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71.
Transfer of the stenohaline catfish, Heteropneustes fossilis from tap water (TW) to deionized water (DW) resulted in an increase in the glomerular filtration rate, urine volume and osmolar
and free water clearance. In a closed system, where the DW was renewed only once a day, no change in the plasma osmolality
was evident for up to 14 days. When DW was renewed four times a day for 25 days, a significant reduction in the plasma osmolality
was observed within 24h. When the fish were transferred back to TW, plasma osmolality increased to normal freshwater level
within 24h. These observations suggest the existence of highly efficient branchial mechanisms for active uptake of salts from
an exceedingly dilute ambient medium. The fact that prolactin-secreting cells as well as corticotrophs in the pituitary of
the fish in DW were highly stimulated suggests the involvement of the hormones in the adaptive responses of the catfish to
DW. 相似文献
72.
Estrogen administration in C. punctatus increases RNA: protein and RNA: DNA ratios and depletes glycogen in the liver. Increase in glucose-6-phosphatase activity accounts for glycogen depletion whereas high activity of pyruvate kinase suggests stimulation of the glycolytic pathway at the pyruvate step to generate ATP and to provide carbon skeleton for the vitellogenin molecule. Five-fold increase in the activity of NADP+-dependant malate dehydrogenase generates NADPH possibly for fatty acid synthesis. Additional energy requirement (NADPH) is met by stimulation of hexose monophosphate pathway as well as NADP+-dependant isocitrate dehydrogenase. Increase in the activity of NADPH-cytochrome c reductase in microsomal fraction suggests stimulation of electron transport chain. Increase in the activity of glutamate pyruvate transaminase can be directly related to the synthesis to specific amino acids needed for the formation of vitellogenin whereas reduction in glutamate oxaloacetate suggests extra hepatic source of amino acids. Reduction in the activity of glutamate dehydrogenase may be due to catabolism. 相似文献
73.
Rajendra Phartyal L. Bibekananda Singh S. V. Goswami Neeta Sehgal 《Fish physiology and biochemistry》2005,31(2-3):241-245
Vitellogenin is a female-specific calcium-binding glycolipophosphoprotein synthesized in the hepatocytes of fishes. Its synthesis
can be induced in fishes of either sex by estradiol or by xenoestrogens. To study the in vitro synthesis of vitellogenin,
different culture conditions were set up using the hepatocytes of Clarias gariepinus. The present study reports on a non-enzymatic procedure for isolation and culture of hepatocytes from the liver of the catfish
Clarias gariepinus, in order to study the effects of estradiol on vitellogenin synthesis in vitro. The procedure employs chelating properties
of ethylenediamine tetracetic acid to achieve cell viability in excess of 95%. Equal numbers of isolated cells were incubated
in different culture media viz. RPMI F1640, Medium-199, and Williams’ Medium E. At 36 h, cell attachment and monolayer formation
is faster in M-199 and Williams’ Medium E than in RPMI. In order to study the effects of estradiol on vitellogenin synthesis,
the isolated hepatocytes were seeded in Williams’ Medium E in 24-well cell culture plates. 17 β-estradiol (E2) was introduced in the culture plates at different concentrations and for different time periods. The media were assayed
for vitellogenin using competitive ELISA. Vitellogenin appeared in the medium after 48 h of incubation with 10−5 M estradiol whereas after 72 h of incubation 5×10−7 M E2 could elicit the synthesis. 相似文献
74.
Prabhat Pramanik Manabjyoti Phukan Anup Jyoti Goswami 《Communications in Soil Science and Plant Analysis》2018,49(4):489-498
Tea is an evergreen shrub, and tea bushes (plants) are periodically pruned at 3-year intervals. This practice generates tons of pruning litters (leaves and stems) in tea gardens. In spite of availability, the hard nature and slow decomposition rate limit the use of pruning litters as soil amendments. In this study, an attempt was made to cycle pruning litters in shredded form to evaluate their effect on young tea plants under greenhouse condition. It was observed that shredded prunings were decomposed in soil within study duration and enhanced nitrate-N content in the soil. The study revealed that different parameters like plant height, biomass weight, and N uptake were enhanced due to the application of suitable combinations of pruning litter and urea in the soil. Based on this study, it could be concluded that application of shredded prunings had potential to replace up to 15% of recommended urea during tea cultivation. 相似文献