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61.
Background
Congenital tremor is associated with demyelination of the brain and spinal cord and is clinically noted as outbreaks of trembling and shaking in newborn piglets during a limited time-period. Six forms of the disease have been described, where form AII may be caused by an, as yet, unidentified viral infection. This study aimed to investigate the presence of astrovirus and circovirus by sequencing and polymerase chain reaction (PCR) analysis and by relating the findings to the occurrence of disease and lesions in the brain, in 4–6 days-old piglets obtained from a clinical outbreak of congenital tremor.Results
In piglets with congenital tremor, there were mild to moderate vacuolar changes of the white matter in the cerebrum, brain stem and cerebellum. In healthy piglets, less conspicuous vacuolar changes were detected. One healthy and one diseased piglet were positive for porcine circovirus type 2. The nested pan-PCR showed the presence of astrovirus in at least one brain region in all piglets and by sequencing, two different porcine astrovirus lineages were identified.Conclusions
The results do not support previous studies identifying porcine circovirus type 2 as the cause of congenital tremor. The demonstration of astrovirus in the brain of piglets suffering from congenital tremor is interesting. However, astrovirus was demonstrated in both healthy and diseased individuals and therefore, further studies are warranted to determine the possible involvement of astrovirus in the pathogenesis of congenital tremor in pigs. 相似文献62.
Relative Contributions of Uranium, Thorium, and Potassium to Heat Production in the Earth 总被引:1,自引:0,他引:1
Data from a wide variety of igneous rock types show that the ratio of potassium to uranium is approximately 1 X 10(4). This suggests that the value of K/U approximately 1 X 10(4) is characteristic of terrestrial materials and is distinct from the value of 8 X 10(4) found in chondrites. In a model earth with K/U approximately 10(4), uranium and thorium are the dominant sources of radioactive heat at the present time. This will permit the average terrestrial concentrations of uranium and thorium to be 2 to 4.7 times higher than that observed in chondrites. The resulting models of the terrestrial heat production will be considerably different from those for chondritic heat production because of the longer half-life of U(238) and Th(238) compared with K(40). 相似文献
63.
Early Forest Soils and Their Role in Devonian Global Change 总被引:1,自引:0,他引:1
GJ Retallack 《Science (New York, N.Y.)》1997,276(5312):583-585
A paleosol in the Middle Devonian Aztec Siltstone of Victoria Land, Antarctica, is the most ancient known soil of well-drained forest ecosystems. Clay enrichment and chemical weathering of subsurface horizons in this and other Devonian forested paleosols culminate a long-term increase initiated during the Silurian. From Silurian into Devonian time, red clayey calcareous paleosols show a greater volume of roots and a concomitant decline in the density of animal burrows. These trends parallel the decline in atmospheric carbon dioxide determined from isotopic records of pedogenic carbonate in these same paleosols. The drawdown of carbon dioxide began well before the Devonian appearance of coals, large logs, and diverse terrestrial plants and animals, and it did not correlate with temporal variation in volcanic or metamorphic activity. The early Paleozoic greenhouse may have been curbed by the evolution of rhizospheres with an increased ratio of primary to secondary production and by more effective silicate weathering during Silurian time. 相似文献
64.
65.
Objective To compare serological responses in pig herds classified as low or high risk for disease caused by Actinobacillus pleuropneumoniae, using two ELISA tests based on serovar‐independent antigens. Procedure Cross‐sectional sampling was undertaken in 13 commercial herds, the clinical and slaughter histories of which indicated either freedom from (n = 5) or prior confirmed cases of A. pleuropneumoniae (n = 8). In nine herds, approximately 40 pigs each were sampled at 4, 8, 12, 16 and 20 weeks. Three of the remaining four herds were sampled between 6 and 30 weeks of age, and the last was sampled only prior to slaughter, at approximately 24 weeks. Sera were tested in ELISA based on two antigens common among A. pleuropneumoniae serovars: a 39‐kDa outer membrane protein and a recombinant ApxIVA‐N terminus protein. Results Sampling of 1 and 5 to 6‐month‐old pigs provided the most useful information on herd status. The 39‐kDa ELISA was sensitive in detecting infected herds, but had evidence of cross‐reactivity with high seroreactivity rates in older pigs in some low‐risk herds. The ApxIVA‐N ELISA was less seroreactive in high‐risk herds and had higher specificity in low‐risk herds. Conclusion ELISA based on the 39‐kDa subunit are of limited use, because of possible cross‐reactivity, but a high negative predictive value may be useful for risk assessment in suspect herds. Maternal antibody to ApxIVA‐N may be of value in detecting high‐risk herds, but 5% of 4‐week‐old pigs in low‐risk herds were also seropositive in this assay. 相似文献
66.
Desafferentation of the gastrocnemius muscle in calves with spastic paralysis suggests that exaggerated muscular tone in affected animals is due to hypersensitivity of the myotatic reflex. In order to gain more information on the pathophysiology of the disease, -efferent nerve block with dilute procaine was performed on calves with spastic paralysis, thus lowering the sensitivity of the muscle spindle.Results of this study confirm the earlier evidence of muscle spindle hypersensitivity through relative -efferent overstimulation.
