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Objective To examine whether sub-optimal temperature induced stress and immunosuppression in farmed saltwater crocodile (Crocodylus porosus) hatchlings. Design A clinico-pathological study. Animals A total of 140 hatchlings were used. Procedure Body weight and length, plasma corticosterone and immunoglobulin concentrations and total and differential white blood cell counts were measured in 140 hatchlings from five clutches divided between five water temperature treatment groups. Initially all groups were housed at 32°C for 10 weeks, then two groups (L, LC) were changed to low temperature (28°C) and two groups (H, HC) to high temperature (36°C), while one group (C) remained at 32°C. The LC and HC groups were maintained at these temperatures for 10 days, after which the water temperature of both groups was returned to 32°C. Blood samples were collected twice (at 6 and 9 weeks of age) before the initial temperature change, and at 10 days and 4 weeks after the initial temperature change (at 11.5 and 14 weeks of age). Results Except for an increase in plasma corticosterone in the HC group and a decrease in the L group when the temperature change was first introduced, changes in plasma corticosterone were not significant. There were no significant changes in immunoglobulin concentrations. There were, however, significant decreases in the total white cell and lymphocyte counts in the LC group after the temperature was decreased to 28°C, and an increase in these counts after water temperature was returned to 32°C. Clutch of origin had significant effects on body weight and length gains, and there were negative relationships between body weight and corticosterone concentrations and between body weight and immunoglobulin concentrations. Conclusions As haematological changes indicative of stress were not associated with significant changes in serum corticosterone, immunosuppression in young crocodiles may be independent of the hypothalamic-pituitary-adrenal cortical axis. 相似文献
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We used 14C tracers to determine photosynthate distribution in cherrybark oak (Quercus pagoda Raf.) seedling sprouts following release from competing mid-story vegetation. Fall acquisition of labeled photosynthates by seedlings followed expected source-sink patterns, with root and basal stem tissues serving as the primary sinks. Four months after the seedlings had been labeled with 14C, they were clipped to induce sprouting. First-flush stem and leaf tissues of the resulting seedling sprouts were the primary sinks for labeled photosynthates stored in root tissues. Second-flush stem and leaf tissues, and first-flush stem and leaf tissues the following growing season, were not primary sinks for labeled photosynthates stored in root tissues despite the high radioactivity in root tissues. Root tissues appeared to deposit photosynthates in a layering process whereby the last photosynthates stored in new xylem were the first to be depleted during the initiation of a growth flush the following spring. There were more labeled photosynthates in roots of released seedling sprouts compared with non-released seedling sprouts, indicating increased vigor of released seedling sprouts in response to greater light availability. In contrast, stem and source leaf tissues of non-released seedling sprouts contained greater percentages of labeled photosynthates compared with released seedling sprouts, indicating either greater sink strength or poorly developed xylem and phloem pathways that created inefficiencies in distribution to root tissues. The 14C distribution coefficients confirmed the distribution patterns and provided additional information on the important sinks in released and non-released cherrybark oak seedling sprouts. 相似文献
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DJ STEWART JA VAUGHAN TC ELLEMAN† PA HOYNE† KJ BURNS JH DUFTY 《Australian veterinary journal》1991,68(2):50-53
The relationship between the serological classification system for serogroup B and for serogroup H of Bacteroides nodosus and cross-protection between subgroups within these serogroups was examined. Protection against ovine footrot following vaccination was achieved against other subgroup strains provided sufficient cross-reactive antibody was induced by shared pilus antigens. Within serogroup B, better cross-protection against one subgroup was obtained with a pili vaccine than a whole cell vaccine which correlated with higher pilus antibody titres induced by the former. For serogroup H, a lack of cross-protection and serological reactivity between subgroups was demonstrated, which indicates that the prototype strain of subgroup H2 should be designated a new serogroup. 相似文献
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