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141.
Wu Jian et al. 《安徽农学通报》2008,(11)
本试验通过开顶式气室控制CO2含量,研究了大气CO2增长对水稻籽粒蛋白质及氨基酸组分含量的影响。结果表明,在大气CO2含量(摩尔分数)为550和750μmol.mol-1时,与正常大气CO2水平相比,水稻籽粒蛋白质含量和氨基酸总量有下降趋势,蛋白质含量呈下降趋势,分别下降3.10%和5.49%,氨基酸中的色氨酸和组氨酸下降幅度最大,分别为17.19%-35.16%和10.81%-28.38%。 相似文献
142.
143.
Effects of diatom concentration in prepared feeds on growth and energy budget of the sea cucumber Apostichopus japonicus (Selenka) 下载免费PDF全文
Ce Shi Shuanglin Dong Surui Pei Fang Wang Xiangli Tian Qinfeng Gao 《Aquaculture Research》2015,46(3):609-617
Eight test diets were used in the experiment: seven fresh diatom (Cylindrotheca fusiformis) diets in which diatom occupied 100%, 83.3%, 50%, 33.3%, 20%, 14.3% and 11.1% in dry matter of the feeds (marked as D100, D83, D50, D33, D20, D14 and D11), and a formulated diet (70% Sargassum thunbergii powder, 20% sea mud and 10% fish meal, based on dry weight, marked as ST). The results showed that the specific growth rates (SGRs) of the animals fed diets D33, D20, D14 and D11 were not significantly different from the SGR of those fed diet ST. However, the energy allocated to growth for the animals fed with diet D14 was twice that of diet ST. For sea cucumbers fed diet D14 the largest expenditure part was allocated to respiration (55.4%), but for those fed diet ST, the largest part was allocated to faeces (62.7%). The organic content of diet D14 was also much lower than that of diet ST. Frozen diatom diets containing 14% (dry matter weight) fresh diatom could be an environmentally friendly feed as an alternative option for macroalgae powder. 相似文献
144.
This study was conducted to investigate the effects of compound p1ant nutrients (CPN) on rumen fermentation parameters, rumen microbiota and fatty acid composition of longissimus dorsi musc1e in finishing sma11 tai1 Han sheep. Sixteen 4-month-o1d finishing sma11 tai1 Han sheep with an initia1 body weight (BW) of (24.18±0.31) kg were random1y divided into two groups, name1y, with 8 rep1icates per group and 1 sheep per rep1icate. The sheep in the contro1 group were fed a basa1 diet, whereas the sheep of the contro1 group (CPN group) was fed the basa1 diet supp1ementation with 3‰ CPN. The experiment 1asted for 97 days after 7 days adaption. The resu1ts showed as fo11ows: compared with the contro1 group, 1) adding CPN decreased the concentration of ammonia nitrogen (NH3-N) (P<0.05); 2) CPN supp1ementation affected beta diversity of rumen microbiota; 3) the re1ative abundance of Acidobacteriota, Erysipe1otrichaceae_UCG-002, Lactobacillus, and Megasphaera were enhanced by adding CPN (P<0.05), whereas, the re1ative abundance of Bacteroidetes and Prevotella was 1ower (P<0.05); 4) the supp1ementation of CPN had no significant effect on fatty acid composition of longissimus dorsi musc1e of fattening sma11-tai1ed Han sheep (P>0.05); 5) the contents of tota1 MUFA, C18:1n9c, C14:1, C16:1, C18:2n6c, C18:3n3 and n-3PUFA in the longissimus dorsi musc1e were corre1ated with the re1ative abundance of Megasphaera, Erysipe1otrichaceae_UCG-002, Succiniclasticum and Ruminococcus (P<0.05). In conc1usion, CPN can regu1ate the rumen microbiota structure and reduce the rumen NH3-N concentration of fattening sma11-tai1ed Han sheep. In production practice, CPN can be used as a rumen eco1ogica1 regu1ator. © 2023 Authors. All rights reserved. 相似文献
145.
146.
【研究目的】应用SOTER数据库与RUSLE模型定量化估算河北省土壤侵蚀风险。【方法】在建立河北省SOTER数据库的基础上,提取地形因子、植被覆盖与耕作管理措施和土壤保持措施,结合土壤可蚀性与降雨侵蚀力因子,应用RUSLE模型定量评价河北省山区土壤侵蚀风险。【结果】河北省轻度水土流失面积3.5万km2,占18.9%;中度水土流失面积1.1万km2,占6.1%;强度与极强度水土流失面积分别为8 183km2与1.0万km2,分别占全省面积的4.4%和5.6%;剧烈土壤侵蚀面积为1.2万km2,占全省面积的6.8%。【结论】河北省土壤侵蚀主要发生在太行山、燕山山区,尤以低山丘陵地带的土石山区最为严重。 相似文献
147.
