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11.
Weeds PL Chakraborty S Fernandes CD d'A Charchar MJ Ramesh CR Kexian Y Kelemu S 《Phytopathology》2003,93(2):176-185
ABSTRACT Using molecular markers, this work compares the genetic diversity in Colletotrichum gloeosporioides infecting species of the tropical forage legume Stylosanthes at the center of origin in Brazil and Colombia with that of Australia, China, and India, where Stylosanthes spp. have been introduced for commercial use. There was extensive diversity in the pathogen population from Brazil, Colombia, China, and India. The Australian pathogen population was least diverse probably due to its geographical isolation and effective quarantine. The extensive diversity in China and India means that threats from exotic pathogen races to Stylosanthes pastures can potentially come from countries outside the South American center of origin. In Brazil and India, both with native Stylosanthes populations, a high level of genetic differentiation in the pathogen population was associated with sites where native or naturalized host population was widely distributed. There was limited genetic diversity at germplasm evaluation sites, with a large proportion of isolates having identical haplotypes. This contrasts recent pathogenicity results for 78 of the Brazilian isolates that show hot spots of complex races are more common around research stations where host germplasm are tested, but few are found at sites containing wild host populations. For a pathogen in which the same races arise convergently from different genetic backgrounds, this study highlights the importance of using both virulence and selectively neutral markers to understand pathogen population structure. 相似文献
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Paranhos-Silva M Oliveira GG Reis EA de Menezes RM Fernandes O Sherlock I Gomes RB Pontes-de-Carvalho LC dos-Santos WL 《Veterinary parasitology》2003,114(2):97-111
In this study, we compare the development of infection and/or disease in Beagle dogs intradermally infected with Leishmania chagasi, in the presence or absence of Lutzomyia longipalpis saliva, with those of intravenously infected animals.Spleen samples of all the animals inoculated with parasites had positive polymerase chain reaction tests for Leishmania DNA. Positive spleen cultures for Leishmania were detected earlier (P < or = 0.018) and were more frequent (five out of the five animals) in intravenously infected animals than in the intradermally infected animals, in presence (two out of the six animals) or absence (three out of the five animals) of salivary gland lysate of L. longipalpis. Significant increase in serum antibodies against Leishmania was observed only in the intravenously infected group (P = 0.004). In addition, dogs with infection confirmed by isolation of amastigotes or detection of parasite DNA were, nevertheless, negative for anti-Leishmania antibodies up to 5 months or more after infection. Only animals of the intravenously infected group developed progressive decreases in hematocrit (Pearson r = -0.8076, P = -0.0026) and hemoglobin (Pearson r = -0.8403, P = 0.0012) during the infection period. No significant difference in the course of infection was observed between groups of intradermally infected animals. The data presented herein confirms that the intradermal inoculation of dogs with Leishmania produces an asymptomatic form of infection. It also fails to show an advantage in using L. longipalpis saliva as an infection-enhancing agent in experimental canine leishmaniasis. 相似文献
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B.A.N. Silva R.F.M. Oliveira J.L. Donzele H.C. Fernandes M.L.T. Abreu J. Noblet C.G.V. Nunes 《Livestock Science》2006,105(1-3):176-184
Forty Landrace × Large White lactating sows were used to evaluate the effects of cooling of the floor when maintained under high temperature conditions during summer on their productive and reproductive performance. The sows were allocated in a completely randomized design with two treatments with 20 replicates according to parity number and body weight, with each animal being considered an experimental unit. The treatments consisted of cooling of the floor under the sow with water circulation at about 17 °C and no cooling. The resulting temperatures of the floor were 27.6 and 35.8 °C for the cooled and the control treatments, respectively. The sows from both treatments were exposed to average maximum and minimum environmental temperatures of 26.9 and 20.8 °C, respectively. Sows maintained on a cooled floor had a higher feed intake (6.47 vs. 5.61 kg/day; P < 0.01). Despite this higher intake, sows maintained on a cooled floor had higher body weight and body protein losses during the lactation period (P < 0.01) in connection with a higher milk yield and subsequent growth of the litter (2280 vs. 1798 g/day; P < 0.01). There was an effect of treatment on rectal temperature, surface temperatures and respiratory rate (P < 0.01) with lower values in sows submitted to floor cooling. It is concluded that floor cooling under the lactating sow improves its productive and reproductive performance, as well as the performance of its litter. 相似文献
15.
