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991.
Dynamics in the membrane organization of the mammalian sperm cell and functionality in fertilization
B.M. Gadella F.M. Flesch L.M.G. van Golde B. Colenbrander 《The Veterinary quarterly》2013,33(4):142-146
Summary The capacitation process of sperm cells involves complex changes in the composition and orientation of molecules at the surface of the sperm cell. Here we focus on the lipid architecture in the sperm plasma membrane and demonstrate that the sperm plasma membrane is not static but is an extremely dynamic structure. Advanced fluoroscopic techniques enabled continuous monitoring of lipid organization in living cells and extremely rapid lipid movements were observed. The orientation of lipids in the sperm plasma membrane changed under capacitative treatments, was found to be sensitive for temperature and also changed upon binding of sperm cells to the zona pellucida. The changes in membrane properties coincided with an activation of protein kinases resulting in tyrosine phosphorylation of specific plasma membrane proteins. The detected membrane changes relate to intrinsic membrane properties such as fluidity, permeability, adhesiveness and fusibility. We think that these results may provide a physiological basis for new assays, able to discriminate between functional and non‐physiological sperm cells. 相似文献
992.
D I Bransby A G Matches C R Richardson 《African Journal of Range and Forage Science》2013,30(2):47-51
Abstract Due to rapid growth, stem build‐up in forage sorghum crops often leads to considerable wastage by animals and consequent loss of production. In this study the potential of the chemical plant growth regulator, mefluidide, for modifying the morphology of forage sorghum was examined. Mefluidide was applied to two consecutive plantings of forage sorghum at 0; 0,14 and 0,28 kg/ha. Treatment with mefluidide suppressed stem elongation of sorghum plants and stimulated tillering 4–6 weeks after application. After 6 weeks the stem:leaf ratio of treated plants was significantly lower (P ≤0,05) than that of control plants. On average, total dry matter (DM) yield was marginally higher for the control, but DM yield of leaf material was significantly higher. (P ≤0,05) for treated plots from 6 weeks after mefluidide application. No significant differences were observed between levels of mefluidide but responses of different variables over time differed between plantings. This was associated with different stages of plant growth when mefluidide was applied and different environmental conditions for two plantings. 相似文献
993.
Christian Swensson Gösta Gustafsson 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2013,63(2):49-56
The effects of manure handling system and feeding of dairy cows were studied using a simple method to analyse the level of ammonia release in cow stables. Two ratios based on the balances of ammonia and sensible heat, and ammonia and carbon dioxide, respectively, in animal houses were determined and considered to give characteristic levels of the release of ammonia in relation to the animal density independently of the ventilation rate. This required measurements of ammonia, carbon dioxide and temperature with hand-held instruments in the buildings and in outside air. In total, 34 dairy herds were investigated in the south of Sweden. The results clearly demonstrated higher release of ammonia from free stall barn with liquid manure than from conventional tie stall barn with solid manure. There was a clear effect of the content of crude protein, gram per kg dry matter, in the total feed ration on ammonia release in tie stall barn with liquid manure. 相似文献
994.
995.
N. G. Sales S. A. dos Santos F. P. Arantes R. E. S. Hojo J. E. dos Santos 《Anatomia, histologia, embryologia》2013,42(3):213-219
The morphology of the ovaries and oogenesis of Pimelodella vittata were studied using anatomical and histological techniques to provide information of its reproductive biology. Eighty adult females were captured trimonthly during the period November 2005 to October 2006. The ovaries are paired, saculiform organs, which are coated with tunica albuginea and contain ovigerous lamellae, where the oocytes develop before being released into the ovarian lumen and following the ovarian duct until reaching the genital papilla. Oogenesis was divided into stages based on the alterations to the nucleus, ooplasm and surrounding follicular layers. Oogonia form groups from the germinal epithelium have asynchronous development and differentiate into initial perinucleolar oocytes. The formation of the zona pellucida is initiated in the advanced perinucleolar oocytes reaching a thickness of 1.46 ± 0.58 μm in the vitellogenic oocytes. The follicular cells are squamous in perinucleolar oocytes, become cubical in the pre‐vitellogenic oocytes and prismatic in the vitellogenic oocytes with a height of 11.20 ± 4.74 μm. The histochemical reactions indicate that zona pellucida, cortical alveoli and yolk globules contain neutral glycoproteins and the follicular cells contain neutral glycoproteins in association with carboxylated and sulphated glycoconjugates. Statistical analyses showed significant differences in the diameter of the oocytes and follicular cells height as oocytes matured. This study represents the first data about the ovarian structure and oogenesis of this species. 相似文献
996.
