The cause of decreased pan evaporation over the past 50 years

Changes in the global water cycle can cause major environmental and socioeconomic impacts. As the average global temperature increases, it is generally expected that the air will become drier and that evaporation from terrestrial water bodies will increase. Paradoxically, terrestrial observations over the past 50 years show the reverse. Here, we show that the decrease in evaporation is consistent with what one would expect from the observed large and widespread decreases in sunlight resulting from increasing cloud coverage and aerosol concentration.     

Mesenteric strangulation by pedunculated lipomas without involvement of associated intestine in four horses

An unusual form of strangulation of mesentery only by pedunculated lipoma was identified as the cause of moderate to severe colic in four horses. The small intestinal mesentery was involved in three horses and the small colon mesentery in the fourth horse, but the associated intestine was not strangulated, haemorrhagic, nor obviously obstructed in any horse. In all horses, a patch of haemorrhagic mesentery of variable size was evident around the origin of a mesenteric lipoma and a major mesenteric vessel. One horse was subjected to euthanasia at the owner's request and the remaining three had surgery from which they made a complete recovery after the removal of the lipoma. Intestinal resection was not performed in any of the three horses undergoing surgery. An isolated mesenteric strangulation by lipoma is an uncommon cause of colic in older horses that have the typical presentation of a strangulating lipoma. The removal of the involved lipoma without mesenteric or intestinal resection can be associated with complete recovery.     

Soil-geomorphic significance of land surface characteristics in an arid mountain range,Mojave Desert,USA

Mountains comprise an extensive and visually prominent portion of the landscape in the Mojave Desert, California. Landform surface properties influence the role these mountains have in geomorphic processes such as dust flux and surface hydrology across the region. The primary goal of this study was to describe and quantify land surface properties of arid-mountain landforms as a step toward unraveling the role these properties have in soil-geomorphic processes. As part of a larger soil-geomorphic study, four major landform types were identified within the southern Fry Mountains in the southwestern Mojave Desert on the basis of topography and landscape position: mountaintop, mountainflank, mountainflat (intra-range low-relief surface), and mountainbase. A suite of rock, vegetation, and morphometric land surface characteristic variables was measured at each of 65 locations across the study area, which included an associated piedmont and playa. Our findings show that despite the variation within types, landforms have distinct land surface properties that likely control soil-geomorphic processes. We hypothesize that surface expression influences a feedback process at this site where water transports sediment to low lying areas on the landscape and wind carries dust and soluble salts to the mountains where they are washed between rocks, incorporated into the soil, and retained as relatively long-term storage. Recent land-based video and satellite photographs of the dust cloud emanating from the Sierra Cucapá Mountains in response to the 7.2-magnitude earthquake near Mexicali, Mexico, support the hypothesis that these landforms are massive repositories of dust.     

Analysis of the acute phase responses of Serum Amyloid A, Haptoglobin and Type 1 Interferon in cattle experimentally infected with foot-and-mouth disease virus serotype O

ABSTRACT: A series of challenge experiments were performed in order to investigate the acute phase responses to foot-and-mouth disease virus (FMDV) infection in cattle and possible implications for the development of persistently infected "carriers". The host response to infection was investigated through measurements of the concentrations of the acute phase proteins (APPs) serum amyloid A (SAA) and haptoglobin (HP), as well as the bioactivity of type 1 interferon (IFN) in serum of infected animals. Results were based on measurements from a total of 36 infected animals of which 24 were kept for observational periods exceeding 28 days in order to determine the carrier-status of individual animals. The systemic host response to FMDV in infected animals was evaluated in comparison to similar measurements in sera from 6 mock-inoculated control animals.There was a significant increase in serum concentrations of both APPs and type 1 IFN in infected animals coinciding with the onset of viremia and clinical disease. The measured parameters declined to baseline levels within 21 days after inoculation, indicating that there was no systemically measurable inflammatory reaction related to the carrier state of FMD. There was a statistically significant difference in the HP response between carriers and non-carriers with a lower response in the animals that subsequently developed into FMDV carriers. It was concluded that the induction of SAA, HP and type 1 IFN in serum can be used as markers of acute infection by FMDV in cattle.     

