蓖麻毒蛋白A链基因克隆与重复表达载体构建

利用降落PCR的方法,从蓖麻基因组DNA中克隆毒蛋白A链基因的560bp片段;利用一步法将该基因的2个正义片段和2个反义片段分别与pBI-121质粒连接,构建含该基因的正义重复和反义重复表达载体,为利用共抑制技术抑制蓖麻毒蛋白A链基因表达的研究奠定基础。     

Salmonella in captive and wild Tasmanian devils (Sarcophilus harrisii) in Tasmania

Translocation of Tasmanian devils (Sarcophilus harrisii) is a common strategy for recovery of the species as carried out by the Save the Tasmanian Devil Program. Dasyurids including the endangered Tasmanian devil are well known to asymptomatically harbour the zoonotic bacteria Salmonella enterica in their intestinal tracts. Testing for Salmonella is a routine component of pretranslocation health testing, so a statewide microbiological survey of captive and wild devils was implemented in order to understand prevalence and common Salmonella serotypes, and inform decision-making when positive cultures are identified. This preliminary study identified a significantly higher proportion of Salmonella isolations in wild compared with captive devils. Mississippi and Typhimurium were the most common serotypes, followed by Lexington, Bovismorbificans, Kottbus and Amsterdam. Given the common finding of Salmonella in wild devils and the range of serotypes involved, in addition to numerous isolations in domestic species and humans, it is unlikely that the release of small numbers of captive devils to the wild in Tasmania poses a significant risk to the destination ecosystem. Ongoing monitoring of devils is required as the stress of acclimatisation could predispose devils to clinical disease. Appropriate personal protective attire is pertinent to protect personnel handling animals from this zoonotic infection.     

Pulmonary mycobacteriosis caused by Mycobacterium intracellulare in a Tasmanian devil (Sarcophilus harrisii)

The health of captive Tasmanian devils (Sarcophilus harrisii) is currently of increased interest because wild populations are being decimated by the spread of devil facial tumour disease. This report describes the pathology of an aged captive Tasmanian devil that had a pulmonary mycobacterial infection caused by Mycobacterium intracellulare in addition to multiple neoplastic processes.     

宁夏荒漠草原蒙古冰草资源分配特征

为了解不同群落生境蒙古冰草(Agropyron mongolicum)资源权衡策略及种群维持机制,在宁夏荒漠草原选取以蒙古冰草为优势种或亚优势种的群落为研究对象,研究了不同群落蒙古冰草资源分配特征。结果表明：(1)蒙古冰草个体生物量及根冠比在不同群落之间差异显著(P<0.05),构件生物量分配均表现为根>茎>叶>穗;(2)不同群落蒙古冰草各构件C,N,P含量均以根部最低,C/N,C/P以茎部最高,N/P以叶部为高,比值范围为18～23;不同群落之间则表现为C/N和C/P总体以M2群落较高,地上部各构件N/P以M3群落较高(P<0.05);(3)蒙古冰草地下生物量与土壤含水量呈显著负相关,与各构件N,P元素含量呈显著正相关;各构件P元素含量与其生物量分配比及地上地下生物量之间存在显著相关关系(P<0.05)。综上,蒙古冰草可通过调整自身构件分配格局和养分利用策略保证其在不同群落生境的生长发育和繁殖。