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201.
Nicholas Bacon Carlos H. de M. Souza Sarah Franz 《The Canadian veterinary journal. La revue veterinaire canadienne》2016,57(2):141-146
We describe a novel technique for total cysto-prostatectomy, followed by uretero-urethral anastomosis in 2 dogs. The technique was successful and was performed without pubic osteotomy. Post-operative urinary tract infections may be a potentially serious event. 相似文献
202.
B. G. Welderufael D. J. de Koning L. L. G. Janss J. Franzén W. F. Fikse 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2016,66(3):125-134
The aim of this study was to develop a new approach for joint genetic evaluation of mastitis and recovery. Two mastitis incidences (0.28 and 0.95) measured via somatic cell count and three between traits genetic correlations (0.0, 0.2, and ?0.2) were simulated for daughter group sizes of 60 and 240. A transition model was applied to model transitions between healthy and disease state. The RJMC package in DMU was used to estimate (co)variances. Heritabilities were consistent with the simulated value (0.039) for susceptibility and a bit upward biased for recovery. Estimates of genetic correlations were ?0.055, 0.205, and ?0.192 for the simulated values of 0.0, 0.2, and ?0.2, respectively. For daughter group size of 60, accuracies of sire EBV ranged from 0.56 to 0.69 for mastitis and from 0.26 to 0.48 for recovery. The study demonstrated that both traits can be modeled jointly and simulated correlations could be correctly reproduced. 相似文献
203.
Successful Cryopreservation of Domestic Cat (Felis catus) Epididymal Sperm after Slow Equilibration to 15 or 10°C 下载免费PDF全文
To support conservation strategies in wild species, simple but highly reproducible procedures of sperm cryopreservation are required for an application under field conditions. We used epididymal sperm of the domestic cat to optimize a sperm freezing procedure for felid species, particularly questioning the demand for sperm cooling to 4°C. We equilibrated sperm during slow cooling to only 15 or 10°C in a Tes–Tris–fructose extender with final concentrations of 4.7% (v/v) glycerol and 10% (v/v) of the water‐soluble fraction of hen's egg yolk (low‐density lipoproteins). Subsequently, sperm were frozen over liquid nitrogen. Total and progressive motility (mean ± SD) after thawing was 60.7 ± 8.6% and 53.9 ± 9.6% in samples cooled to 15°C or 61.6 ± 9.5% and 55.3 ± 9.9% in samples cooled to 10°C. Therefore, a one‐step addition of glycerol to sperm at room temperature together with the freezing extender, the use of cryovials (loaded with diluted sperm aliquots of 300 μl), an equilibration period of 40 min comprising slow cooling to 15°C at a rate of approximately ?0.14 K/min before rapid freezing over liquid nitrogen, yielded satisfying results. Cooling, freezing and thawing rates were exactly characterized as a prerequisite for further optimization and to provide a repeatable protocol to other practitioners. 相似文献
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Franz Berthiller 《中国家禽》2014,(2)
检测食品中霉菌毒素的一般流程为采样、研磨、提取、过滤、净化、检测和评估。本文对这一检测流程的具体步骤进行分析,认为采样是霉菌毒素检测误差的主要来源,简便方法主要基于免疫学检测系统,LC-MS/MS法越来越多地应用于多毒素的同时检测,而稳定同位素稀释法用于食品和饲料中霉菌毒素的准确定量检测。 相似文献
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