湖南野生兰花的调查研究 Ⅱ.野生春兰茎的生物学特性

在对野生兰花形态特征观测的基础上，进一步探讨了不同根茎和假鳞茎的产生和生长发育的特点，根茎与假鳞茎的关系及有关的规律。     

新疆博尔塔拉河干流段水量入,出关系分析

本研究运用线性系统的理论和单、双序列的频谱分析方法，分析了新疆博尔塔拉河干流段温泉站入流量、博乐站出流量的自谱特征和互谱关系。文中提出的几点结论可供流域水资源计算及评价参考     

关中地区温室越冬茬番茄黄化曲叶病毒病分子鉴定及流行规律

为了明确关中地区越冬茬番茄黄化曲叶病毒病发生和流行规律,通过分析该病发生与番茄品种、定植期及传播介体烟粉虱之间的关系,并采用PCR技术对田间病原进行分子鉴定。结果表明,番茄黄化曲叶病毒病在8月中下旬至11月上中旬开始侵染,翌年3月中下旬发生再侵染,秋季病情减轻;烟粉虱种群数量与病害发生程度呈线性正相关;不同番茄品种对番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)的抗性差异显著,其中大番茄品种布鲁尼1288和DRW7728,小番茄品种千禧和美红对该病表现为免疫;分子检测结果表明,4个样品中均扩增出543 bp的特异片段,与NCBI数据库Gen Bank的TYLCV序列(登录号为GU084381、KC138544.1、KC138543.1和JX456642.1)的相似性达99%。研究表明,关中地区番茄病毒病为番茄黄化曲叶病毒病,番茄品种、定植期及烟粉虱发生动态是影响该病发生的主要因素。     

强化兽药GSP后续监管 保障兽药产品质量安全

随着兽药GSP认证工作的全面结束,兽药GSP的日常监管工作越来越凸显其重要性。本文综合论述了在兽药GSP日常监管过程中发现的诸多问题,以及今后如何做好兽药GSP后续监管工作所采取的措施。     

High Throughput Sequencing to Study the Effect of Chronic Renal Failure on the Diversity and Gene Function Prediction of Gut Microbiota in Dogs

This study aimed to investigate the effect of chronic renal failure(CRF) on the gut microbiota diversity and predict the gene function of the flora. A total of 30 2-year-old dogs were selected and randomly divided into the chronic renal failure model group (CRF, established by renal artery ligation), sham operation control group (Sham) and healthy control group (HCG). All animals were fed normally during the 56 days of test period. The serum creatinine (Scr), blood urea nitrogen (BUN) and urine protein / creatinine ratio (UP/C) were detected regularly during the experiment. The effects of chronic renal failure on the structure, diversity and function of gut microbiota were analyzed according to the result of bacterial 16S rDNA sequencing from fresh feces collected without contamination. The flora markers index (FMI) was constructed based on the principal component Logistic regression analysis of different microbiota in CRF group to predict the development of chronic renal failure. The results showed that: 1) The levels of Scr, BUN and UP/C in CRF group from the start of the 28th day of the experiment were significantly higher than those of HCG group and Sham group (P<0.05), and significantly higher than that of the first day of CRF group (P<0.05). 2) Compared with those before chronic renal failure (CRF group at day 0 and 28), HCG group and Sham group, the Chao 1 diversity and Shannon diversity of gut microbiota in CRF group at day 56 were significantly lower (P<0.05), while the relative abundance of Bacteroidetes, Proteobacteria and Actinobacteria significantly increased and the number of Firmicutes significantly decreased (P<0.05). 3) LEfSe analysis showed that 20 species were enriched in CRF group, mainly including Pseudomonas, Escherichia, Proteus and so on, and most of them had negative correlation with other intestinal bacteria. Functional prediction revealed that genes of those different species were mainly enriched in amino acid metabolism, sugar biosynthesis and metabolism and carbohydrate metabolism in CRF group. The area under ROC curve (AUC) of the FMI constructed with those species enriched was 0.788, which could be used as the intestinal microbial marker for CRF in dogs. In summary, chronic renal failure can reduce the diversity of intestinal microbiota, lead to the imbalance of bacterial structure and change of bacterial function. Moreover, the enriched gut microbiota in CRF group can be used as the intestinal microbial marker of CRF in dogs, and the best prediction effect can be obtained by FMI.     

澳大利亚动物疫病防控体系简介

澳大利亚动物疫病净化工作有许多值得借鉴之处。本文就澳大利亚畜牧业基本情况、兽医服务体系、动物标识和疫病追溯体系和动物疫病应急管理体系等方面进行了介绍,列举了7个方面的可借鉴之处,包括联邦政府和各州政府动物疫病防控分工明确、各级兽医服务专业化程度高、行业协会在动物疫病控制中地位显著、动物标识体系完善、动物疫病控制规划详尽、疫病净化政策坚持不懈和兽医流行病学技术应用纯熟等,以期对我国动物疫病防控体系建设工作有所启发。     

膨化菜籽对肉鸡生产性能及部分血液生化指标的影响

试验选用160只21日龄的艾维茵肉鸡,随机分为5组,每组4个重复(栏),每栏8只鸡,在玉米-豆粕型日粮中添加不同水平膨化菜籽(2.0%、6.0%、10%和14%)。试验结果表明:(1)整个生长期膨化菜籽组与对照组相比,平均日增重、日采食量均有显著提高(P<0.05),料重比显著降低(P<0.05),添加6.0%膨化菜籽组对肉鸡促生长效果最好;(2)膨化菜籽组与对照组相比,对肉鸡血清中白蛋白、尿素氮、碱性磷酸酶、总胆固醇和甘油三酯等生化指标没有显著性影响(P>0.05)。     

诱杀球对橘大实蝇诱捕效果的研究

为开发一种新型的橘大实蝇诱捕器,研究了不同颜色、直径的诱杀球和不同颜色的粘虫板对橘大实蝇的诱集效果。结果表明,绿色诱杀球的诱杀数量显著大于黄色和黄绿色诱杀球(P0.05);诱杀球上橘大实蝇的诱集数量,随诱杀球直径的增大而显著增加,但在直径7cm以上时,诱杀球的诱集数量增速变缓,其中以直径7cm的绿色诱杀球生产成本和诱集效果最佳;诱杀球能显著提高黄板对橘大实蝇的诱集效果(P0.05)。