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101.
Sobia Ajaz Harriet R. Benbow Thalia Christodoulou Cristobal Uauy Fiona M. Doohan 《Plant pathology》2021,70(5):1123-1137
Globally, bread wheat production is threatened by fungal diseases, including the devastating disease Septoria tritici blotch (STB). Given the global importance of STB, and the difficulty in identifying novel sources of resistance to this disease, we screened a variety of wheat genotypes, including wild, ancestral, and mutagenized lines, for their STB response. This delineated a panel of wild wheat relatives and Watkins collection lines with exceptional resistance to a range of Zymoseptoria tritici isolates, some of which are highly virulent on modern, elite wheat varieties. Additionally, we characterized the STB susceptibility of 500 lines of the wheat cultivar Cadenza TILLING population and developed backcross derivatives of two TILLING lines that show dominant partial resistance to STB. These backcross lines are partially resistant to multiple isolates of Z. tritici, and, with the wild and ancestral lines identified, provide a useful reservoir of STB-resistant germplasm for use in wheat breeding programmes. 相似文献
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Tracy A. Clegg Teresa Morrissey Fiona Geoghegan S. Wayne Martin Kieran Lyons Seán Ashe Simon J. More 《Preventive veterinary medicine》2014
The Pacific oyster, Crassostrea gigas, plays a significant role in the aquaculture industry in Ireland. Episodes of increased mortality in C. gigas have been described in many countries, and in Ireland since 2008. The cause of mortality events in C. gigas spat and larvae is suspected to be multifactorial, with ostreid herpesvirus 1 (OsHV-1, in particular OsHV-1 μvar) considered a necessary, but not sufficient, cause. The objectives of the current study were to describe mortality events that occurred in C. gigas in Ireland during the summer of 2011 and to identify any associated environmental, husbandry and oyster endogenous factors. A prospective cohort study was conducted during 2010–2012, involving 80 study batches, located at 24 sites within 17 bays. All 17 bays had previously tested positive for OsHV-1 μvar. All study farmers were initially surveyed to gather relevant data on each study batch, which was then tracked from placement in the bay to first grading. The outcome of interest was cumulative batch-level mortality (%). Environmental data at high and low mortality sites were compared, and a risk factor analysis, using a multiple linear regression mixed effects model, was conducted. Cumulative batch mortality ranged from 2% to 100% (median = 16%, interquartile range: 10–34%). The final multivariable risk factor model indicated that batches imported from French hatcheries had significantly lower mortalities than non-French hatcheries; sites which tested negative for OsHV-1 μvar during the study had significantly lower mortalities than sites which tested positive and mortalities increased with temperature until a peak was reached. There were several differences between the seed stocks from French and non-French hatcheries, including prior OsHV-1 μvar exposure and ploidy. A range of risk factors relating to farm management were also considered, but were not found significant. The relative importance of prior OsHV-1 μvar infection and ploidy will become clearer with ongoing selection towards OsHV-1 μvar resistant oysters. Work is currently underway in Ireland to investigate these factors further, by tracking seed from various hatchery sources which were put to sea in 2012 under similar husbandry and environmental conditions. 相似文献
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105.
OBJECTIVES: As a part of an ongoing project to develop a nutritional screening tool, we evaluated the performance of a semi-quantitative food-frequency questionnaire (FFQ) in terms of validity in a Sheffield Caucasian pregnant population using two different statistical approaches--the correlation coefficient and the limits of agreement (LOA). The FFQ was designed specifically for pregnant women and previously used in a large-scale study. DESIGN: A validation study. SETTING: A community-based field study of a general population of pregnant women booked for their first antenatal appointment at the Jessop Wing, Royal Hallamshire Hospital, Sheffield, UK. SUBJECTS: One hundred and twenty-three women of different socio-economic status, aged between 17 and 43 years, provided complete dietary data. RESULTS: The validity of the FFQ was tested against a series of two 24-hour recalls. As expected, the intakes of all examined nutrients, except for iodine, carotene, vitamin E, biotin, vitamin C and alcohol, were higher when determined by the FFQ than when determined by 24-hour recall. Pearson's correlation coefficient between the two methods ranged from 0.19 (added sugar, zinc) to 0.47 (Englyst fibre). The LOA were broader for some of the nutrients, e.g. protein, Southgate fibre and alcohol, and an increasing lack of agreement between the two methods was identified with higher dietary intakes. CONCLUSIONS: The FFQ gave useful estimates of the nutrient intakes of Caucasian pregnant women and appears to be a valid tool for categorising pregnant women according to dietary intake. The FFQ performed well for most nutrients and had acceptable agreement with the 24-hour recall. 相似文献
106.
