从江县柑桔矢尖蚧发生特点及主要因子分析

对近几年从江地区柑桔矢尖蚧(Uaspis yanonensis(kuwana))发生危害的情况和特点进行了总结和分析,指出管理不善、不易抓住防治时机、虫源和天敌是影响柑桔矢尖蚧发生的主要因子,并提出了相应的防治对策。     

马立克氏病毒单克隆抗体杂交瘤细胞株的建立与鉴定

以马立克氏病毒814株(MDV-814)作为免疫用抗原,建立了2个MDV特异性单克隆抗体(McAb)杂交瘤细胞株—4A6、3D7。鉴定结果表明;两株细胞所分泌的McAb均为IgM类免疫球蛋白,具有MDVⅠ型病毒特异性,无中和反应特性和沉淀反应特性。利用两种McAb对国内标准强毒MDV-京1株进行鉴定,肯定其为MDV-Ⅰ型毒株。     

茄子早熟育种研究Ⅱ选择指数与早熟性选择

研究结果表明,由性状果实最大周长,果实体积,门茄显蕾期,单株产量等性状构成的选择指数较好;进一步进行遗传相关分析结果认为,应用选择指数时应注意果实体积这个性状,单株产量是一个重要的辅助性状,门茄显蕾期是一个限制性因子。应用选择指数对品种评价结果认为,早熟性较好的品种有:天津早茄、西安绿茄、二民、七叶茄、白线茄和汉中荷包。     

白跗平腹小蜂对主要森林害虫抑制力研究

１９８７～１９９２年连续６年研究结果表明，白跗平腹小蜂Ａｎａｓｔａｔｕｓａｌｂｉｔａｒｓｉｓ对马尾松毛虫Ｄｅｎｄｒｏｌｉｍｕｓｐｕｎｃｔａｔｕｓ、柳杉毛虫Ｄｅｎｄｒｏｌｉｍｕｓｌａｔｉｐｅｎｎｉｓ、水青蛾Ａｃｔｉａｓｓｅｔｅｎｅｎｉｎｇｐｏａｎａ等１３种主要森林害虫卵有较明显的抑制作用。在林间，该蜂对马尾松毛虫第１、２代卵的自然抑制力分别力１６．５５％和２２．２９％，对柳杉毛虫越冬代卵的自然抑制力为２３．４５％；对水青蛾第１、２代卵的抑制力分别力５．４９％和２．６３％；在林间，人为释放白跗平腹小蜂对第１代马尾松毛虫卵的校正抑制力为３３．５％，在室内１２．３～２８℃范围内，每头雌峰对马尾松毛虫、水青蛾和柞蚕剖腹卵的日破坏数（寄生数）分别力１５、１８和２６粒，一生破坏数分别为５６、９１和２０８粒。即使虫卵即将孵化，该峰同样亦能寄生，并羽化出正常的子代白跗平腹小蜂。     

Pasteurella multocida toxin induces IL-6, but not IL-1 alpha or TNF alpha in fibroblasts.

Pasteurella multocida toxin (PMT) is the major virulence factor in Progressive Atrophic Rhinitis of swine. Other workers' previous findings that PMT was mitogenic for 3T3 fibroblasts, were confirmed in the present study. In addition, PMT stimulated 3T3 cells to release IL-6, but IL-1 alpha or TNF alpha were not detected in fibroblast supernatants sampled 24, 48, or 72 h after stimulation. In view of the role of IL-6 in osteoclastic bone resorption, these findings provide a new working hypothesis for investigations into the molecular pathogenesis of this important disease.     

叶面喷施丰产素在黄瓜上的应用效果

本试验在黄瓜上叶面喷施丰州牌丰产素，其浓度分别为２０００倍、４０００倍和６０００倍。试验结果表明，３种浓度对黄瓜的生长势都有增强的作用，并对早期产量有较大的提高。其中２０００倍液对早期产量的增产幅度最大，比对照提高４２．８１％。并且对黄瓜的营养成份有不同程度的影响，其中６０００倍液对维生素ｃ和含糖量都有较大的提高，因此，对黄瓜品质的改进有较好的效果。     

Comparison of dot blot hybridization, polymerase chain reaction, and virus isolation for detection of bovine herpesvirus-1 (BHV-1) in artificially infected bovine semen.

