THE SIMULATION OF ANIMAL RETURNS IN GRASSLAND EXPERIMENTATION

An experiment was carried out on a grass/white-clover sward at North Wyke to evaluate the use of mixtures of inorganic and organic nutrient sources to simulate the nutrient effect of animal excreta, both with and without overall PK fertilizer. Grazing with sheep and the return of excreta from caged sheep both increased herbage yields, as did artificial return treatments. Artificial return treatments giving 75% of the nutrient level returned by excreta gave yields comparable with grazing. The effect of the simulated return treatments was not influenced by PK application. Grazing gave a very different botanical composition to that from the treatments in wbich natural or simulated excreta were returned to cut swards. Mixtures simulating excreta may replace the nutrient effect of the grazing animal, but not its physical effect, in some forms of grassland experimentation. They are not recommended for fertilizer trials.     

Survival of soil bacteria during prolonged desiccation

A determination was made of the kinds and numbers of bacteria surviving when two soils were maintained in the laboratory under dry conditions for more than half a year. Certain non-spore-forming bacteria were found to survive in the dry condition for long periods. A higher percentage of drought-tolerant than drought-sensitive bacteria was able to grow at low water activities. When they were grown in media with high salt concentrations, bacteria generally became more tolerant of prolonged drought and they persisted longer. The percent of cells in a bacterial population that remained viable when exposed to drought stress varied with the stage of growth.     

THE RATE OF WATER LOSS FROM GRASS SAMPLES DURING HAY-TYPE CONSERVATION

The loss of water from small bulk samples of five grass species was studied under constant conditions of humidity, temperature and lighting. A logarithmic curve was fitted to the data and the different species compared on the basis of a loss coefficient derived from the fitted curve. The species varied significantly in this aspect; those with high surface-area to dry-weight ratios generally lost water fastest The presence of stem material appeared to increase the rate of water loss considerably. High humidity during drying caused the grasses to lose water more slowly and to retain much more water at equilibrium with the surrounding air. Chemical treatments to speed water loss, using a solvent and a fungal toxin were tested. Both appear to have some promise. Selection for high water loss in grass breeding may also be possible.