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41.
We assessed the effects of nutrient supply and dietary bulk, both increasing with hay intake, on O2 uptake and nutrient net fluxes across the portal-(PDV) and mesenteric- (MDV) drained viscera, and the rumen in adult ewes. Four ewes, fitted with a ruminal cannula, with catheters in the mesenteric artery, the portal, mesenteric and right ruminal veins, and with a blood flow probe around the right ruminal artery, were used in a 4 x 4 Latin square design. Treatments consisted of 500 g DM/d hay (LL, low bulk and low nutrient supply), 500 g DM/d hay + infused nutrients (LH, low bulk and high nutrient supply), 750 g DM/d hay + infused nutrients (MH, medium bulk and high nutrient supply), and 1,000 g DM/d hay (HH, high bulk and high nutrient supply). Infused nutrients consisted of volatile fatty acids (VFA) and casein dissolved in salts and infused continuously in the rumen to provide the same amount of metabolizable energy (7.6 MJ/d) and digestible protein (63 g/d) for LH, MH, and HH. Both increases in bulk and nutrient supply increased O2 uptake in the MDV and PDV. Dietary bulk stimulated mainly blood flow, whereas nutrient supply stimulated mainly O2 extraction rate. The O2 uptake by the rumen was not significantly affected by hay intake, although blood flow increased due to nutrient supply. Increase in hay intake had no effects on portal net release of lactate and net uptake of glucose but increased VFA, 3-D-hydroxybutyrate, ammonia, and amino acids (AA) net release and urea net uptake across PDV. The increase in portal nutrient net fluxes with hay intake was entirely related to the increase of nutrient supply for VFA, 3-D-hydroxybutyrate, ammonia, and urea, irrespective of the amount of casein infused for AA. Dietary bulk had no effect on total energy net release in the portal vein. We conclude that despite the increase in portal O2 uptake, increasing dietary bulk had no significant impact on portal recovery of energy. In ruminal tissues, which were the main site of energy absorption, O2 uptake appeared low and was not sensitive to dietary manipulation. In contrast, in mesenteric tissues, which contribute poorly to energy absorption with forage diets, O2 uptake appeared high and very sensitive to dietary manipulation. 相似文献
42.
Alley MR Connolly JH Fenwick SG Mackereth GF Leyland MJ Rogers LE Haycock M Nicol C Reed CE 《New Zealand veterinary journal》2002,50(5):170-176
AIM: This study reports an outbreak of salmonellosis due to S. Typhimurium DT160 which caused extensive mortality in wild birds and enteric disease in humans in New Zealand during the winter and spring months of the year 2000. METHODS: Necropsies were performed and microbiological examinations undertaken on wild birds from populations in which mass mortality was reported, and on captive indigenous birds which died suddenly during the winter and spring of 2000. Affected tissues were examined histologically and isolates of S. Typhimurium were phage typed and examined using pulsedfield gel electrophoresis (PFGE). Isolates of S. Typhimurium obtained from cases of human enteric disease which occurred during these months were phage typed, examined using PFGE and compared with the bird isolates. RESULTS: Central and northern areas of the South Island and the southern North Island were worst affected with die-offs of several hundreds of sparrows and other birds reported in rural areas. Mortalities reached a peak in winter (July-August) 2000 and decreased to small numbers during the spring and early summer. The birds usually died of an acute septicaemia with multifocal necrotising lesions in the liver and spleen. Human cases throughout the country increased gradually over the same period. Isolates from birds, livestock and humans examined using PFGE were indistinguishable from one another. CONCLUSION: This strain of Salmonellahas emerged as a major cause of septicaemia in wild birds in New Zealand. Because of the close association between house sparrows (Passer domesticus) and humans, the organism also poses a serious zoonotic risk. The possibility that the infection may spread to involve indigenous species needs investigation. 相似文献
43.
