用卡片PCR法检测卡耶他环孢子虫

使用FTA卡片法提取卡耶他环孢子虫DNA,利用特异性引物组F1E/R2B、CC719/CRP999进行巢式PCR,得到1条298bp的特异条带。并以柔嫩艾美尔球虫、贝氏隐孢子虫等作为对照,结果显示,除卡耶他环孢子虫外,均无特异性条带。用卡耶他环孢子虫卵囊进行巢式PCR敏感性试验,结果显示敏感度最高达1.0×100个/mL。     

深井离心泵的水力设计和二次回归正交试验

根据研发新型井泵节能节材的要求,将水力设计与计算流体动力学技术和二次回归正交试验方法相结合,进行提高井泵效率的试验研究.试验选择深井离心泵的叶轮出口安放角和出口宽度两个几何因素,按二次回归正交试验方案,设计了10副叶轮.通过计算流体动力学技术对包含叶轮、导叶在内的两级新型深井离心泵的全流场进行了设计工况下的三维流场数值模拟,得到了10组设计方案额定点的效率值.通过二次回归正交试验法研究了叶轮出口安放角、出口宽度对效率的影响规律,根据计算结果对新型深井离心泵效率提出了二次回归约束方程.结果表明,采用叶轮极大直径设计法对提高新型深井离心泵的水力效率具有一定的参考价值.     

Influence of Ammoniacal Copper Quaternary treatments on mechanical properties of blue-stained Lodgepole Pine wood

Three concentrations （2.8%, 2.0%, 1.2%） of Ammoniacal Copper Quaternary （ACQ） was selected to treat Lodgepole pine wood for evaluating ACQ treatment on mechanical properties of blue-stained wood. The bending modules of elasticity （MOE）, modules of rupture （MOR）, toughness and shearing strength parallel to grain on tangential surface, are tested according to the criteria GB1927-1943-91. Non-treated sample were also tested according to the same procedure. The results showed that the three groups specimen impregnated by different concentrations of ACQ solution met the AWPA standard 2003 of America （UC4A 6.4g/cm^3）. There were significant difference of toughness between treated wood and non-treated wood （p=0.01）, but there were no statistically significant differences among three concentrations in terms of toughness, and toughness of treated wood was approximately 20% lower than non-treated. MOR, MOE as well as sheafing strength parallel to grain were found to be not significantly different between treated wood and non-treated one, and there were no statistically significant difference among three concentrations of ACQ too. Toughness, MOR, MOE and sheafing strength parallel to grain increased with decrease of concentration of ACQ, but they were hardly affected by ACQ preservatives.     

沙棘木蠹蛾蛹的发育起点温度和有效积温研究

通过室外变温和室内恒温 2种不同的方法对沙棘木蠹蛾蛹的有效积温和发育起点温度进行了测定 ,结果表明 :变温条件下蛹的发育起点温度为 15.4 81°C,有效积温为 2 0 2 .356± 13.0 57日度 ;恒温条件下蛹的发育起点温度为 12 .0 55°C,有效积温为 2 95.2 0 3± 4 .0 94日度。蛹发育的最适温度为 2 1°C     

芥兰老化种子活力指标相关性分析

研究了芥兰种子经人工老化后各活力指标的相关性.结果表明,芥兰种子经人工老化后,各活力指标均有显著变化;种子发芽率,活力指数,POD活性与田间出苗率呈显著正相关,而种子浸出液的电导率则与田间出苗率呈显著负相关.经人工老化的芥兰种子发芽率与田间出苗率的相关程度最高,老化法发芽率可直接预测芥兰种子田间出苗率.     

黄河流域旱涝灾害的时空分布特征

本文以月降水距平为指标,根据黄河流域典型台站４０年的降水资料,对黄河流域旱涝灾害的时空分布特征进行了分析,并进行了区域划分。     

池塘循环流水养殖模式配套草鱼鱼种养殖试验

为完善池塘循环流水养殖模式,满足大规格鱼种的需求,选择草鱼为研究对象,开展了配套草鱼鱼种的池塘养殖试验。试验分设7000和10000尾/亩(15亩=1 hm^2,下同)两个放养密度,每个密度设3个平行组。经过190多天的养殖,高、低密度试验组水质变化趋势一致。低密度组鱼种平均体质量为239.67g/尾,成活率75.48%,净产量18 856 kg。以200 g/尾的规格挑选,共选留59 300尾,选留率74.83%;高密度组鱼种平均体质量为179.33 g/尾,成活率93.97%,净产量23 471 kg。以200 g/尾的规格挑选,共选留55260尾,选留率42.00%。结果表明,虽然高密度组平均成活率和净产量均高于低密度组,但低密度组的平均规格和选留率均远高于高密度组,最后的选留量也高于高密度组,因此,7 000尾/亩的放养密度更加适合于池塘循环流水养殖模式配套草鱼鱼种养殖。     

重庆城郊草莓生产现状调查分析

为全面了解重庆城郊草莓生产现状,更好地为重庆草莓科研和生产服务,通过问卷调查、现场走访和电话问询等形式,对重庆城郊草莓种植分布、品种、生产成本与效益、生产特点与市场价格及存在的问题等方面进行了调查分析.根据调查结果,指出了重庆草莓产业的发展方向.