Fabrício de Oliveira Reis Eliemar Campostrini Elias Fernandes de Sousa Marcelo Gabetto e Silva 《Scientia Horticulturae》2006
In papaya plants a study to quantify the water flow through the trunk is important for to promote a good water management in commercial orchard. The objective was to study the relationship between water flow through the trunk and temperature measurements determined by probes inserted in the papaya plant stem in laboratory. In addition, was possible to study the relationship between sap flow and instantaneous gas exchange in field conditions. We constructed an instrument that maintained a stable water flux through a 0.30 m stem section with a constant pressure, simulating the xylem sap flow through the stem. Water flux was adjusted by varying pressure of water in the stem section. The mathematical model used to fit the relation between K (Granier heat coefficient) values and sap flow density was the exponential model: u = 0.5511 × K1.9104. Field studies was conducted in a commercial orchard located in North of the State of Rio de Janeiro, with 12 plants in October 2002, and eight plants in January 2003. We verified that instantaneous transpiration, measured by a portable system of gas exchange (porometry), presented a good (R2 = 0.75) positive relationship with xylem sap flow. Estimates of papaya sap flow can be obtained by scaling portable photosynthesis system measurements with exposed leaves, however the relationship is non-linear in higher instantaneous transpiration rates. The causes of the non-linear relationship in higher transpiration are discussed. In addition, was possible to obtain a good (R2 = 0.76) relationship between net photosynthesis rate and xylem sap flow in papaya field-grown. 相似文献
16.
Thaís C.C. Fernandes 《Pesticide biochemistry and physiology》2007,88(3):252-259
The trifluralin is an agent that promotes a cellular damage due to its direct action on the microtubules. This action leads to a decontrol in the cellular division, bringing about polyploid cells. In this work, we show the evidences that the exceeding genetical material of theses polyploidizated cells tends to be eliminated from the nucleus in the form of micronucleus. Our analyses prove this fact, both by the presence of a number of cells carrying micronucleus, and by the evidences of the elimination of the exceeding material itself, after exposition of the Allium cepa root tips tested with several concentration of trifluralin herbicide. It was noticed that the residual concentration induced a number of polyploid cells, micronuclei and mini cells. Inferences about the implications of the elimination of genetic material from micronuclei, such as cell viability and apoptosis, are also presented. 相似文献
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The homegardens are characterised by an intensive integration of numerous multipurpose trees and shrubs with food crops and animals, simultaneously on the same unit of land.The Chagga are skilled farmers with an intimate knowledge of the crops and their ecological requirements. They have a good idea of functions/uses of the plant species on their farms. The large species diversity provides both subsistence and cash crops. It enables the farmer to keep his management options open and provides insurance against drought, pest and economic risks. 相似文献
20.
The Forest village scheme was introduced by the Forest Industries Organisation (FIO) of Thailand in 1967 as an attempt to stop further spread of the fast increasing shifting cultivation and deforestation in the country. The underlying princple of the scheme is to relate reforestation with social welfare of the people involved. It is essentially a modification of the traditional taungya method of plantation establishment.The salient aspect of the scheme is to induce the shifting cultivators to settle down in villages where each family is given tenure over a plot of land to construct a house and develop a homegarden around it. The farmers are required to help establish and maintain forest plantations, in which they are permitted to raise agricultural crops during the first three years of its establishment. The farmers are also given free medical and educational facilities, and technical advice on crop and livestock husbandry. They can also earn cash rewards for successful plantation establishment.Although the scheme has not achieved its full target in terms of area covered and number of families settled, it is proving to be a successful method of luring people away from destructive shifting cultivation. The approach is applicable to other countries and regions with similar land-use problems and socio-cultural background.The paper also examines the constraints to the effective working of the scheme, provides some simple suggestions for improving its functioning and identies some of the issues that can easily be tackled by research. 相似文献