Indirect immunofluorescence and immunodiffusion tests in the detection of antibodies to foot-and-mouth disease virus

The antibody response detected by indirect immunofluorescence (IIF) as well as that directed against 140 S and virus infection associated antigen (VIA), as detected by agar immunodiffusion, was studied in three mammal species susceptible to Foot and Mouth Disease Virus, after challenge with living virus, immunization and hyperimmunization with inactivated virus, and immunization followed by challenge. By spot indirect immunofluorescence, antibodies were detected only in animals undergoing an active infection, and were not detected in immunized or hyperimmunized animals. This behaviour was similar to that of the anti-VIA antibodies in the same groups of animals and differed from that of anti-140 S antibodies. It appeared that spot indirect immunofluorescence for the detection of VIA antigen is comparable to the immunodiffusion test, but the speed of IIF and the possibility of handling many samples make it more practical.     

Use of the Single Seed Descent Method in Breeding Safflower (Carthamus tinctorius L.)

The single seed descent (SSD) method of selection has been evaluated in safflower (Carthamus tinctorius L.). The initial population of this study was a BC₂F₁ from a cross between a well adapted and a high oil cultivar. Transgressive segregation for yield and oil content producing lines with considerably higher values than the better parent was observed in BC₂F₄ and BC₂F₅ generations. These results and the great saving of time and effort makes the SSD method a very desirable tool for safflower breeders.     

Assessment of the Extent to Which Intensively-studied Lakes are Representative of the Adirondack Region and Response to Future Changes in Acidic Deposition

Many lakes in the Adirondack Mountains, New York, have acidified over the past century due to acidic atmospheric deposition. More recently, most monitored lakes have shown signs of chemical recovery (increase in acid neutralizing capacity) as sulfur deposition levels have declined in response to the Clean Air Act and other emissions control legislation. We used measured and modeled trends in past lakewater acidification and projections of future recovery from acidification to extrapolate results from judgment samples of intensively studied lakes to the population of acid-sensitive Adirondack lakes. Simulations were developed for 70 watersheds using the Model of Acidification of Groundwater in Catchments (MAGIC) to classify lakes according to their sensitivity to change in atmospheric S and N deposition. MAGIC simulations suggested that the modeled Adirondack Long-Term Monitoring Project (ALTM) and Adirondack Effects Assessment Project (AEAP) lakes were largely among the lakes in the population that had acidified most between 1850 and 1990. Most of the modeled ALTM/AEAP lakes were within the top 36% of acid sensitivity, based on model projections of past acidification and future chemical recovery, compared with the 1,829 Adirondack lakes in EPA’s Environmental Monitoring and Assessment Program (EMAP) statistical frame. Results of this research will allow fuller utilization of data from on-going chemical and biological monitoring and process-level studies by providing a basis for regionalization of findings and developing/refining relationships among watershed characteristics, chemical change, and biological responses to changing levels of acidic deposition.     

Effects of input management and crop diversity on non-renewable energy use efficiency of cropping systems in the Canadian Prairie

Although producers’ prime objective may be to increase net returns, many are also interested in conserving and enhancing the quality the soil, water and air resources through adopting more environmentally friendly production practices. This study compared non-renewable energy inputs, energy output, and energy use efficiency of nine dryland cropping systems comprised of a factorial combination of three methods of input management [high (HIGH), i.e., conventional tillage plus full recommended rates of fertilizer and pesticides; reduced (RED), i.e., conservation tillage plus reduced rates of fertilizer and pesticides; and organic (ORG), i.e., conventional tillage plus N-fixing legumes and non-chemical means of weed and pest control]; and three crop rotation systems with varying levels of cropping diversity [a fallow-based rotation with low crop diversity (LOW); a diversified rotation using annual cereal, oilseed and pulse grains (DAG); and a diversified rotation using annual grains and perennial forages (DAP)]. The study was conducted over the 1996–2007 period on a Dark Brown Chernozemic soil (Typic Boroll) in the Canadian Prairies. As expected, total direct plus indirect energy input was the highest for the HIGH and RED input treatments (3773 MJ ha^?1 year^?1), and 50% less for ORG management. Most of the energy savings came from the non-use of inorganic fertilizers and pesticides in the ORG management treatments. Further, total energy use was the highest for the DAG treatments (3572 MJ ha^?1 year^?1), and similar but about 18% lower for the DAP and LOW crop diversity treatments compared to DAG. Thus, overall, the HIGH/DAG and RED/DAG systems had the highest energy requirements (4409 MJ ha^?1 year^?1) and ORG/DAP had the lowest (1806 MJ ha^?1 year^?1). Energy output (calorimetric energy content) was typically the highest for the HIGH input treatments (26,541 MJ ha^?1 year^?1), was about 4% less with RED, and 37% less with ORG management. The latter reflected the lower crop yields obtained with organic management. Similarly, energy output was the highest for the DAP treatments (25,008 MJ ha^?1 year^?1), about 5% less for DAG, and 20% less for the LOW crop diversity treatments. The higher energy output with the DAP treatments largely reflected that the entire harvested biomass of the forage crops was included in energy output, while for grain crops only the seed was included. The straw and crop residues from annual crops were returned to the land to protect the soil from erosion and to maintain soil organic matter as this is the recommended practice in this semi-arid region. In contrast to energy output and to net energy produced, energy use efficiency (measured as yield of grain plus forage produced per unit of energy input or as energy output/energy input ratio) was the highest for the ORG input treatments (497 kg of harvested production GJ^?1 of energy input, and an energy output/energy input ratio of 8.8). We obtained lower, but generally similar energy use efficiency for the HIGH and RED input treatments (392 kg GJ^?1 and ratio of 7.1). Thus, overall, ORG/DAP was the most energy efficient cropping system, while RED/LOW and RED/DAG generally ranked the lowest in energy use efficiency. Our findings support the current movement of producers toward ORG management as a means of reducing the reliance on non-renewable energy inputs and improving overall energy use efficiency of their cropping systems. Our results also suggest that moving away from traditional monoculture cereal rotations that employ frequent summer fallowing, toward extended and diversified crop rotations that use reduced tillage methods, although resulting in an increase in energy output, will not significantly reduce the overall reliance on non-renewable energy inputs, nor enhance energy use efficiency, unless perennial legume forages and/or legume grain crops are included in the cropping mix.     