Adaptations of glucose metabolism in multiparous sows: effects of pregnancy and feeding level 总被引:3,自引:0,他引:3
This experiment was undertaken to determine whether pregnancy affects glucose metabolism and insulin sensitivity in sows fed at different levels. Four replicates of six multiparous Large White sows were involved. In each replicate, four sows were inseminated on the first postweaning estrus (pregnant group) and the two remaining were kept nonpregnant. Half of the sows of each group were fed 2.5 kg/d (low level) and the others 4 kg/d (high level) of the same standard pregnancy diet. Jugular catheters were implanted 2 to 3 d after estrus. Plasma concentrations of glucose, insulin, and FFA were determined before and during the 4 h following the morning meal at 10, 30, 59, 87, 93, 101, and 110 d of gestation and at equivalent periods for the nonpregnant sows. Intravenous glucose tolerance tests were performed at 33, 71, 85, 96, and 108 d by i.v. injection of 0.5 g glucose/kg BW. Compared with the glycemia before the meal, all the sows showed hyperglycemia 30 min after the initiation of the meal and hypoglycemia thereafter, with a minimum reached at approximately 75 min. Insulinemia increased from 20 min after food access, reached a maximum at 40 min, and returned to the basal level after 180 min. The higher feeding level increased plasma insulin and lowered plasma glucose levels. Glycemia and insulinemia profiles changed from 87 d onward in the pregnant sows. The peak of glucose induced by the meal was higher, and the subsequent period of hypoglycemia almost disappeared. The area under the insulin curve was unchanged, but insulin secretion was delayed. The glucose tolerance tests showed that between d 85 and 108 the half-life of injected glucose increased and insulin secretion was delayed in the pregnant sows. Compared to the following stages, plasma FFA were high before and after the meal at 10 d, which most likely was a residual effect from the previous gestation/lactation cycle. They were lower from 30 to 101 d in the pregnant and nonpregnant sows. At 110 d, fasting FFA were high again in the pregnant sows only, very likely in relation to the preparation for lactation. This experiment showed that insulin sensitivity decreases after 85 d of pregnancy in multiparous sows. 相似文献
44.
Albina E Mesplède A Chenut G Le Potier MF Bourbao G Le Gal S Leforban Y 《Veterinary microbiology》2000,77(1-2):43-57
In early 1992, a CSF epizootic was clinically recognised in a wild boar population of approximately 1300 animals within an area of 250km(2) located in the east of France. In order to check the CSF situation in wild boars outside this area, a serological survey was carried out in the rest of France, for 8 consecutive years (1991-1998). This paper reports on the results obtained during this survey which included wild boars shot during the hunting period but also boars reared within fences. Around 1000-2700 sera a year were tested for the presence of antibodies to classical swine fever virus (CSFV) and also to Aujeszky's disease virus (ADV). Out of 12025 sera tested over the whole period, 80 wild boars were found positive for CSF antibodies. Sixty of them were collected on wild boars shot during the years 1992-1994 in the epizootic area located in east of France and 10 were collected in Corsica during the years 1994-1996. The last four positive samples were single reactors coming from areas or farms, which were thereafter confirmed to be serologically negative. These results together with the fact that no disease has been reported so far illustrate that the French wild boar population is probably not concerned by CSF infection (excepted in the east of France where the disease has now become enzootic). Two hundred and forty nine sera were initially detected as CSF positive but confirmed secondarily as positive for border disease (BD) antibodies. This finding shows that wild boars are also susceptible to infection by ruminant pestiviruses. Four hundred and twenty three wild boars have been found positive for ADV antibodies. In addition, from 1993 to 1995, 909 samples were tested for the presence of antibodies to porcine reproductive and respiratory syndrome virus (PRRSV). Thirty three of them were positive. The results on AD and PRRS antibody detection show that wild boars may constitute a reservoir for various infectious diseases of pigs. 相似文献
45.
