Effects of Different Types of Composts on Soil Characteristics and Morphological Traits of Two Dry Rangeland Species

Today, environmental protection and safe crop production are very important. The management of soil elements by compost is considered as important for sustainable agriculture. The mode of action of the composts is very different between various plant species. To evaluate the effects of different composts on soil structural and chemical properties and on morphological traits of two dry rangeland species (atriplex; Atriplex lentiformis and mesquite; Prosopos juliflora), a study was conducted in Fars Province of Iran during the year 2010. The study was a factorial experiment based on a randomized complete block design with three replications. Treatments included compost types: solid (SC) and liquid compost or compost tea (LC), solid-liquid mixture (XSL) and control (Con; non used compost) as the first factor, and two pasture plant species as the second factor. The results showed that the compost application had significant and positive effects on morphological traits such as plant height, stem diameter, plant volume, crown length, width, and area, and caused 15, 51.18, 70.67, 34.18, 18.35, and 64.94% increase on these morphological traits, respectively. Although soil acidity was not significantly affected by compost and species, the effects of compost were significant on organic matter percentage, soil phosphorous, and potassium contents. Soil nitrogen percentage was affected by both species and compost. Compost application caused a decrease in the amount of sodium compared with the control. Overall, the results of this study suggested that within the compost types, liquid compost was an advisable biofertilizer in a similar climate. Furthermore, the LC and the XSL are recommended for improving the morphological traits and the soil characteristics, respectively.     

EFFECTS OF PHYTOHORMONES ON PROLINE CONTENT AND ANTIOXIDANT ENZYMES OF VARIOUS WHEAT CULTIVARS UNDER SALINITY STRESS

Salinity stress is one of the important agricultural problems in the world. A factorial experiment based on completely randomized design with four replications was conducted to evaluate the effects of phytohormones (gibberellic acid and abscisic acid) on the activity of antioxidant enzymes (peroxidase, superoxide dismutase and catalase), rubisco activity and content, and proline in three wheat cultivars (Gascogen, Zagros, and Kuhdasht) under control and salinity stress (3.5 and 7 dS m^?1). The results showed that salinity stress (3.5 and 7 dS m^?1) decreased the activity of catalase, rubisco, carboxylase, but increased peroxidase, superoxide dismutase activity and proline content. Gibberellic acid caused 58.03% increased in rubisco carboxylase activity in Zagros at 7 dS m^?1 in comparison with abscisic acid under salinity stress compared with the control plants in Kuhdasht. Activity of superoxide dismutase in Kuhdasht cultivar at 7 dS m^?1 salinity level showed 76.43% increased in Gascogen under salinity stress compared with the control plants with gibberellic acid application. The highest proline content as an osmolyte was found in Zagros at 7 dS m^?1 salinity level with abscisic acid (194 μmol g^?1 DM) application. Peroxidase activity increased 83.31% and catalase activity decreased 61.27% compared with the control plants in Zagros. Gibberellic acid application significantly prevented reduction in rubisco content under salinity stress. In conclusion, increased in peroxidase and superoxide dismutase activity and proline content decreased the adverse effects of salinity stress on studied cultivars. Also, the foliage spray of gibberellic acid enhanced and improved the growth condition. In this experiment, Zagros cultivar showed more tolerance to salinity stress than the other two cultivars.     

Potassium release from sand,silt and clay fractions in calcareous soils of southern Iran

The release of non-exchangeable potassium from 24 calcareous soils of divergent mineralogy, from southern Iran, was examined. Sand, silt and clay particles were fractionated after dispersion with an ultrasonic probe. Samples were extracted with 0.01 M CaCl₂ for 30 successive 2-h periods. The clay fraction released the largest amount of K in each soil. Cumulative K released ranged from 175 to 723, 35 to 128, and 71 to 146 mg kg^?1 contributing 20–90, 4–39 and 2–54% for clay, silt and sand fractions, respectively. The lower proportion of K released from sand and silt fractions can be explained by the presence of a high content of CaCO₃ and quartz in these fractions. The release kinetics for the non-exchangeable K data showed that parabolic diffusion and power function were the best fitting kinetic models. This indicated that slow diffusion of K from the mica interlayer positions is the main rate-controlling process. Cumulative K released and constant b values of parabolic diffusion model correlated significantly with the mica content of the clay fraction.     

