters in the oils, in concordance with the high content of acetyl-CoA found in olive fruit, and that the content of C6 alcohols is limited for the synthesis of volatile esters during the production of VOO. Thus, the increase of C6 alcohol availability during VOO production produced a significant increase of the corresponding ester in the oils in both cultivars at two different maturity stages. However, the increase of acetyl-CoA availability had no effect on the VOO volatile fraction. The low synthesis of these C6 alcohols seems not to be due to a shortage of precursors or cofactors for alcohol dehydrogenase (ADH) activity because their increase during VOO production had no effect on the C6 alcohol levels. The experimental findings are compatible with a deactivation of ADH activity during olive oil production in the cultivars under study. In this sense, a strong inhibition of olive ADH activity by compounds present in the different tissues of olive fruit has been observed.     

Evaluation of serial venous and arterial lactate concentrations in healthy anesthetized sheep undergoing ovariectomy

ObjectiveTo determine if lactate concentrations in jugular venous and auricular arterial blood differ in anesthetized sheep.Study designProspective, controlled experimental study.AnimalsTwelve healthy adult ewes, 4–7 years and weighing 62–77 kg.MethodsJugular venous blood was collected before anesthesia (PreO_v) for measurement of lactate concentration, packed cell volume and total protein. Ewes were administered a standard anesthesia protocol. Jugular venous (IntraO_v) and auricular arterial (IntraO_a) blood samples were obtained 40 minutes after induction of anesthesia, and again in recovery (PostO_v and PostO_a). An additional blood sample was drawn 6 weeks post-operatively from non-fasted sheep (NF_Lact). Lactate concentrations were compared among PreO_v, IntraO_v and IntraO_a, PostO_v and PostO_a, and between PreO_v and NF_Lact with paired t-test and repeated measure analyses of variance (anova) with PreO_v as a covariate (p = 0.05).ResultsIntraO_v lactate concentration had decreased from PreO_v There were significant differences between arterial and venous IntraO and PostO lactate concentrations. There was no significant difference between IntraO and PostO, or PreO_v and NF_Lact.Conclusions and clinical relevanceLactate concentrations were significantly lower in anesthetized sheep compared to non-anesthetized sheep. Lactate concentrations in venous blood were higher than in arterial blood. Therefore, anesthetic status and sampling site should be considered when interpreting lactate concentrations, and the sampling site should be consistent for repeated measurements.