S. Giosuè G. Spada V. Rossi G. Carli I. Ponti 《European journal of plant pathology / European Foundation for Plant Pathology》2000,106(6):563-571
An Israeli model forecasting leaf curl disease on peaches caused by Taphrina deformans was validated in the Emilia-Romagna region of northern Italy, during a three-year period (1996–1998), in 13 cases (year × location × cultivar). When the peach trees are susceptible to infection, the model uses mathematical functions to calculate the risk of infection on the basis of weather conditions (daily rainfall greater than 10mm, and maximum air temperature greater than 5°C), and it forecasts periods of possible symptom appearance based on the length of incubation. Peach trees became susceptible to infection between the end of January and mid March, when the first leaf buds attained phenological stage C, i.e. appearance of leaf apex. The trees remained susceptible for at least 9 weeks: the last infection occurred in mid-May.Since most of the leaf curl onsets observed in the orchards fell within the range of model forecasts, the model proved to be accurate in signalling both the first seasonal infection and repeated infections during the primary inoculum season. Few errors occurred, caused either by conditions of rainfall and temperature lower than the thresholds fixed in the model, or by discrepancies between forecast and actual length of incubation. Infection occurred also at 3.1–3.5°C, and with 9.6mm rainfall. Thus, thresholds should not be accepted too rigorously, and perhaps temperature should not be considered as a limiting factor for infection under the conditions of the present work. The length of incubation showed high variability: it was 23 days long on average, with a 95% confidence interval ranging from 20 to 27 days, and extreme values of 9 and 33 days. 相似文献
46.
Albertine Léon Stéphane Pronost Jackie Tapprest Nathalie Foucher Béatrice Blanchard Geneviève André-Fontaine Claire Laugier Guillaume Fortier Roland Leclercq 《Journal of veterinary diagnostic investigation》2006,18(2):218-221
Studies were carried out to determine the cause of death in a prematurely born Thoroughbred foal that died 24 hours after birth. Necropsy revealed gross lesions suggestive of septicemia. A commercial Leptospira polymerase chain reaction (PCR) assay designed to specifically amplify the hemolysis-associated protein 1 (hap1) gene present only in pathogenic Leptospira strains detected the presence of Leptospira DNA in various tissues of the foal. Histologic examination of lung, liver, kidney, and myocardium revealed numerous spirochetes in Warthin-Starry-stained tissue sections. Results of PCR analysis and histologic examination suggested a leptospiral infection in the newborn foal. At the moment of death, the infection coexisted with a streptococcal-associated aspiration bronchopneumonia and postpartum septicemia. These findings indicate that the PCR assay based on the amplification of the hap1 gene represents a useful tool for specific detection of pathogenic leptospira in field samples taken from horses. 相似文献
47.
Susan J. Sprague Marie-Hélène Balesdent Hortense Brun Helen L. Hayden Stephen J. Marcroft Xavier Pinochet Thierry Rouxel Barbara J. Howlett 《European journal of plant pathology / European Foundation for Plant Pathology》2006,114(1):33-40
Resistance of Brassica napus (oilseed rape, canola) conferred by three different major resistance genes has been overcome by changes in virulence of Leptosphaeria maculans populations in France and Australia. In South Australia where B. napus cultivars with major gene resistance derived from Brassica rapa ssp. sylvestris were grown extensively, resistance was rendered ineffective within 3 years of commercial release of the cultivar. Disease
severity was higher on cultivars with sylvestris-derived resistance than cultivars with polygenic resistance. This Australian situation is compared to that in France, where
resistance conferred by the Rlm1 gene was overcome nation-wide in 5 years under commercial cropping practices, and also where a source of resistance introgressed
into B. napus from B. juncea was rendered inefficient in 3 years in experimental field plots near Rennes. 相似文献
48.