Effects of dietary lysozyme levels on growth performance,intestinal morphology,non‐specific immunity and mRNA expression in weanling piglets 下载免费PDF全文
Jiatao Zhu Xiaoxue Pang Zhengfeng Fang Yan Lin Lianqiang Che Shengyu Xu Jian Li Yiming Huang Xiang Su De Wu 《Animal Science Journal》2016,87(3):411-418
The aim of the present study was to determine the effect of dietary lysozyme levels on growth performance, gut health and non‐specific immunity of weanling piglets. A total of 150 weanling piglets were allocated to six treatments. The piglets were fed the same basel diet supplemented with 0, 30, 60, 90 and 120 mg/kg lysozyme as well as antibiotics for 28 days. From day 14 to day 28 of dietary treatment, piglets fed 90 mg/kg lysozyme had greater average daily gain than piglets fed control diet. During the whole experimental period, piglets fed 120 mg/kg lysozyme tended to have greater average daily gain than piglets fed control diet. Compared with piglets fed control diet, piglets fed diets containing antibiotics and 90 mg/kg lysozyme had greater villus height to crypt depth ratio in duodenum and jejunum. Additionally, dietary supplementation of 60 and 90 mg/kg lysozyme as well as antibiotics enhanced the phagocytic activity of peritoneal macrophages in piglets. In conclusion, dietary lysozyme can accelerate the growth of weanling piglets by improving gut health and non‐specific immunity and supplementing 90 mg/kg lysozyme is as effective as antibiotics (20 mg/kg colistin sulphate + 50 mg/kg kitasamycin) in improving the growth performance of weanling piglets. 相似文献
148.
猪Ghrelin基因的克隆及原核表达 总被引:1,自引:0,他引:1
从猪下丘脑、胃等组织中提取总RNA,根据已发表的猪的Ghrelin mRNA序列设计合成引物,通过RT-PCR进行cDNA扩增,获得了282bp的片段。将该片段克隆于pMD-18T载体后进行序列分析,确认PCR产物为Ghrelin cDNA。从阳性克隆中提取质粒,经NheⅠ和XhoⅠ双酶切,回收282bp的目的片段,定向克隆到pET-28a表达载体中,提取质粒并再次转化到BL21(DE3)中,成功地筛选出阳性克隆。经IPTG诱导阳性菌,通过SDS-PAGE检测出猪Ghrelin基因的表达. 相似文献
149.
AIM: To investigate the role of miR-181b in the expression of Bcl-2 and SP1 at mRNA and protein levels in the human hepatoma G2 cells (HepG2), and to explore the effect of miR-181b on the regulation of HepG2 cell proliferation. METHODS: The synthetic double-strand complementary DNA based on the sequence of miR-181b was inserted into the vector of miRNASelectTM pEGP-miR. The microRNA high-expression plasmid was cloned, and the sequences were identified. The miR-181b plasmid was transfected into HepG2 cells with liposomes. The stable cell line was screened by puromycin. The mRNA and protein levels of Bcl-2 and SP1 were measured by RT-PCR and Western blotting, respectively. Methyl thiazolyl tetrazolium (MTT) method was used to analyze the proliferation of HepG2 cells. RESULTS: The Western blotting results showed that miR-181b inhibited the protein expression of Bcl-2 and SP1. The result of RT-PCR also indicated that the mRNA expression of Bcl-2 and SP1 was suppressed. Compared with the control, the growth rate of HepG2 with high expression of miR-181b was significantly decreased.CONCLUSION: miR-181b inhibits the proliferation of HepG2, which may be related to the down-regulation of Bcl-2 and SP1. 相似文献
150.
马蹄金是一种重要的地被植物,然而目前利用的种子依赖于进口,如何减少种子利用是生产中面临的问题。试验以马蹄金作为材料,按10、13、16、19、22g/m2进行播种,采用随机区组设计,通过相对生长速率、盖度、密度及草坪综合质量等指标测定,筛选出适宜亚热带地区的播种量。结果表明:播种量为19g/m2的条件下,生长80d后马蹄金草坪密度达到165.57株/米2,盖度为69.78%;与其他处理相比,播种量为19g/m2杂草发生率少,且盖度增加快,均一性好,综合质量最高(P0.05)。因而,19g/m2可以作为马蹄金在亚热带地区建植草坪时的适宜播种量。 相似文献