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Y. J. Huang C. Toscano-Underwood B. D. L. Fitt † X. J. Hu A. M. Hall 《Plant pathology》2003,52(2):245-255
Ascospores of both A-group and B-group Leptosphaeria maculans germinated at temperatures from 5 to 20°C on leaves of oilseed rape. Germination of ascospores of both groups started 2 h after inoculation and percentage germination reached its maximum about 14 h after inoculation at all temperatures. Both the percentage of A-/B-group ascospores that had germinated after 24 h incubation and germ tube length increased with increasing temperature from 5 to 20°C. Germ tubes from B-group ascospores were longer than those from A-group ascospores at all temperatures, with the greatest difference at 20°C. Hyphae from ascospores of both groups penetrated the leaves predominantly through stomata, at temperatures from 5 to 20°C. A-group ascospores produced highly branched hyphae that grew tortuously, whereas B-group ascospores produced long, straight hyphae. The percentage of germinated ascospores that penetrated stomata increased with increasing temperature from 5 to 20°C and was greater for A-group than for B-group L. maculans after 40 h incubation. 相似文献
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中生菌素对水稻白叶枯病的防治机制 总被引:6,自引:1,他引:6
对不同白叶枯病抗性水稻品种用200μg/ml中生菌素55℃温汤药液浸种,自然降温,秧田3—4叶期和移栽前5d各用30μg/ml中生菌素处理后,于成株期剪叶接种白叶枯病菌。结果表明中生菌素前期处理,于成株期接种白叶枯病菌时,高抗、中等抗性和感病品种中过氧化物酶、苯丙氨酸解氨酶和多酚氧化酶3种酶活性都有不同程度的提高,且以中等抗性品种酶活提高最多,接种24和48h,3种酶活性较清水对照分别增加26.92%、26.74%、24.06%和7.09%、1.31%、1.60%。盆栽试验表明,中生菌素对中抗品种的防治效果最好,达58.4%。说明中生菌素对水稻防御酶活性的诱激作用是其防治白叶枯病的机制之一。 相似文献
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20世纪80年代后,稻瘿蚊发生危害明显回升,损失相当严重。以大田试验示范为主,系统研究了新耕作条件下稻瘿蚊综合防治,首次总结提出“喷药除草、压低虫源、抓住晚秧、做好测报、挑治本田”的综合防治新技术体系。室内外试验和大田示范表明,应用草甘膦杀死越冬寄主游草,可显著减少虫源基数;灭线磷(商品名益舒宝)、氯唑磷(商品名米乐尔)是防治稻瘿蚊的高效药剂,抗蚊青占品种高抗稻瘿蚊。此外,合理利用杀虫植物、性引诱剂和天敌等亦是防治稻瘿蚊的重要措施。在面积达12.46万hm^2的示范推广实践中,本田防治效果均达80%以上。 相似文献
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N. J. Grünwald F. Workneh S. Hu A. H. C. van Bruggen 《European journal of plant pathology / European Foundation for Plant Pathology》1997,103(1):55-63
Testing of soil samples in greenhouse assays for suppressiveness to soilborne plant pathogens requires a considerable investment in time and effort as well as large numbers of soil samples. To make it possible to process large numbers of samples efficiently, we compared an in vitro growth assay with a damping-off assay using Pythium aphanidermatum as the test organism on tomato seedlings. The in vitro test compares the radial growth or relative growth of the fungus in soil to that in autoclaved soil and reflects suppressiveness of soils to the pathogen. We used soils from a field experiment that had been farmed either organically or conventionally and into which a cover crop (oats and vetch in mixture) had been incorporated 0, 10, 21, and 35 days previously. We obtained a significant, positive correlation between damping-off severities of tomato seedlings in damping-off assays and both relative and radial growth in vitro. In addition, radial and relative growth of P. aphanidermatum in the in vitro assay were positively correlated with several carbon and nitrogen variables measured for soil and incorporated debris. We did not find differences between the two farming systems for either growth measures of P. aphanidermatum or disease severities on tomato at different stages of cover crop decomposition. The in vitro assay shows potential for use with any fungus that exhibits rapid saprophytic growth, and is most suitable for routine application in suppressiveness testing. 相似文献