Randomized control trial to test the effect of a feed additive on Campylobacter contamination in commercial broiler flocks up to slaughter
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A. Huneau‐Salaün M. Guyard‐Nicodème G. Benzoni X. Gautier S. Quesne T. Poëzevara M. Chemaly 《Zoonoses and public health》2018,65(4):404-411
A randomized controlled trial (RCT) was carried to evaluate the effect of a feed additive on Campylobacter contamination of broilers reared in commercial conditions. Twenty‐four broiler flocks naturally contaminated with Campylobacter were enrolled in the RCT: 12 were assigned to a control group (C) fed with a conventional finishing feed from 4 weeks of age to slaughter (around 35 days), and the other group of 12 flocks (S) was fed with a finishing feed supplemented with 250 ppm of a patented feed additive (an ion‐exchanged clay compound) previously proven to reduce Campylobacter contamination in broiler caeca under experimental conditions. Enumeration of Campylobacter colonies in caeca (8 per flock) was carried out following ISO standards before feed distribution and at slaughter. Before treatment, the caecal Campylobacter load tended to be lower in C flocks (7.1 ± 1.9 log CFU/g, CI95% [6.6–7.5]) than in S flocks (7.7 ± 1.0 log UFC/g, CI95% [7.5–7.9]) (p = .05). At slaughter, the bacterial load was similar in the S (7.7 ± 1.0 log CFU/g, CI95% [7.5–7.9]) and C groups (7.5 ± 1.2 log CFU/g, CI95% [7.2–7.8]) (p = .73). Therefore, the feed additive had no significant effect on the caecal Campylobacter load at slaughter under the tested conditions. The logistical constraints inherent in field trials and the natural variability of Campylobacter contamination in naturally infected broiler flocks make it difficult to reproduce experimental results in in situ farm conditions. RCT testing of an intervention strategy in commercial situation is therefore a key step in evaluating pre‐harvest interventions against food‐borne pathogens. 相似文献
49.
Thibaut Olivier Vaidevutis Šveikauskas Elisabeth Demonty Kris De Jonghe Pascal Gentit Mojca Viršček-Marn Sabine Grausgruber-Gröger Sébastien Morio Francesco Faggioli Michèle Visage Frédéric Fauche Maria Gusina Marta Luigi Helena Lasner Irena Mavrič-Pleško 《European journal of plant pathology / European Foundation for Plant Pathology》2016,144(3):645-654
50.
Effects of harvesting perennial ryegrass at different levels of herbage mass on voluntary intake and in vivo digestibility in sheep
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M. Beecher R. Baumont M. O'Donovan T. M. Boland J. Aufrère C. Fleming N. Galvin E. Lewis 《Grass and Forage Science》2018,73(2):553-561
The production performance of herbage‐fed animals is affected by herbage voluntary dry‐matter intake (VDMI) and organic matter digestibility. The objective of this study was to determine the effect of herbage mass (HM) on in vivo herbage voluntary intake and digestibility in sheep. The three HM treatments were as follows: 1,100 kg dry matter (DM)/ha (low, L), 2,300 kg DM/ha (medium, M) and 3,700 kg DM/ha (high, H). The study was a Latin square design, repeated on two occasions in 2012: 24 May to 20 July (summer) and 21 July to 5 October (autumn). Twelve Texel wether sheep (individually housed) were offered fresh cut perennial ryegrass herbage for ad libitum consumption. Using the total faecal collection method, the digestibility of dry matter, organic matter, neutral detergent fibre and acid detergent fibre was determined. In summer, L had a similar VDMI to M and both had a higher VDMI than H. In autumn, L had a higher VDMI than both M and H. For dry‐matter digestibility and organic matter digestibility, there was no significant difference between L and M, which were both higher than H. For every 1% increase in neutral detergent fibre digestibility, VDMI increased by 0.03 kg. In the zero‐grazing scenario examined, offering low (1,100 kg DM/ha) HM swards enabled animals to achieve high intakes of highly digestible herbage, which should ensure high animal production performance. 相似文献