Predatory activity of the nematophagous fungus <Emphasis Type="Italic">Duddingtonia flagrans</Emphasis> on horse cyathostomin infective larvae

This work was performed to determine the predatory capacity in vitro of the nematophagous fungus Duddingtonia flagrans (isolate AC001) on cyathostomin infective larvae of horse (L₃). The experimental assay was carried out on plates with 2% water-agar (2% WA). In the treated group, each plate contained 1.000 L₃ and 1.000 conidia of the fungus. The control group without fungus only contained 1.000 L₃ in the plates. Ten random fields (4 mm diameter) were examined per plate of treated and control groups, every 24 h for seven days under an optical microscope (10× and 40× objective lens) for non-predated L₃ counts. After 7 days, the non-predated L₃ were recovered from the Petri dishes using the Baermann method. The interaction there was a significant reduction (p < 0.01) of 93.64% in the cyathostomin L₃ recovered. The results showed that the D. flagrans is a potential candidate to the biological control of horse cyathostomin L₃.     

Predatory activity of Pochonia chlamydosporia fungus on Toxocara (syn. Neoascaris) vitulorum eggs

Toxocara (Neoascaris) vitulorum is a gastrointestinal nematode parasite of young ruminants, responsible for high mortality rates in parasitized cattle and buffalo calves. The objective of this work was to compare the predatory capacity under laboratory conditions of four fungal isolates of the nematophagous fungus Pochonia chlamydosporia (VC1, VC4, VC5 and VC12) on T. vitulorum eggs in 2% water-agar (2% WA). T. vitulorum eggs were plated on 2% WA Petri dishes which contained cultured fungal isolates and control plates without fungi. After 10 and 15 days one hundred eggs were removed and classified according to the following parameters: type 1, biochemical and physiological effect without morphological damage to the eggshell, type 2, lytic effect with morphological alteration of the eggshell and embryo and type 3, lytic effect with morphological alteration of eggshell and embryo in addition to hyphal penetration and internal egg colonization. The fungal isolates were effective in the destruction of T. vitulorum eggs presenting the type 3 effect at 10 and 15 days after contact with the fungus. No nematophagous fungi were observed in the control group during the experiment. There was no variation in the predatory capacity of the fungal isolates (P?>?0.01) at the intervals of 10 and 15 days. These results indicate that P. chlamydosporia (VC1, VC4, VC5 and VC12) negatively influenced the development of T. vitulorum eggs and can be considered a potential candidate for the biological control of nematodes.     

Understanding fishing‐induced extinctions in the Amazon

相似文献   

Reverse transcription-duplex-polymerase chain reaction for simultaneous detection of Citrus tristeza virus and ‘Candidatus Liberibacter’ from citrus plants

Journal of Plant Diseases and Protection - Tristeza and Huanglongbing are the most important diseases of citrus caused by Citrus tristeza virus (CTV) and ‘Candidatus Liberibacter’...     

Effect of dietary carbohydrate to lipid ratios on growth,digestive enzyme and blood metabolites of juvenile Brazilian sardines,Sardinella brasiliensis (Steindachner, 1879)

The limited availability of live bait for capturing skipjack tuna, Katsuwonus pelamis, is a bottleneck to increasing tuna production in many parts of the world. Therefore, a nutrition trial was performed to contribute to the production of the Brazilian sardine, Sardinella brasiliensis, for use as live bait. This study determined the best dietary carbohydrate to lipid ratio (CHO:L) for juvenile Brazilian sardines based on growth performance, feed utilisation, body composition, blood metabolites and digestive enzyme activity. Six isoenergetic and isonitrogenous diets were formulated with increased CHO:L ratios (2.05, 3.41, 4.15, 5.11, 5.80 and 6.72). Each diet was randomly assigned to triplicate groups of 100 fish with mean initial body weight of 2.97 ± 0.51 g, which were fed four times a day to apparent satiation. Survival was not affected by differences in diet, however, a low CHO:L ratio stimulated growth. Juveniles fed with a rich‐carbohydrate diet inhibit feed intake and protein intake. Body lipid increased as dietary lipid increased and was inversely correlated to body moisture. The diets did not affect the juvenile's blood metabolites. Alkaline and acid protease activities were not significantly different, but lipase and amylase responded positively to the dietary lipids and carbohydrates. Using segmented regression, the optimum CHO:L ratio for maximum weight gain of juvenile Brazilian sardines was estimated to be 3.41, which contain approximately 300 g kg^?1 carbohydrate and 88 g kg^?1 lipid.     

