A field study of gross rates of N mineralization and nitrification and their relationships to microbial biomass and enzyme activities in soils treated with dairy effluent and ammonium fertilizer

Abstract. Gross N mineralization and nitrification rates were measured in soils treated with dairy shed effluent (DSE) (i.e. effluent from the dairy milking shed, comprising dung, urine and water) or ammonium fertilizer (NH₄Cl) under field conditions, by injecting ¹⁵N-solution into intact soil cores. The relationships between gross mineralization rate, microbial biomass C and N and extracellular enzyme activities (protease, deaminase and urease) as affected by the application of DSE and NH₄Cl were also determined. During the first 16 days, gross mineralization rate in the DSE treated soil (4.3–6.1 μg N g^?1 soil day^?1) were significantly (P 14;< 14;0.05) higher than those in the NH₄Cl treated soil (2.6–3.4 μg N g^?1 soil day^?1). The higher mineralization rate was probably due to the presence of readily mineralizable organic substrates in the DSE, accompanied by stimulated microbial and extracellular enzyme activities. The stable organic N compounds in the DSE were slow to mineralize and contributed little to the mineral N pool during the period of the experiment. Nitrification rates during the first 16 days were higher in the NH₄Cl treated soil (1.7–1.2 μg N g^?1 soil day^?1) compared to the DSE treated soil (0.97–1.5 μg N g^?1 soil day^?1). Soil microbial biomass C and N and extracellular enzyme activities (protease, deaminase and urease) increased after the application of the DSE due to the organic substrates and nutrients applied, but declined with time, probably because of the exhaustion of the readily available substrates. The NH₄Cl application did not result in any significant increases in microbial biomass C, protease or urease activities due to the lack of carbonaceous materials in the ammonium fertilizer. However, it did increase microbial biomass N and deaminase activity. Significant positive correlations were found between gross N mineralization rate and soil microbial biomass, protease, deaminase and urease activities. Nitrification rate was significantly correlated to biomass N but not to the microbial biomass C or the enzyme activities. Stepwise regression analysis showed that the variations of gross N mineralization rate was best described by the microbial biomass C and N.     

Mulching Improves Water Productivity,Yield and Quality of Fine Rice under Water‐saving Rice Production Systems

Water‐saving rice production systems are inevitable in the wake of severe water shortage in rice‐growing regions of the world. Mulches can improve water productivity, yield and quality of rice through increase in water retention. Studies were conducted for two consecutive years to assess the potential role of mulches (plastic and straw) in improving the performance of water‐saving rice production systems in comparison with no mulch used and conventionally irrigated transplanted rice. Water‐saving rice production systems in this study comprise aerobic rice and transplanted rice with intermittent irrigation. These systems saved water (18–27 %) with improved water productivity more than the conventional system. However, these systems caused a yield penalty of 22–37 %; nevertheless, these yield losses were compensated with the application of mulches under water‐saving rice production systems. Both plastic and straw mulches were helpful in improving moisture retention and water productivity (0.18–0.25 kg grain m^?3 water) relative to non‐mulch treatments (0.19–0.29 kg grain m^?3 water). Mulch application was also helpful in reducing the number of non‐productive tillers and sterile spikelets, and improving the productive tillers, kernel number and size, and kernel quality. Plastic mulch was more effective than straw mulch in improving water retention, water productivity and reducing spikelet sterility. In conclusion, the mulching improved the soil moisture retention, and thus enhanced the rice water productivity, spikelet fertility, paddy yield and quality of rice. This signifies the importance of mulching in water‐saving rice production systems.     