Bovine semen samples spiked with bovine herpesvirus 1 (BHV-1) were used to compare dot blot hybridization, polymerase chain reaction (PCR), and virus isolation for detection of BHV-1 in bovine semen. The PCR amplification used primers targeting the BHV-1 thymidine kinase gene and a nucleic acid releasing cocktail (GeneReleaser); the PCR product was used as the DNA probe in dot blot hybridization; virus isolation was done in primary bovine fetal testis (BFT) cell cultures. Semen diluted 1:20 in tissue culture medium had the least cytotoxicity and inhibition of viral cytopathic effects in BFT cells, allowing detection of 1 TCID50/100 microL of BHV-1 suspension by virus isolation. The presence of foreign DNA such as bovine sperm DNA or salmon sperm DNA increased the sensitivity of dot blot hybridization in detecting BHV-1, allowing detection of 20,000 TCID50/100 microL of neat semen. The inhibition of PCR amplification of BHV-1 DNA in bovine semen was eliminated by diluting the samples 1:20 in tissue culture medium. The best PCR amplification was obtained when semen was diluted 1:20 and when a reaction buffer of pH 9.0, with 1.0 mM MgCl2 was used. Under these conditions, the PCR followed by ethidium bromide staining of agarose gels could detect 1 TCID20/100 microL of sample, whereas PCR followed by Southern blot hybridization could detect 0.01 TCID50/100 microL of sample.(ABSTRACT TRUNCATED AT 250 WORDS)     

马铃薯品种抗软腐病测定条件的研究

用全薯块吸头刺伤接种法测定时,较理想的测定条件为:每接种点接种0.1ml浓度为10~2～10~5CFU/ml菌悬液,随后在21±5℃～31±5℃(依所用菌株而定)下保持2～3天,以接种点腐烂斑的最大直径作记载标准。用新鲜薯片法测定时,除接种浓度为10~2～10~3CFU/ml,保持时间为1～2天及以侵染限值作评价标准外,其余条件和全薯块吸头刺伤接种法相同。当用皮孔浸渍法接种时,薯块在10_6CFU/ml菌悬液中浸5分钟,然后使薯块表面保持连续水膜,3～5天后测量薯块表面腐烂面积。上述3种方法都必须有20个以上重复,并使用大小一致、无伤无病的成熟薯块。     

不同地区黑蚱蝉的形态结构与鸣声比较研究

黑蚱蝉的鸣声和其发音器,具有基本的构造和特性,即种性.但也有一定的地理差异,鸣声有单音色,复合声,主峰频率高低等变化,这和形态的大小,发音器及其辅助发音器的变化相一致.黑蚱蝉的召唤声和求偶声是鸣声分类较理想的类型,而群鸣声不能用于种下分类.     

Destruction of Trichinella spiralis spiralis during the preparation of the "dry cured" pork products proscuitto, proscuittini and Genoa salami

Genoa salami, proscuittini and proscuitto were prepared from pork carcasses that were heavily infected experimentally with Trichinella spiralis spiralis. Genoa salami was prepared with salt concentrations of 2.0%, 2.75% and 3.3%. Proscuitto was prepared by two procedures approved by Agriculture Canada. At various times postpreparation, samples of the various cured products were taken and examined by pepsin digestion and rat bioassay for the presence of viable trichinae. Water activity and pH of the cured meat were also determined. Curing of the various products was shown to destroy the Trichinella larvae. Pepsin digestion revealed that larvae progressively became loosely coiled, uncoiled and more subject to digestion (ghost larvae) during the curing process. Rat bioassay revealed the presence of viable trichinae in the proscuitto prepared using a sodium chloride salt mixture at day 34 but not at day 48 postpreparation. All other bioassays carried out on Genoa salami between 13 and 42 days postpreparation, on proscuittini between days 27 and 69 and on proscuitto between days 34 and 69 were negative for viable trichinae. Under the conditions of this study, preparing Genoa salami with salt concentrations as low as 2% did not appear to affect the destruction of Trichinella larvae.