Calibration and validation of four common ET0 estimation equations by lysimeter data in a semi-arid environment

In this study, four different methods for reference crop evapotranspiration (ET₀) were calibrated and validated for estimation of daily to mean monthly ET₀ by weighing lysimeter data during 2005–2006 and 2004–2005, respectively, in a semi-arid region. The value of the constant in the Hargreaves–Samani method changed from 0.0023 to 0.0026 for daily to mean monthly ET₀, and can be used in stations with only air temperature data. The constant of the aerodynamic resistance equation in the FAO-56 Penman–Monteith method (208.0) changed to 85.0. The value of coefficient a in the FAO-24-Radiation method was between ?0.5 and ?0.67. Further, the empirical equations were modified to estimate the value of b in the FAO-24-Radiation method and C in the FAO-24 corrected Penman method. The results showed that the modified FAO-56, corrected Penman–Monteith and FAO-24-Radiation methods are the most appropriate for estimating daily to mean monthly ET₀. Furthermore, the modified FAO-24 corrected Penman method was ranked in fourth place and its accuracy was lower than that of the other methods. However, it is appropriate for estimating mean monthly ET₀. Smoothing the daily data decreased the fluctuation in measured daily weather data and ET₀ measured by lysimeter, and consequently resulted in a higher accuracy in the estimation of daily ET₀.     

Development of a monoclonal immunoassay selective for chlorinated cyclodiene insecticides

Organochlorine pesticides still generate public health concerns because of their unresolved health impact and their persistence in living beings, which is demanding appropriate analytical techniques for their monitoring. In this study, an enzyme-linked immunosorbent assay based on monoclonal antibodies (MAbs) for the detection of an important group of organochlorine pesticides, the cyclodiene group, has been developed. With this aim, several hapten-protein conjugates, characterized by exposure of the common hexachlorinated bicyclic (norbornene) moiety and differing in the linking structure to the carrier protein, were prepared. From mice immunized with these conjugates, several MAbs with the ability to sensitively bind the majority of cyclodienes were obtained. Among them CCD2.2 MAb displaying the broadest recognition to cyclodiene compounds (endosulfan, dieldrin, endrin, chlordane, heptachlor, aldrin, toxaphene: I(50) values in the 6-25 nM range) was selected for the assay. Interestingly, this MAb showed certain stereospecificity toward other polychlorinated cycloalkanes because the gamma-isomer of hexachlorocyclohexane (lindane) was also very well recognized (I(50) value of 22 nM). This immunoassay is potentially a very valuable analytical tool for the rapid and sensitive determination of cyclodiene insecticides and related compounds, which in turn may contribute to the understanding of the biological activities and of the overall environmental impact of these persistent organic pollutants.     

Projected impacts of global warming on cropping calendar and water requirement of maize in a humid climate

In this research, the influence of climate change on maize cultivation was investigated and then, the possible solutions for adopting this natural hazard in the coasts of Caspian Sea in Iran was assessed. Weather data were generated for the 2011–2100 period using a statistical downscaling model under different climatic scenarios. Reference evapotranspiration (ETo) was calculated using a Neuro-fuzzy inference system. Cop water requirement was calculated by multiplying ETo by crop coefficients. Increased cardinal temperatures during 2011–2100 led to shifting in the planting date backward by 10–26 days. In addition, the projected global warming has a considerable effect on the duration of the vegetative growth stage resulting in earlier harvesting. However, the duration of the reproductive stage is less affected. Despite the obvious reduction in the length of the growing season, crop water requirement will increase by 10.6–15.3% in the future due to 1.64–28.4% increase in ETo. However, changing the cultivation time may lead to 11.2–264.5 m³ ha^?1 water saving during the whole cropping cycle through affecting both ETo and the crop growth cycle. This result demonstrates that management of the maize cropping calendar can be an effective way to achieve sustainable agriculture under future climate conditions in the study area.     

Soil seed banks in the Arasbaran Protected Area of Iran and their significance for conservation management

The Arasbaran Protected Area is an important but little studied nature reserve in NW Iran. In the past habitat heterogeneity and biodiversity have been threatened by over-exploitation; in the future depopulation may lead to partial abandonment. The soil seed bank was examined to see whether there are sufficient species represented as persistent seeds to reconstitute the vegetation in the event of habitat deterioration. There were differences between seed banks from different soils and vegetation types but essentially all soil seed banks contained few species and low densities of seed. Moreover, the species in the seed bank were unrepresentative of the vegetation. During future periods of changing land use it will be important to maintain the existing vegetation. There are however indications of successful recent conservation management. Annuals are abundant in the woodland seed bank but absent from the vegetation. The forest is recovering from the disturbance of collecting fire-wood.     

Investigation on the Biodegradability and Antibacterial Properties of Nanohybrid Suture Based on Silver Incorporated PGA-PLGA Nanofibers

Polyglycolic acid-poly lactic glycolic acid (PGA-PLGA) electrospun nanofibers containing silver nanoparticles have been produced and twisted into the nanofibrous yarn. The morphology of nanofibers and produced yarns, as well as the mechanical properties of the yarns, were investigated. Furthermore, in vitro antibacterial properties and in vitro degradation behavior of yarns containing various silver nanoparticles were studied. SEM images confirmed that the addition of the silver nanoparticles into the polymer solution increases the fiber diameters. The result of the mechanical test of the yarns alone and used in two different forms of the knots was measured and results showed that the strength of the yarns without the knot was significantly more than that of others. The biodegradability test showed that the mechanical properties and the weight of the yarns were quickly reduced after subjecting to in vitro condition. The result of the antibacterial test indicated that the nanofiber yarns containing %3 silver nanoparticles were the most appropriate sample with a considerably antibacterial activity against both gram-positive bacterium Staphylococcus aureus and gram-negative bacterium Escherichia Coli with inhibition zones of 8.1 and 9.5 mm, respectively; which demonstrated that silver nanoparticles retained their effectiveness after the electrospinning process. Therefore the nanofibrous yarns containing silver nanoparticles could be successfully produced by the electrospinning process with the proper antibacterial property as a candidate for the surgical sutures.     

Fabrication of Biocompatible PLGA/PCL/PANI Nanofibrous Scaffolds with Electrical Excitability

Application of electrospun nanofibrous scaffolds has received immense attention in tissue engineering. Fabrication of scaffolds with appropriate electrical properties plays a key role in neural tissue engineering. Since fibers orientation in the scaffolds affects the growth and proliferation of the cells, this study aimed to prepare aligned electrospun conductive nanofibers by mixing 1 %, 10 % and 18 % (w/v) doped polyaniline (PANI) with polycaprolactone (PCL)/poly lactic-coglycolic acid (PLGA) (25/75) solution through the electrospinning process. The fibers diameter, hydrophilicity and conductivity were measured. In addition, the shape and proliferation of the nerve cells seeded on fibers were evaluated by MTT cytotoxicity assay and scanning electron microscopy. The results revealed that the conductive nanofibrous scaffolds were appropriate substrates for the attachment and proliferation of nerve cells. The electrical stimulation enhanced neurite outgrowth compared to those PLGA/PCL/PANI scaffolds that were not subjected to electrical stimulation. As polyaniline ratio increases, electric stimulation through nanofibrous PLGA/PCL/PANI scaffolds results in cell proliferation enhancement. However, a raise more than 10 % in polyaniline will result in cell toxicity. It was concluded that conductive scaffolds with appropriate ratio of PANI along with electrical stimulation have potential applications in treatment of spinal cord injuries.     

Expression of the herpes simplex virus type 2 glycoprotein D in baculovirus expression system and evaluation of its immunogenicity in guinea pigs

Background: Herpes simplex virus type 2 (HSV-2) is highly prevalent and major cause of genital herpes in humans. The life-long nature of infection and the increasing prevalence of genital herpes imply that vaccination is the best strategy for controlling the spread of infection and limiting HSV disease. HSV glycoprotein D (gD) is one of the most important viral immunogen which has an essential role in virus infectivity and induction of immune responses. Methods: HSV-2 DNA was extracted and used as template in polymerase chain reactions to amplify gD2 gene. The PCR product was confirmed by restriction enzyme analysis, cloned into a cloning vector and then sequenced. The Bac-to-Bac expression system was used to express HSV-2 gD in insect cells. The expressed protein was used as subunit vaccine to immunize guinea pigs after confirmation. Results: The expressed protein was confirmed with SDS-PAGE and Western-blot analysis. In Western-blot analysis, two major protein bands, with approximate molecular weights of 52-55 and 41-43 kDa corresponding to the glycosylated and non-glycosylated forms of gD2 protein, were observed, respectively. Immunization with the recombinant gD2 could elicit humoral responses in guinea pigs as measured by neutralization test and ELISA, and offered high protection against induced HSV-2 genital disease. Conclusion: The baculovirus expression of heterologous genes permits proper folding, post-translational modification and oligomerization in manners that are often identical to those that occur in mammalian cells. Expression of proteins under the control of the strong polyhedrin promoter, allowing high level protein production, can be used as subunit vaccine.