切花菊病毒脱毒及脱毒苗的检测

 菊花切花的生产量在整个切花生产中占比例最大,高达30%,它是世界上栽培最普遍、销售量最大的花卉之一。能侵染菊花的病毒多达近二十种。     

Involvement of PAPP‐A and IGFR1 in Cystic Ovarian Disease in Cattle

Cystic ovarian disease (COD) is one of the main causes of infertility in dairy cattle. It has been shown that intra‐ovarian factors, such as members of the insulin‐like growth factor (IGF) system, may contribute to follicular persistence. The bioavailability of IGF to initiate its response by binding to specific receptors (IGFRs) depends on interactions with related compounds, such as pregnancy‐associated plasma protein A (PAPP‐A). The aim of this study was to determine IGFR1 and PAPP‐A expression both in follicles at different stages of development and in cysts, to evaluate the roles in the etiopathogenesis of COD in cattle. The mRNA expression of PAPP‐A was higher in granulosa cells of large tertiary follicles than in cysts, whereas the protein PAPP‐A present in the follicular fluid from these follicles showed no differences. Although no PAPP‐A mRNA expression was detected in smaller tertiary follicles, in their follicular fluid, this protease was detected in lesser concentration than in cysts. The mRNA expression of IGFR1 was lower in granulosa cells from cystic follicles than in those from tertiary ones. However, the protein expression of this receptor presented the highest levels in cystic structures, probably to increase the possibility of IGF response. The data obtained would indicate that animals with COD have an altered regulation of the IGF system in the ovary, which could be involved in the pathogenesis of this disease in cattle.     

Growing potato plants by the water culture technique

A water culture technique was developed for the study of the nutrient requirements of the potato plant. Tuber formation was avoided by pinching off the stolons as they developed and hence vegetative growth could be studied without the complication of abnormal tuber formation in the culture solution. The K deficiency symptoms obtained by this method were essentially the same as those observed for field grown plants.     

Estrus Synchronization in Sheep and Goats Using Combinations of GnRH,Progestagen and Prostaglandin F2α

The objective of this experiment was to evaluate the effect of GnRH, progestagen and prostaglandin F_2α on estrus synchronization in sheep and goats. Sixty Awassi ewes and 53 Damascus does were used in the study. The experiment started at the beginning of the breeding season (June/July). The same treatments were applied to sheep and goats as follows: no treatment (CON), 14‐day progestagen sponges and 600 IU equine chorionic gonadotropin (S), gonadotropin releasing hormone followed 5 days later by prostaglandin F_2α (GP) and gonadotropin releasing hormone, progestagen sponges for 5 days and prostaglandin F_2α on the day of sponge removal (GSP). None of the ewes in the S group lambed from mating during the induced cycle. A greater lambing rate (p < 0.05) was observed in the GSP group compared with the CON and S groups while the GP group was intermediate. The number of lambs born per lambed ewe was similar among the CON, GP and GSP groups. However, the number of lambs per exposed ewe was greater (p < 0.05) in the GSP than the remaining groups. The induced cycle kidding rate was 77% for all treatments combined. Similar kidding rate were observed among treatments. The numbers of kids born per kidded and exposed doe from mating during the induced estrus were also similar among treatments. Greater numbers of multiple births were observed in the GP and GSP than in the S group. In conclusion, a combination of GnRH, progestagen sponges and PGF_2α can be effective in synchronizing estrus and improving fecundity in sheep and goats. Although the use of GnRH–PGF_2α was effective, the addition of progestagen sponges at the time of GnRH administration appeared to improve reproductive parameters.     

Model studies on the oxygen-induced formation of benzaldehyde from phenylacetaldehyde using pyrolysis GC-MS and FTIR

Benzaldehyde, a potent aroma chemical of bitter almond, can also be formed thermally from phenylalanine and may contribute to the formation of off-aroma. To identify the precursors involved in its generation during Maillard reaction, various model systems containing phenylalanine, phenylpyruvic acid, phenethylamine, or phenylacetaldehyde were studied in the presence and absence of moisture using oxidative and nonoxidative Py-GC-MS. Analysis of the data indicated that phenylacetaldehyde, the Strecker aldehyde of phenylalanine, is the most effective precursor and that both air and water significantly enhanced the rate of benzaldehyde formation from phenylacetaldehyde. Phenylpyruvic acid was the most efficient precursor under nonoxidative conditions. Phenethylamine, on the other hand, needed the presence of a carbonyl compound to generate benzaldehyde only under oxidative conditions. On the basis of the results obtained, a free radical initiated oxidative cleavage of the carbon-carbon double bond of the enolized phenylacetaldehyde was proposed as a possible major mechanism for benzaldehyde formation, and supporting evidence was provided through monitoring of the evolution of the benzaldehyde band from heated phenylacetaldehyde in the presence and absence of 1,1'-azobis(cyclohexanecarbonitrile) on the ATR crystal of an FTIR spectrophotometer. In the presence of the free radical initiator, the enol band of the phenylacetaldehyde centered at 1684 cm(-1) formed and increased over time, and after 18 min of heating time the benzaldehyde band centered at 1697 cm(-1) formed and increased at the expense of the enol band of phenylacetaldehyde, indicating a precursor product relationship.     

解冻期覆盖盐渍土地表对土壤盐分和水分的影响

选择春季土壤解冻期,用塑料薄膜、麦草和沙子覆盖盐渍土地面,改良盐碱地。结果表明,覆盖对土壤盐分表聚和水分蒸发损失具有明显的抑制作用。塑料薄膜采用全地面覆盖,麦草适用量为2934．8k／hm^2沙子的适宜覆盖厚度为4cm。     

High pressure liquid chromatographic determination of methomyl and oxamyl on vegetable crops

A reverse phase high pressure liquid chromatographic method is presented for the separation and determination of residues of the carbamates oxamyl and methomyl on vegetables. A liquid-liquid extraction and cleanup procedure is applied to the vegetable extract. Samples are eluted from a muBondapak C18 column and quantitated by ultraviolet absorbance at 240 nm. Recovery data for vegetable samples spiked at 2 ppm are presented.     

Locally Grown Oysters in Hawai‘i: Chef Preference and Local Premium?

Locally grown oysters became available in Hawai‘i in 2014, for the first time in decades. It is unclear if these bivalve shellfish will successfully infiltrate the market when imported products similar in nature have long been the only source of fresh, shell stock products. This study investigates chef preferences for oyster attributes to determine marginal willingness to pay for locally grown oysters. A survey containing a questionnaire and a choice experiment was administered to restaurant chefs throughout Hawai‘i who currently offer oysters on their menus. The study examines trade‐offs made between attributes related to price, growout site, freshness, and supply constancy, respectively. Responses were aggregated and data were fit to a random parameters logit model. Results suggest that respondents derive the most utility from oysters that are fresh, grown locally, and supplied on an as‐available basis. The least desired products are, therefore, those that are frozen, imported from foreign sources, and supplied on a set schedule. Chefs are willing to pay an additional $5.25 for one dozen oysters that are grown locally. This evidence of an existing price premium indicates that labeling local oysters as such may be a valuable marketing strategy.