p53 protein expression in conjunctival squamous cell carcinomas of domestic animals

The expression of p53 protein was investigated in eight formalin-fixed, paraffin-embedded conjunctival squamous cell carcinomas of five horses and one cow, dog and cat each by an immunohistochemical procedure in order to evaluate protein overexpression. Anti-human p53 protein mouse monoclonal antibodies known to be cross-reactive with p53 protein of the animal species examined were used. Positive p53 nuclear immunostaining was detected in five equine, one bovine and one feline cases. Conversely, no p53 immunostaining was found in the only canine case examined. These results demonstrate a frequent p53 overexpression in conjunctival squamous cell carcinoma that could be related to UV-induced mutations of the p53 tumor suppressor gene.     

GIS-models work well, but are not enough: Habitat preferences of Lanius collurio at multiple levels and conservation implications

Species conservation largely depends on knowledge of habitat needs of target species. GIS-models are increasingly used to assess habitat preferences and distribution of target species, but their accuracy is constrained by availability of digital data layers. We developed a two-steps approach aiming at showing pros and cons of landscape (GIS)- and site-level habitat models, identifying key habitat factors for conservation of a threatened bird species, the red-backed shrike Lanius collurio. A spatially explicit GIS-model was generated using landscape variables, and a second model at site level was developed using fine-scale variables measured on the ground. The GIS-based model was then extrapolated to the entire region to obtain a map of distribution of suitable habitats. Positive associations between shrike occurrence and both hedgerow length and partial shrub cover were detected at both scales. Shrikes were also positively associated with grassland cover at landscape level and with partial cover of untilled herbaceous vegetation at the finer scale, and negatively affected by lucerne cover. The GIS-model led to an affordable map of predicted habitat suitability which should help conservationists to focus on different local priorities, but was unable to identify effects of untilled and lucerne cover. Site-level model gave fine details for habitat management, but its application elsewhere requires ground-measurements of factors. Combining the multiscale models could indicate more urgent actions at large scales (e.g. maintaining suitable habitats, or improving connectivity among isolated patches) and draw a detailed figure of the most suitable habitat for the species. Shrike occurrence was associated with a higher number of shrub and tree species: the indicator value of the species should ensure general benefits for biodiversity from dedicated management.     

微波提取法对樱桃、猕猴桃和枸杞多糖特性的影响

微波提取是近年来发展迅速的天然产物提取技术之一。为考察微波提取对多糖得率、结构及生物学功能的影响,论文采用微波功率1 000 W和提取温度140℃,以不同提取时间5,10 和20 min分别对樱桃、猕猴桃和枸杞中多糖进行提取。结果表明,微波提取多糖的得率、相对分子质量和抗氧化性低于传统提取方法,随着微波提取时间延长,多糖相对分子质量逐步降低,但多糖得率与抗氧化性无显著变化;微波提取得到的多糖在单糖组成上与传统提取方法有一定的差异。该研究结果对应用微波提取水果多糖条件的选择及多糖品质控制有一定的参考价值。     

Effect of the nozzle adaptor of sprayer calibrator on flow rate measurements

To ensure the proper use of plant protection products, the European Directive 2009/128/CE requires sprayer inspection throughout Europe. The required tests and methods for inspection of agricultural sprayers are outlined in the European Standard EN 13790: 2003 in which nozzle flow rate measurement is one of the main requirements. Currently, most of the sprayer inspection workshops are equipped with a nozzle flow rate measurement device which consists of air-tight nozzle adaptors connected to 2-3 m hoses which discharge to a bank of graduated cylinders. However, these adaptors block the air holes of the air-induction nozzles, disrupt the spray atomization and change nozzle output. The main objective of this study was to investigate the variation of nozzle flow rate due to the use of air-tight adaptors. A series of nozzle flow rate measurements were made using different kinds of nozzle adaptors with various sizes of air induction, extended range and Turbo TeeJet^® nozzles.The use of air-tight nozzle adaptors increased the flow rates of air induction and extended range nozzles. Plugging the air holes of air-induction nozzles increased the flow rate. The increase in flow rate decreased with nozzle pressure. Overall, the magnitude of the flow rate increase depended on the nozzle type but remained constant for all of the same type. These differences should be accounted for in sprayer calibration.     

The effects of cover cropping on yield and weed control of potato in a transitional system

Cover cropping can have various beneficial effects to the cropping system such us the increase of soil nutrient content and weed suppression. In this respect, the species used for covering is of great importance. This paper reports results on the yield and weed control effects in potato crops preceded by different cover crops over a 2-year period (2003 and 2004) in Central Italy (Viterbo). Results were obtained in the frame of a more complex study set up in 2002 where in a 3-year chick-pea/potato/tomato rotation, each crop was preceded by 7 different soil managements: 5 cover crops (rapeseed, Italian ryegrass, hairy vetch, snail medick and subclover) + 1 unfertilised weedy fallow (cover crop absent) + 1 control (weedy fallow fertilised with mineral N at a rate of 170 kg ha⁻¹ for potato). Two different weed control regimes in potato were also applied [weed-free crop (1 inter-row hoeing + 1 hilling up + manual weeding on the row); mechanical control (1 inter-row hoeing + 1 hilling up)]. Cover crops were sown in September and cut and ploughed just before potato planting in March. The potato crops following the cover crops were only fertilised with green manure. Averaged over years, all the cover crops produced more above-ground dry biomass than the weedy fallow (4.79 t ha⁻¹ on average vs 2.36 t ha⁻¹). Hairy vetch and subclover accumulated the highest N in the incorporated biomass (169 and 147 kg ha⁻¹), followed by snail medick (108), rapeseed (99), ryegrass (88) and weedy fallow (47). Rapeseed and ryegrass were the most efficient weed suppressors and had the least proportion of weed biomass (<1%) of the total produced by the cover, while they also reduced weed emergence in the following potato crops (8.8 plants m⁻²vs 25.5 plants m⁻² with all other cover crops). Following subclover and hairy vetch the potato crop yield was similar to that obtained by mineral N-P-K fertilisation (48.5 t ha⁻¹ of fresh marketable tubers). Mechanical weed control compared to weed free crop always reduced potato yield and the reduction, averaged over years, was greater in N-P-K mineral fertilised control (−23.6%) and smaller in ryegrass (−7.9%).     

Controlled reproduction in the wild European eel (Anguilla anguilla): two populations compared

This study aimed to describe the response variability of female silver eels in terms of gonad development and eggs production to a standardized gonadotropic treatment (Carp pituitary extracts—CPE), and to relate this variability to population characteristics. For this purpose, sexual maturation, ovulation, and fertilization were induced in two eel populations coming from different locations in Adriatic Sea (Comacchio—CM and Marano-Grado—MG lagoons), and after that, their reproductive capacity was valuated. External (Silver index—SI, Eye index—EI, Pectoral fin length index—PFLI, Condition factor—K) and hormonal (17β-estradiol—E2, testosterone—T) parameters were measured, and some subject/group were killed for histological and lipid analysis and age determination. Morphometric parameters showed the CM-Group to have highest values of Body weight (BW), Body length (BL), and K, while MG-Group presented highest PFLI and Gonadosomatic index (GSI) values. Regarding hormonal analysis, the CM-Group showed significantly higher T and E2 levels than the MG-Group, both groups showed considerably rapid increase at T5 (5th injection). A positive trend in gonadal development was found through histological evaluation; a more regular maturation was observed in the MG-Group, whereas the CM-Group presented an exponential oocytes development starting from T10 (10th week), which led to an anticipated spawning. Lipid content showed significant differences in T0 (start study), post-ovulation, and Control (30th week) between CM and MG eels. As to zootechnical performances, while MG eels released spontaneously into the water, the CMs were stripped in order to check ovulation. The MG eels were statistically the most productive with 40.1 ± 6.33 % BW of eggs released. Furthermore, CM females ovulated mainly between the 19th and 22nd week (77.8 % spawned eels) instead in the MG’s ovulation goes from the 24th to the 28th week (100 % spawned eels). As fertilization is of concern, in both groups fertilized eggs were obtained with no difference in larvae production. These results seem to indicate that bigger dimensions, higher K, and larger lipid content (Comacchio eels) could fasten gonadic maturation without positively influencing reproductive performance of animals, both in term of quantity and quality of produced eggs. Smaller females with a highest SI (Marano-Grado eels) presented a more regular gonadic development, leading the animals to spontaneous spawning.     

Preliminary Structure-Activity Relationship on Theonellasterol, a New Chemotype of FXR Antagonist, from the Marine Sponge Theonella swinhoei

Using theonellasterol as a novel FXR antagonist hit, we prepared a series of semi-synthetic derivatives in order to gain insight into the structural requirements for exhibiting antagonistic activity. These derivatives are characterized by modification at the exocyclic carbon-carbon double bond at C-4 and at the hydroxyl group at C-3 and were prepared from theonellasterol using simple reactions. Pharmacological investigation showed that the introduction of a hydroxyl group at C-4 as well as the oxidation at C-3 with or without concomitant modification at the exomethylene functionality preserve the ability of theonellasterol to inhibit FXR transactivation caused by CDCA. Docking analysis showed that the placement of these molecules in the FXR-LBD is well stabilized when on ring A functional groups, able to form hydrogen bonds and π interactions, are present.     

Isolation and Assessment of the in Vitro Anti-Tumor Activity of Smenothiazole A and B,Chlorinated Thiazole-Containing Peptide/Polyketides from the Caribbean Sponge,Smenospongia aurea

The study of the secondary metabolites contained in the organic extract of Caribbean sponge Smenospongia aurea led to the isolation of smenothiazole A (3) and B (4), hybrid peptide/polyketide compounds. Assays performed using four solid tumor cell lines showed that smenothiazoles exert a potent cytotoxic activity at nanomolar levels, with selectivity over ovarian cancer cells and a pro-apoptotic mechanism.