Microspectrophotometry of photoreceptor organelles from eyes of the prawn Palaemonetes

Microspectrophotometric measurements of individual dark-adapted rhabdoms of the prawn Palaemonetes vulgaris reveal the presence of two light-sensitive pigments. A pigment with maximum absorbancy at 555 nanometers is converted by light to a long-lived intermediate with wavelength of maximum absorbancy at 496 nanometers. A second pigment with wavelength of maximum absorbancy at 496 nanometers bleaches in the light, seemingly without forming detectable products at wavelengths longer than 375 nanometers. Both pigments occur in each layer of microvilli.     

Role of nonimmune IgG bound to PfEMP1 in placental malaria

Infections with Plasmodium falciparum during pregnancy lead to the accumulation of parasitized red blood cells (infected erythrocytes, IEs) in the placenta. IEs of P. falciparum isolates that infect the human placenta were found to bind immunoglobulin G (IgG). A strain of P. falciparum cloned for IgG binding adhered massively to placental syncytiotrophoblasts in a pattern similar to that of natural infections. Adherence was inhibited by IgG-binding proteins, but not by glycosaminoglycans or enzymatic digestion of chondroitin sulfate A or hyaluronic acid. Normal, nonimmune IgG that is bound to a duffy binding-like domain beta of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) might at the IE surface act as a bridge to neonatal Fc receptors of the placenta.     

Isoflavonoids isolated from Cuban propolis

Chemical investigation of a red-type Cuban propolis sample has led to the isolation of 11 isoflavonoids (2 isoflavones, 3 isoflavans, and 6 pterocarpans), together with gallic acid, isoliquiritigenin, and (-)-liquiritigenin. Structural determination, including the absolute stereochemistry, was accomplished by spectroscopic analysis, particularly CD and 2D NMR techniques. The fragmentation behavior of pterocarpans was studied by electrospray ionization (ESI) tandem mass spectrometry (MS/MS) using an ion-trap analyzer, and a generalized fragmentation pathway, useful in the identification and structural characterization of pterocarpans, is proposed. Isoflavonoids are reported for the first time from propolis samples.     

Temporal and Spatial Monitoring of the pH and Heavy Metals in a Soil Polluted by Mine Spill. Post Cleaning Effects

The bursting of the mining dam of Aznalcollar (Seville, Spain) triggered an increase in the concentration of heavy metals in the soils of the river Guadiamar valley as a result of the leaching of the pyritic sludge deposited on them. After the cleaning operations which included, as well as mechanical clearing, the addition of different amendments, some areas with residual sludge remained, from which some heavy metals are being mobilized by the cyclical recharge and discharge processes of water in the profiles. This paper analyzes the effect of the soil recovery operations and the climatology on the concentration of metals and their distribution in the soil profile in an area affected by the toxic spill. Fourteen points have been selected in a plot in which acidity persists, there is no vegetation, and residual sludge stains can be seen at a glance. The temporal and spatial evolution of the extractable metals: Fe, Cu, Mn and Zn, the pH and the oxidable fraction has been measured in-depth. The results obtained up to now indicate a leaching of the pollutant towards deeper horizons, finding, at a depth of 757 cm, pH values of 3.5 and very high Fe and Mn concentrations available, especially in the profiles with large sized pores, with a big fraction of sand. On the surface, seasonally, there are low pH values of around 2.5 and extractable Fe contents of over 4000 ppm, which might have an influence on the quality of surface runoff or underground water.