We analyzed longitudinal data on academic careers and conducted interviews with faculty members to determine the scope and causes of the gender gap in patenting among life scientists. Our regressions on a random sample of 4227 life scientists over a 30-year period show that women faculty members patent at about 40% of the rate of men. We found that the gender gap has improved over time but remains large. 相似文献
107.
Since the 1970s the control of bovine tuberculosis (bTB) in cattle, predominantly in the SW of England has proved continually recalcitrant; it is currently increasing at an annual rate of 18%. This deterioration has occurred despite a succession of government schemes involving killing badgers, Meles meles, with the intention of reducing transmission of bTB to cattle. Of various hypotheses proposed to explain this failure of control, some concern agricultural practice, but two concern wildlife. The latter are, first, that wild mammalian species other than badgers are important in the transmission of bTB, and second, that a perturbation effect amongst those badgers surviving a cull countermands the hoped-for reduction of bTB transmission to cattle or even increases it. We review our own studies of these two hypotheses in the context of other findings. We conclude that the other species hypothesis does not provide a general explanation for the failure of bTB control. We also conclude that the perturbation hypothesis is supported by the data and does provide one plausible mechanism to explain why culling badgers has not generally achieved control of bTB in cattle. We have reviewed the relevance of perturbation with respect to three key questions:
- (a)
- is there evidence of a perturbation effect on badger behavioural ecology?
- (b)
- is there evidence of a perturbation effect on prevalence of bTB in badgers?
- (c)
- are any observed effects of a magnitude relevant to bTB control policy?
108.
Bell JG Preston T Henderson RJ Strachan F Bron JE Cooper K Morrison DJ 《Journal of agricultural and food chemistry》2007,55(15):5934-5941
Recent legislation in the European Union (EC/2065/2001) requires that seafood must provide the consumer with information that describes geographical origin and production method. The present studies aimed to establish methods, based on chemical and stable isotopic analysis, that could reliably differentiate between wild and farmed European sea bass (Dicentrarchus labrax). The study measured fatty acid and isotopic compositions (delta13C and delta18O) of total flesh oil, delta15N of the glycerol/choline fraction, and compound-specific analysis of fatty acids (delta13C) by isotope ratio mass spectrometry. The sample set comprised 10 wild and 10 farmed sea bass from England (wild) and Scotland or Greece (farmed). Discrimination was achieved using fatty acid composition with 18:0, 18:2n-6, 20:4n-6, and 22:6n-3 providing the highest contributions for discrimination. Principal component analysis of the data set provided good discrimination between farmed and wild sea bass where factor 1 and factor 2 accounted for 60% of the variation in the data. 相似文献
109.
Shinichi Nakagawa Terry D. Bannister Fiona R. Jensen Donald A. McLean Joseph R. Waas 《Biology and Fertility of Soils》2002,35(5):390-392
Animals that can distinguish kin from non-kin may enhance their breeding success through "optimal outbreeding". Although relatedness can influence the breeding success of earthworms, no studies of kin discrimination have been conducted in any Oligochaeta species. We determined whether Eisenia fetida Sav. would discriminate kin and change breeding effort with them. The number of cocoons produced (fecundity), viable cocoons, and hatchlings produced by full sibling (inbreeding) and non-sibling (outbreeding) pairs, from different populations, were compared; in this species, fecundity is a reliable indicator of the occurrence and frequency of mating. There were no significant differences in fecundity between inbreeding and outbreeding pairs, so we obtained no evidence for differential investment based on kinship. However, sibling pairs hatched a significantly greater proportion of their cocoons than non-sibling pairs, suggesting that outbreeding depression may reduce reproductive success. We also provide the first evidence for the possibility of parthenogenesis in this species. 相似文献
110.
VORTEX computer simulations were used to assess the viability of a small breeding flock of common cranes, recently established in Norfolk. Investigations were also conducted on the relative impact of various management options. Raising the carrying capacity of the site had little influence on the survival of the flock. However, great improvements were associated with increases in the size of the founder flock and reductions in the mortality rates of chicks and adults. Regularly supplementing the population increased flock survival times: for a fixed total number of birds added, frequently supplementing with few birds produced the best results. Immigration from the population in continental Europe also increased persistence of the flock, and it is possible that immigration rates will improve as the British flock expands. Strategies including captive breeding, and regular supplementation of the flock, may be the most effective means of ensuring the viability of the Norfolk cranes. The potential value of simulation models in guiding management of small populations is discussed. 相似文献