Organic Nitrogen Source Addition for Improving the Physicochemical Properties of Sandy Loam Soil and Maize Performance

Poor quality of sandy loam soils ?is the main reason for low crop yield. Improvement of physicochemical properties of these soils is very challenging. Addition of organic sources may improve the soil properties. Therefore, this study investigated the adequacy of poultry-manure-compost (PMC) and pressmud-compost (PrMC) at 0 (control), 2, 4, 6, 8, and 10 t ha^?1 for improving the physicochemical properties of sandy loam soil and maize performance. An increasing trend in most soil and crop traits was seen with increasing compost levels. For 10 t PMC ha^?1, soil inorganic-N (512%), organic-carbon (78%), and water-holding capacity (65.36%) improved maximum. This resulted in the maximum mean crop growth rate (43.85%), stover yield (94%), grain protein (21%), and nitrogen use efficiency (30.6 kg kg^?1). Contrarily, grain oil (?7%) was lowest at 10 t PMC ha^?1. Consequently, 10 t PMC ha^?1 could be much effective to improve the physicochemical properties of sandy loam soils and maize performance.     

Nitrous oxide emissions from a rainfed-cultivated black soil in Northeast China: effect of fertilization and maize crop

Nitrous oxide emission (N₂O) from applied fertilizer across the different agricultural landscapes especially those of rainfed area is extremely variable (both spatially and temporally), thus posing the greatest challenge to researchers, modelers, and policy makers to accurately predict N₂O emissions. Nitrous oxide emissions from a rainfed, maize-planted, black soil (Udic Mollisols) were monitored in the Harbin State Key Agroecological Experimental Station (Harbin, Heilongjiang Province, China). The four treatments were: a bare soil amended with no N (C0) or with 225?kg?N ha^?1 (CN), and maize (Zea mays L.)-planted soils fertilized with no N (P0) or with 225?kg?N ha^?1 (PN). Nitrous oxide emissions significantly (P?<?0.05) increased from 141?±?5?g N₂O-N?ha^?1 (C0) to 570?±?33?g N₂O-N?ha^?1 (CN) in unplanted soil, and from 209?±?29?g N₂O-N?ha^?1 (P0) to 884?±?45?g N₂O-N?ha^?1 (PN) in planted soil. Approximately 75?% of N₂O emissions were from fertilizer N applied and the emission factor (EF) of applied fertilizer N as N₂O in unplanted and planted soils was 0.19 and 0.30?%, respectively. The presence of maize crop significantly (P?<?0.05) increased the N₂O emission by 55?% in the N-fertilized soil but not in the N-unfertilized soil. There was a significant (P?<?0.05) interaction effect of fertilization?×?maize on N₂O emissions. Nitrous oxide fluxes were significantly affected by soil moisture and soil temperature (P?<?0.05), with the temperature sensitivity of 1.73–2.24, which together explained 62–76?% of seasonal variation in N₂O fluxes. Our results demonstrated that N₂O emissions from rainfed arable black soils in Northeast China primarily depended on the application of fertilizer N; however, the EF of fertilizer N as N₂O was low, probably due to low precipitation and soil moisture.     

Municipal Solid Waste Compost Improves Crop Productivity in Saline-Sodic Soil: A Multivariate Analysis of Soil Chemical Properties and Yield Response

A pot experiment was conducted in sandy clay loam saline-sodic soil to assess the effects of farm yard manure (FYM), municipal solid waste (MSW) composts and gypsum application on nitrate leaching, soil chemical properties and crop productivity under rice-wheat cropping system. It also aims at establishing the correlation between soil phsico-chemical properties and yield response using principle component analysis and Pearson correlation analysis. The MSW was decomposed aerobically, an-aerobically and co-composted. Maximum nitrate leaching was observed during rice (75.9 mg L^?1) and wheat (37.2 mg L^?1) with an-aerobically decomposed MSW as compared with control treatment. Results revealed a decrease in soil pH (?6.95% and ?8.77%), electrical conductivity (EC) (?48.13% and ?51.04%), calcium carbonate (CaCO₃) (?40.30% and ?48.96%), and sodium adsorption ratio (SAR) (?40.27% and ?45.98%) with an-aerobically decomposed MSW compost during rice and wheat, respectively. In this treatment, organic matter (OM) (93.55% and 121.51%) and cation exchange capacity (CEC) (19.31% and 31.79%) were the highest as compared with control treatment during rice and wheat, respectively. Rice and wheat growth were significantly (p≤ 0.05) increased by an-aerobically decomposed MSW followed by co-compost, aerobically decomposed MSW, FYM, gypsum and control. Furthermore, Pearson correlation coefficients predicted significant positive correlation of yield with soil OM, and CEC while inverse relationship was observed with EC, pH, CaCO₃, and nitrogen use efficiency. Soil amelioration with organic and gypsum amendments was further confirmed with principal component analysis. This study has proved an-aerobically decomposed MSW as an effective solution for MSW disposal, thereby improving soil chemical properties and crop productivity from sandy clay loam saline-sodic soil.     