Luis A. Saavedra-Jiménez Rodolfo Ramírez-Valverde Rafael Núñez-Domínguez José G. García-Muñiz Nicolas Lopez-Villalobos Agustín Ruíz-Flores 《Tropical animal health and production》2013,45(7):1489-1494
The objective of this study was to determine the magnitude of genotype by climate interaction (GCI) in the national genetic evaluation for weaning (WW) and yearling (YW) weights of Mexican Braunvieh cattle. The numbers of performance records and animals in the pedigree were 12,364 and 25,173 for WW, and 7,991 and 18,072 for YW, respectively. Performance records were clustered based on climatological variables into: dry tropic (DT), wet tropic (WT), and temperate (TE) climates. Animal models were used to estimate genetic parameters and predict breeding values in each of the climates. Bivariate analyses were carried out for pairwise combinations of climates on each trait, considering the same trait in different climates as a different trait. Criteria to evaluate GCI were genetic correlations (r g), correlations between predicted breeding values (r BV), and frequencies of coincidence (FC) in the ranking of the top 25 sires. Results of comparisons between pairs of climates were variable, depending on specific cases. For WW, the r g, r BV, and FC ranged from ?0.36 to 0.84, ?0.60 to 0.97, and 0.16 to 0.92, respectively; whereas for YW, they fluctuated between 0.23 and 0.99, 0.33 and 1.00, and 0.60 and 1.00, respectively. For both traits, the results suggest absence of GCI between DT and TE; however, GCI was detected in the other pairs of climates, where WT was involved. To maximize genetic progress, the joint genetic evaluation should be performed only for animals with performance data in DT and TE, whereas a separated evaluation is suggested for animals with performance records generated under WT conditions. 相似文献
997.
Neis C Rohde J Valentin-Weigand P Baums CG 《Berliner und Münchener tier?rztliche Wochenschrift》2007,120(5-6):202-206
Streptococcus (S.) suis plays an important role in pig breeding causing invasive diseases such as meningitis and polyarthritis. Herd problems with Streptococcus suis are common in many pig farms and are frequently characterised by disease outbreaks. In this context, it is often important to identify chains of infection, e.g. between a farrow-to-wean and a grower farm. In the following case report a possible chain of infection among the different farms of a farrow-to-finish system was investigated. In two grower units herd problems with S. suis were present; the mortality was higher than 5 %. It appeared likely, that the streptococci causing these problems were transmitted from a single farrow-to-wean unit to the two different grower farms. In the respective farms swabs were taken from different healthy animals and, in the case of the grower farms, also from the infected animals. Genotypic profiling of strains by a multiplex-PCR and AFLP typing method revealed that two different S. suis pathotypes were responsible for the herd problems. Both pathotypes could not be detected in the farrow-to-wean farm.Thus, a chain of infection originating from the farrow-to-wean farm appeared unlikely. The multiplex PCR was in this case sufficient to elucidate the described problem. 相似文献
998.
999.
Relationship between the serum concentrations of serotonin and lipids and aggression in dogs 总被引:1,自引:0,他引:1
The serum concentrations of serotonin and lipids--triglycerides, total cholesterol, low density lipoprotein, high density lipoprotein and very low density lipoprotein--were determined in 18 normal dogs and 23 dogs diagnosed as aggressive on the basis of interviews with their owners and an assessment of their behaviour with reference to a canine overt aggression chart. The serum serotonin levels in the aggressive dogs were significantly lower than in the normal dogs (P<0.01), but the differences in serum lipids between the two groups were not statistically significant. 相似文献
1000.
Arunvipas P Vanleeuwen JA Dohoo IR Leger ER Keefe GP Burton AS Lissemore KD 《Preventive veterinary medicine》2007,82(1-2):42-50
Our objective of this study was to investigate the relationship between milk urea-nitrogen concentrations ([MUN]) and first-service breeding success (FSBS) in a large number of commercial dairy herds, using various timings on [MUN]. All commercial dairy herds in Prince Edward Island on monthly milk testing (n=198) formed the sampling frame. Milk components, [MUN], 24-h milk production, and breeding data for all cows from these farms were gathered electronically from a central database. A first service between 1 June 1999 and 31 May 2000 was classified successful (FSBS=1) if it was the cow's last service and she calved 270-290 d later. Mixed logistic-regression modeling was used to determine the association between FSBS (the outcome variable) and the [MUN] closest to first service, controlling for other possible confounders and clustering effects of cows within the study herds. The final dataset included 2787 successful and 3015 unsuccessful first services. A change in [MUN] on the test closest to first service from 10 to 20 mg/dL was associated with a 13.9% reduction in the odds of FSBS (controlled for parity, milk production and days in milk). 相似文献