Maintenance, spillover and spillback transmission of bovine tuberculosis in multi-host wildlife complexes: a New Zealand case study

The causative agent of bovine tuberculosis (bTB; Mycobacterium bovis) has a broad host range. The role of each animal species in spreading the disease depends on how transmission occurs, on the abundance of each host, and on the interactions between hosts. This paper explores differences in the roles individual host species can play in allowing M. bovis infection to persist and spread within a multi-species complex, using New Zealand as a case study. In New Zealand, four wild mammal species are frequently infected. Of these the brushtail possum is now regarded as the only true "maintenance" host. Red deer and ferrets can become maintenance hosts where their densities are exceptionally high, but more often they are "spillover" hosts, with most infection arising from moderately frequent inter-species transmission from possums. The latter situation is even more strongly the case for feral pigs. Spillover hosts may occasionally play a crucial epidemiological role by transmitting infection back to a potential maintenance host (spillback). Three key factors make spillback transmission far more epidemiologically important than its low frequency of occurrence might suggest--amplification of the reservoir of bTB, far greater spatial spread than by the maintenance host, and greater persistence of bTB in long-lived spillover hosts extending the risk of spillback far into the future. The risk of spillback is undoubtedly low, but it nonetheless determines the nature, scale and duration of management required. Eradication of the disease may require management of both the infection in maintenance hosts and reduction or elimination of any risk of spillback.     

High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex crop genomes

Background

Targeted Induced Loci Lesions IN Genomes (TILLING) is increasingly being used to generate and identify mutations in target genes of crop genomes. TILLING populations of several thousand lines have been generated in a number of crop species including Brassica rapa. Genetic analysis of mutants identified by TILLING requires an efficient, high-throughput and cost effective genotyping method to track the mutations through numerous generations. High resolution melt (HRM) analysis has been used in a number of systems to identify single nucleotide polymorphisms (SNPs) and insertion/deletions (IN/DELs) enabling the genotyping of different types of samples. HRM is ideally suited to high-throughput genotyping of multiple TILLING mutants in complex crop genomes. To date it has been used to identify mutants and genotype single mutations. The aim of this study was to determine if HRM can facilitate downstream analysis of multiple mutant lines identified by TILLING in order to characterise allelic series of EMS induced mutations in target genes across a number of generations in complex crop genomes.

Results

We demonstrate that HRM can be used to genotype allelic series of mutations in two genes, BraA.CAX1a and BraA.MET1.a in Brassica rapa. We analysed 12 mutations in BraA.CAX1.a and five in BraA.MET1.a over two generations including a back-cross to the wild-type. Using a commercially available HRM kit and the Lightscanner? system we were able to detect mutations in heterozygous and homozygous states for both genes.

Conclusions

Using HRM genotyping on TILLING derived mutants, it is possible to generate an allelic series of mutations within multiple target genes rapidly. Lines suitable for phenotypic analysis can be isolated approximately 8-9 months (3 generations) from receiving M₃ seed of Brassica rapa from the RevGenUK TILLING service.     

Universal endogenous gene controls for bisulphite conversion in analysis of plant DNA methylation

Background

Protein extraction is a frequent procedure in biological research. For preparation of plant cell extracts, plant materials usually have to be ground and homogenized to physically break the robust cell wall, but this step is laborious and time-consuming when a large number of samples are handled at once.

Results

We developed a chemical method for lysing Arabidopsis cells without grinding. In this method, plants are boiled for just 10 minutes in a solution containing a Ca²⁺ chelator and detergent. Cell extracts prepared by this method were suitable for SDS-PAGE and immunoblot analysis. This method was also applicable to genomic DNA extraction for PCR analysis. Our method was applied to many other plant species, and worked well for some of them.

Conclusions

Our method is rapid and economical, and allows many samples to be prepared simultaneously for protein analysis. Our method is useful not only for Arabidopsis research but also research on certain other species.