Constituents and cytotoxicity of Zanthoxylum rhesta stem bark

3,5-Dimethoxy-4-geranyloxycinnamyl alcohol (1), 8-methoxy-N-methylflindersine (2), xanthyletin and sesamin have been isolated from petroleum ether extract of the stem bark of Zanthoxylum rhesta. The petroleum ether extract and 8-methoxy-N-methylflindersine showed cytotoxicity on brine shrimp nauplii.     

细虫草资源研究现状及开发利用进展

细虫草(Ophiocordyceps gracilis)是一种虫生真菌,为传统的哈萨克民族药,一直以来被作为冬虫夏草的替代品使用,具有重要的食用和药用价值。本文从分布、生境、形态特征、系统发育分类、生物活性成分、药理作用、人工培养菌丝体及寄主昆虫等方面对细虫草的研究进展进行总结,并对其研究前景进行了展望,为今后细虫草的研究和开发利用提供参考。     

Use of Biochar as an Amendment for Remediation of Heavy Metal-Contaminated Soils:Prospects and Challenges

Biochar (BC), known as the new black gold, is a stable, novel carbonaceous by-product that is synthesized through pyrolysis of biological materials in the absence of O₂. Recently, an emerging interest in the application of BC as a robust soil amendment has given rise to a broad research area in science and technology. It is considered a promising remediation option for heavy metal (HM)-contaminated soils to reduce HM bioavailability to plants. Remediation efficacy of BC depends on the porosity, composition, pyrolysis temperature, feedstock, and residence time of pyrolysis. This review article aimed to present an overview of BC use in the immobilization of HMs, i.e., Cd, As, Pb, Zn, Ni, Cu, Mn, Cr, and Sb, in contaminated soils. The remaining uncertain factors, including the specific soil HM immobilization mechanisms, long-term beneficial effects, and potential environmental risks associated with BC application are analyzed. Future research must be conducted to ensure that the management of environmental pollution is in accord with ecological sustainability and adaptation of the black gold biotechnology on a commercial basis for immobilization of HMs in contaminated soils.     

Molecular fingerprinting of hybrids and assessment of genetic purity of hybrid seeds in rice using microsatellite markers

Microsatellite markers were used for fingerprinting of hybrids, assessing variation within parental lines and testing the genetic purity of hybrid seed lot in rice. Ten sequence tagged microsatellite sites (STMS) markers were employed for fingerprinting 11 rice hybrids and their parental lines. Nine STMS markers were found polymorphic across the hybrids and produced unique fingerprint for the 11 hybrids. A set of four markers (RM 206, RM 216, RM 258 and RM 263) differentiated all the hybrids from each other, which can be used as referral markers for unambiguous identification and protection of these hybrids. Cluster analysis based on Jaccard's similarity coefficient using UPGMA grouped the hybrids into three clusters. Within the cluster all the hybrids shared a common cytoplasmic male sterile line as female parent. The genetic similarity between the hybrids ranged from 0.33 to 0.92 with an average similarity index of 0.63. The analysis of plant-to-plant variation within the parental lines of the hybrid Pusa RH 10, using informative markers indicated residual heterozygosity at two marker loci. This highlights the importance of STMS markers in maintaining the genetic purity of the parental lines. The unique value of the restorer gene linked marker for testing the genetic purity of hybrid seeds is demonstrated for the first time.