Endogenous and exogenous polyamines in the organogenesis in Curcuma longa L.

The present work evaluated the development of different Curcuma longa L. explants (leaves basis, root tips and ancillary buds from rhizome) stimulated by exogenous polyamines, combined with naphtalen-acetic acid (NAA) or with 6-benzyl-aminopurine (BAP), to produce callus and its subsequent differentiation. The explants, isolated from field plants, were previously subjected to a basic cleaning method and were inoculated onto Murashige and Skoog culture medium (MS) [Murashige, T.S., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum 15, 473–497] supplemented with NAA (2.0 mg L⁻¹). Buds were subjected to different treatments, with or without 5.0 and 10.0 mmol L⁻¹ exogenous polyamines (mixture of putrescine:spermine:spermidine, 1:1:1) combined with NAA. The calluses obtained were transferred into the same medium, supplemented with the mixture of polyamines combined with BAP, in order to induce plant differentiation. For C. longa, buds were the most efficient explants for callus induction (p < 0.05). The application of exogenous polyamines (5.0 and 10.0 mmol L⁻¹) produced the most developed callus, with numerous roots. The medium supplemented with 10 mmol L⁻¹ polyamine mixture, combined with BAP, induced good regeneration, producing vigorous plants and excellent shoot formation.Polyamines addition promoted the formation of callus, roots and leaves, representing an important factor in the determination of indirect organogenesis in C. longa L., and putrescine content may be considered a valuable marker of the differentiation process in this specie, as well as the enzyme peroxidase.     

Effects of blood orange juice intake on antioxidant bioavailability and on different markers related to oxidative stress

Orange juice is a source of antioxidants that might afford in vivo protection from oxidative stress. To test this hypothesis, we carried out a human intervention study with blood orange juice containing high amounts of vitamin C, anthocyanins, and carotenoids. Sixteen healthy female volunteers were enrolled in a crossover study and were given 600 mL/day of blood orange juice or a diet without juice for 21 days. Before and after each intervention period, plasma vitamin C, cyanidin-3-glucoside, and carotenoids were quantified. Furthermore, plasma antioxidant capacity, malondialdehyde concentration in plasma, 11-dehydrotromboxane B(2) urinary excretion, and lymphocyte DNA damage were evaluated as biomarkers of oxidative stress. Blood orange juice consumption determined a significant increase in plasma vitamin C, cyanidin-3-glucoside, beta-cryptoxanthin, and zeaxanthin. Also, lymphocyte DNA resistance to oxidative stress was improved whereas no effect was observed on the other markers that we analyzed. In turn, these results suggest that blood orange juice is a bioavailable source of antioxidants, which might moderately improve the antioxidant defense system; however, the long-term effects of its consumption are to be further investigated.     

Role of different factors affecting the formation of 5-hydroxymethyl-2-furancarboxaldehyde in heated grape must

The influence of concentration and water activity (a(w)) on the formation of 5-hydroxymethyl-2-furancarboxaldehyde (HMF) in thermally treated grape must was evaluated. Must was cryoconcentrated and then heated to study the pure effect of sugar concentration. Moreover, NaCl was added to the must to lower a(w), maintaining the same sugar concentration, with the purpose of evaluating the pure effect of a(w). Finally, the influence of minimal pH changes on the formation of HMF was evaluated by means of a model solution. The results showed that a(w) and sugar concentration are both determinant in the formation of HMF in grape must. Sugar concentration influences the reaction by supplying substrates; low a(w) enhances the formation of HMF by changing the equilibrium in the dehydration step of the reaction.     

Hemangiosarcoma in 11 young horses

Hemangiosarcoma is a rare neoplasm of horses and hemangiosarcoma in young horses might behave differently than in mature horses. The purpose of this study was to identify the characteristics of hemangiosarcoma occurring in horses < or = 3 years of age. Medical records from 1982 to 2004 were searched for horses < or = 3 years of age with a histopathologic diagnosis of hemangiosarcoma. Eleven records were identified. Thoroughbred and Thoroughbred crosses predominated. Age ranged from 9 days to 3 years. All horses presented with cutaneous or leg swellings or joint effusion. Physical examination findings included tachycardia, fever, and depression. Laboratory abnormalities included anemia (5/11), hyperfibrinogenemia (4/11), hypofibrinogenemia (3/11), thrombocytopenia (2/11), and neutrophilic leukocytosis (1/11). Ultrasonographic and radiographic evaluation was not diagnostic in any case. Antemortem histopathologic diagnosis was obtained in 10 cases. Six of 11 horses were euthanized. