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101.
102.
Anna Andreetta Cristina Macci Maria Teresa Ceccherini Guia Cecchini Graziana Masciandaro Giacomo Pietramellara Stefano Carnicelli 《Biology and Fertility of Soils》2012,48(3):259-270
There is a growing interest in the links between humus forms and soil biota, and little is known about these links in Mediterranean
ecosystems. Culture-independent techniques, such as DNA extraction followed by DGGE and enzyme activities, allowed us to compare
microbial communities in two horizons of a forest soil in different seasonal conditions. Direct in situ lysis was applied
for extraction of DNA from soil; intracellular DNA was separated from extracellular and used to represent the composition
of microflora. The aims were to describe how biochemical and microbiological parameters correlate with topsoil properties
in typical Mediterranean Moder humus. Changes in bacterial and fungal community composition were evident from DGGE profiles.
Degrees of similarity and clustering correlation coefficients showed that the seasonal conditions may affect the composition
and activity of bacterial and fungal communities in the OH horizon, while in the E horizon the two communities were hardly
modified. In the same season, OH and E horizons showed a different composition of bacterial and fungal communities and different
enzyme activities, suggesting similar behaviour of eubacteria and fungi relatively to all the variables analysed. Evidently,
different organic carbon content in soil horizons influenced microflora composition and microbial activities involved in the
P and N cycles. 相似文献
103.
Eva Rioja Giacomo Gianotti Alexander Valverde 《The Canadian veterinary journal. La revue veterinaire canadienne》2013,54(9):864-868
Eight healthy dogs undergoing elective ovariohysterectomy were anesthetized with a standard protocol and received a low-dose medetomidine constant rate infusion during surgery. Cardiorespiratory parameters, including non-invasive cardiac output, were measured at various times. This protocol resulted in acceptable and stable cardiovascular performance, allowed low isoflurane concentrations, and provided smooth recoveries. 相似文献
104.
In traditional cattle systems in the tropics, the milk produced is generally shared between the calf and the cattle keeper. This literature review evaluates the socio-economic aspects related to milk production and milk use in traditional cattle systems and identifies the best strategies of milk allocation in order to improve food security and maximise income. The available literature indicates that milk, in terms of economic, social and subsistence value, is more valuable than meat. Thus, under the conditions that characterise traditional cattle systems in the tropics, it is appropriate to have a higher milk offtake at the expense of calf growth. This review also found that certain management practices, such as restricted suckling, can be useful to minimise mortality of calves, while improving milk offtake for human consumption. 相似文献
105.
Giacomo Rossi Chiara Bertani Subeide Mari Carlotta Marini Giacomo Renzoni Gregory Ogilvie Gian Enrico Magi 《Veterinary research communications》2013,37(2):101-108
Several studies of canine spontaneous mast cell tumours have described mutations in the c-kit proto-oncogene. These mutations produce a constitutively activated product and have been suggested to play a role in the malignant transformation of mast cells. We hypothesize that the selective tyrosine kinase inhibitor imatinib mesylate inhibits signal transduction and induces apoptosis when tested in cutaneous canine mast cell tumour samples positive for mutation in c-kit exon 11. Three-dimensional ex vivo cultures of canine grade II mast cell tumour treated with STI-571 at 48, 72, and 96 h and tested for signal transduction and apoptosis using appropriate assays were used. There was a progressive and significant increase in caspase-3 and TUNEL-positive mast cells compared to the untreated cultures. Additionally, a concurrent reduced expression of Ki67 and BCL-2 was observed. Furthermore, the treated cultures showed a marked reduction of Kit expression. Our results demonstrate that STI-571 induces Caspase-dependent apoptosis in a canine neoplastic mast cells possessing mutations in c-kit exon 11. 相似文献
106.
107.
A method was developed for estimating the number of surviving tobacco whitefly (TWF),Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae), nymphs after insecticide applications, based on the number of honeydew droplets ejected by TWF nymphs in 30 minutes. Honeydew droplets excreted by live TWF nymphs on a water-sensitive paper (Ciba-Geigy) stained blue and could easily be observed and counted. To estimate the number of live nymphs on an infested cotton leaf, the number of droplets ejected during 30 min was divided by a factor of 1.29. 相似文献
108.
Over 20 insecticides were screened against the tobacco whitefly,Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae), on cotton. AsB. tabaci feeds only on live plants, a suitable laboratory test method incorporating young cotton plants was developed. Both cover sprays and systemic insecticides applied to the soil were tested. Triazophos, pyridaphenthion, azinphos-methyl, bendiocarb and flucythrinate were found to be effective and persistent. The best of the systemic insecticides was aldicarb, followed by butocarboxim. 相似文献
109.
Maja J. Drozdzynska Yu-Mei Chang Giacomo Stanzani Ludovic Pelligand 《Veterinary anaesthesia and analgesia》2018,45(1):22-30
Objectives
Goal-directed fluid therapy (GDFT) based on pulse pressure variation (PPV) was used in anaesthetized dogs undergoing abdominal surgeries. The aims were 1) to evaluate the success rate of the PPV ≥13% in detecting fluid responsiveness [delta stroke volume (ΔSV) ≥10%]; 2) to assess the correlation between PPV, systolic pressure variation (SPV), Plethysmograph Variability Index (PVI) and central venous pressure (CVP) and 3) to establish the threshold value for the PVI that would predict a PPV value of ≥13% and indirectly discriminate responders from nonresponders to fluid therapy.Study design
Clinical, prospective, interventional study.Animals
A total of 63 client-owned dogs scheduled for abdominal procedures.Methods
PPV and SPV were calculated manually from the invasive blood pressure trace on the Datex monitor. PVI was recorded from the Masimo pulse oximeter. Fluid challenge (10 mL kg?1 Compound Sodium Lactate) was performed when PPV was ≥13% and/or mean arterial pressure (MAP) < 60 mmHg. Fluid responsiveness was assessed by the transoesophageal Doppler probe. Cardiovascular parameters (heart rate, MAP, PPV, SPV, PVI, SV and if available, CVP) were measured before and after each fluid intervention.Results
PPV ≥ 13% reliably predicted fluid responsiveness in 82.9% of cases. There was positive correlation between PPV and SPV (r = 0.84%), PPV and logPVI (r = 0.46) as well as SPV and logPVI (r = 0.45). Noninvasive PVI value ≥13% should predict PPV threshold value (13%) with 97% sensitivity and 68% specificity. There was no statistically significant correlation between PPV and CVP.Conclusions
PPV is a useful clinical tool to detect occult hypovolaemia and predict cardiovascular response to fluid challenge. Use of PPV is recommended as a part of GDFT in dogs undergoing abdominal procedures. 相似文献110.
Martina Fabbi Sabrina Manfredi Ezio Bianchi Giacomo Gnudi Francesca Miduri Antonella Volta 《The Canadian veterinary journal. La revue veterinaire canadienne》2016,57(4):382-386
A 12-year-old female dog with a 3-month history of poor response to diabetes treatment had an acute worsening of symptoms, including weakness and blindness. The dog had elevated blood glucose, alkaline phosphatase and urea concentration, hyposthenuria, glycosuria, hematuria, and pyuria. Escherichia coli was isolated from the urine. Radiographs and ultrasound examination showed that the dog had unilateral emphysematous pyelitis and concurrent cystitis associated with vesicoureteral reflux. 相似文献