Kurzfassung Die Blockierung der afferenten Reizleitung desM. gastrocremicus bei Kälbern mit spastischer Paralyse zeigt, dass ein überhöhter Muskeltonus bei erkrankten Kälbern auf Überempfindlichkeit des myotatischen Reflexes beruht. Um mehr Information über die Pathophysiologie der Erkrankung zu erhalten, wurde mit verdünntem Procain ein -efferenter Nervenblock bei Kälbern mit spastischer Paralyse induziert, um die Empfindlichkeit der Muskelspindeln zu vermindern.Die Ergebnisse dieser Untersuchungen bestätigen frühere Studien wonach die Muskelspindelüberempfindlichkeit durch relative -ableitende Überstimulierung erfolgt.
Resume Les expériences visant à supprimer les effets des fibres efferentes gamma du muscle gastrocnemien de jeunes bovins atteints de contracture des jumeaux, suggèrent que le tonus musculaire exagéré des animaux malades est dû à une hypersensibilité du réflexe myotatique. Dans le but de mieux explorer la physiopathologie de cette maladie, le blocage des fibres efferentes gamma à l'aide d'une solution de procaïne a été réalisé chez des veaux malades pour diminuer la sensibilité de la fibre musculaire.Les résultats de ce travail confirment l'existence d'une hypersensibilité primitive de celle-ci en relation avec une hyperstimulation des fibres efferentes gamma.
Riassunto La desafferentazione del muscolo gastrocnemico praticata in vitelli con paralisi spastica indica che l'eccessivo tono muscolare osservato negli animali colpiti è dovuto ad ipersensibilità del riflesso miotattico. Allo scopo di raccogliere maggiori dati sulla fisiopatologia della malattia, si è proceduto al blocco dei nervi -efferenti con procaina diluita in vitelli con paralisi spastica in modo da abbassare la sensibilità del fuso muscolare.I risultati della studio confermano quanto provato in precedenza, e cioè che l'ipersensibilità del fuso muscolare deriva dalla iperstimolazione dei relativi nervi -efferenti.相似文献
67.
Wally M. Blaney Monique S. J. Simmonds Steven V. Ley James C. Anderson Stephen C. Smith Anthony Wood 《Pest management science》1994,40(2):169-173
Decalin and dihydrofuranacetal fragments related to those in azadirachtin exhibited antifeedant activity against larvae of the African leaf-worm Spodoptera littoralis. Boisd. All the decalin fragments tested were methoxy (C11) derivatives of azadirachtin. The most active decalin fragment had a ketone substitution at C7. Overall, the compounds were more active when tested in combinations of one decalin fragment and one dihydrofuranacetal fragment than when tested singly. Although some of these combinations did show significant levels of antifeedant activity, sometimes coupled with a synergistic effect, they were not as active as either azadirachtin or dihydroazadirachtin. 相似文献
68.
William B. Ley DVM MS Diplomate ACT Craig D. Thatcher DVM MS PhD William S. Swecker DVM PhD Pierre N. Lessard DVM MPVM 《Journal of Equine Veterinary Science》1990,10(3)
Twenty-four Thoroughbred mares with breeding histories of having been barren for at least the previous 12 months were evaluated in a double-blind feeding trial to determine the effects of chelated mineral supplementation upon subsequent breeding performance. At the start of the trial (Day 0), mares were split into three dietary treatment groups of eight mares each and fed: (a) control diet with macro- and microminerals fed at NRC recommended levels for maintenance of the mature horse, (b) chelated minerals supplementation and (c) inorganic minerals supplementation at levels equal to Group B. Sixty days after initiation of the trial, there were no differences (P>0.05) between age, endometrial biopsy classification, calculated body weight and body condition score for mares between dietary treatment groups. A breeding performance trial was initiated at this time (Day 60). The number of first cycle and cumulative (i.e. over 3 estrous cycles) conceptions, number of first cycle and cumulative pregnancies and the number of total embryonic losses were not different (P>0.05) between the control and either the chelated mineral or the inorganic mineral groups. There were no harmful effects observed from feeding the chelated minerals 60 days prior to, and during, this breeding trial. 相似文献
69.
The technique of soil thin-layer chromatography has been adapted to permit the use of repeated or prolonged irrigation procedures so that the mobility characteristics of relatively non-mobile insecticides may be studied. The procedure has been used to study the relative movement of organophosphorus insecticides such as chlorfenvinphos, trithion, delnav and dichlofenthion which are widely used as sheep dips. 相似文献
70.
Evolution of mammals and their gut microbes 总被引:3,自引:0,他引:3
Ley RE Hamady M Lozupone C Turnbaugh PJ Ramey RR Bircher JS Schlegel ML Tucker TA Schrenzel MD Knight R Gordon JI 《Science (New York, N.Y.)》2008,320(5883):1647-1651
Mammals are metagenomic in that they are composed of not only their own gene complements but also those of all of their associated microbes. To understand the coevolution of the mammals and their indigenous microbial communities, we conducted a network-based analysis of bacterial 16S ribosomal RNA gene sequences from the fecal microbiota of humans and 59 other mammalian species living in two zoos and in the wild. The results indicate that host diet and phylogeny both influence bacterial diversity, which increases from carnivory to omnivory to herbivory; that bacterial communities codiversified with their hosts; and that the gut microbiota of humans living a modern life-style is typical of omnivorous primates. 相似文献