蜂王浆对果蝇寿命及抗氧化酶活力的影响

用涂布了不同剂量蜂王浆(0.1、0.3、0.5 g)的培养基饲养果蝇,测定蜂王浆对果蝇寿命和两种抗氧化酶活性的影响.研究结果表明,蜂王浆能延长果蝇的平均寿命,且呈剂量效应,其中高剂量组雄、雌果蝇的平均寿命比对照组长24.7%和26.9%.研究没有发现蜂王浆对果蝇的最高寿命有明显影响.对果蝇超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力测定结果表明,蜂王浆能够提高果蝇的SOD和CAT活性,说明蜂王浆具有增强清除自由基的能力,从而具有一定的延缓衰老的功能.     

生态环保型效益经济的特征与内涵

在新世纪,生态经济已成为国际经济发展的主流,成为中国各省、市、区经济建设的发展方向。用绿色意识重新审视传统的经济发展模式,从根本上转变资源依赖型的产业结构和粗放型的经济增长方式,是新一轮经济发展的客观要求。吉林省提出生态环保型效益经济的全新发展模式,以发展生态环保型效益经济为中心进行生态省建设,走一条具有鲜明区域经济特色的可持续发展道路,是历史发展的必然选择     

塔里木河下游输水与生态修复

塔里木河是我国最长的内陆河。为了抢救塔里木河下游生态环境,2000~2003年向断流30余年的下游500 km左右的天然河道,实施了5次应急输水。在收集了对5次输水后生态修复评价的大量资料的基础上,结合第6次和第7次输水,对生态响应进行了进一步研究,并提出了进一步扩大输水生态修复影响范围的人工辅助措施,通过分河段、分区域合理配置水资源,以实现塔河流域生态修复的总体目标。     

Isolation,Culture and Identification of Buffalo Mammary Epithelial Cells from Milk

This study was aimed to set up a simple, economic and pure method to culture and identify buffalo mammary epithelial cell in vitro, which were collected from the fresh milk. The milk was collected in the late lactation period of the high yield milk buffalo, then it was mixed with the PBS in the ratio of 2:1 and centrifugated. A few suspernatants and the pellets were resuspended with the PBS when the milk fat were decanted, then recntrifuged. The final pellets and 1 mL of suspernatants were seeded in petri dish without fat, respectively. The isolated cells were mammary epithelial cells which were identified by cell morphology, immunofluorescence, growth curve, cell viability and RT-PCR. The buffalo mammary epithelial cells were successfully isolated from both the pellets and supernatants. The cells and impurities were less in the supernatants than that in the pellets. The cells were cobblestone-like without fibroblasts and most in cell division. In the post-confluent culture, mammary epithelial cells formed dome structures and layer-separated growth. The cells expressed cytokeratin 18 was identified by immunofluorescence which was the marker of mammary cells. The cell growth curve and cell survival rate were measured. The results showed that the buffalo mammary epithelial cells were activity and easy to attach. The mammary epithelial cells were expressed of milk protein and milk fat related genes detected by RT-PCR. The buffalo mammary epithelial cell line were successfully isolated and identified from fresh milk, which estabilished foundation for the study of the mechanism of lactation, the amelioration of the quality of milk and the improvement of milk yield of buffalo.     

野生木耳担孢子萌发特性研究

采用振荡培养法研究了野生木耳担孢子的萌发特性。结果表明,木耳担孢子在无菌水中培养24 h不能萌发,36h时可萌发产生芽管,60 h后芽管出现分支,各分支顶端产生1个~4个分生孢子。试验发现,木耳担孢子可直接萌发产生初生菌丝或分生孢子。     

高温胁迫对几种乔木树种生理生化特性的影响

利用人工气候箱控制温度,在相对湿度40％的条件下,分别在30℃、35℃、39℃、41℃的高温胁迫下,对盆栽臭椿、白蜡、光皮树、黄连木、构树5个树种离体叶片的生理生化特性进行研究.结果表明:在试验温度范围内,臭椿、黄连木和构树的相对电导率最大值出现在35℃时,分别为50.43％、94.30％和81.26％;光皮树和白蜡的...     

几种兽用合成抗菌药基于抗体快速检测技术研究进展

合成抗菌药在预防、治疗动物疾病等方面应用十分广泛,但不合理使用造成的耐药及残留问题日益严重,因此对兽用合成抗菌药的残留检测势在必行,而基于抗体的快速检测技术具有简便、灵敏度高、成本低等优点,发展前景十分广阔。作者综述了近年来针对磺胺类、氟喹诺酮类、喹噁啉类、硝基呋喃类、硝基咪唑类5大类兽用合成抗菌药物的抗体检测技术最新研究趋势,旨在促进残留快速检测技术的发展。     

碱性蛋白酶提取米糠蛋白的研究

为建立高效的米糠蛋白提取工艺,对碱性蛋白酶提取米糠蛋白的工艺条件进行了研究。以蛋白提取率为考察指标,在单因素试验的基础上,通过正交试验进一步优化米糠蛋白提取工艺条件。结果表明,料液比对米糠蛋白提取率的影响最大,其次为pH值,温度、时间和加酶量影响较小。优化后的工艺条件为:温度60℃,时间3.0h,料液比1∶20,加酶量2.5%,pH值9.5。在此条件下,蛋白提取率可达75.42%,同时测得米糠蛋白的等电点为4.5。影响米糠蛋白提取率的主次因素顺序为:料液比>pH值>温度>时间>加酶量。     

Advances in the Corn Fibers

Corn fiber is a concept proposed in 1948,which involves in the polymeric fiber that made from corn as the raw material, and it was turned to lactic acid, transformed into lactide, and polymerized to polylactide or polylactic acid. Since that, some progresses have been made. However, the feature of corn fiber is determined by the molecular weight of polylactide. In general, corn fiber is formed by means of the melt-textile or no-hydrous textile. The situations, technologies, properties of the corn fibers are reviewed, as well as the processing about lactide synthesis, lactide condensation polymerization. It has some professional properties, such as high transparency, well-heat-resistant stability, well-colors, and full biodegradability, as well as the feature in ecological recycle. These means the corn fibers are new-typical and green, full-environmental fibers, especially for China as the second great number of the products of corn. Research and development of corn fibers are launched off in the world. Some problems existes in the corn fiber manufactured field are to be evolved, which are small scale, high cast, little of end-products enrolled in the market. So is down the cast of corn fiber manufactured as the very important works. This means that systemic studies of the fiberous polymerization and processing are crucial and highlighted to in this field.     

泡沫镍负载 Cu/CuO 微纳结构的制备及其对抗坏血酸的检测研究

利用水热法制备了高电活性的泡沫镍负载Cu/CuO微纳结构,形貌与成分分析表明,该电极材料从内到外共包含泡沫镍基体、Cu微米颗粒层和CuO纳米结构层三层结构.电化学测试发现该复合材料在碱性介质中对抗坏血酸具有很好的电催化氧化特性,其灵敏度达到6.14mA/(mmol/L·cm2),探测范围为1~2.5mmol/L.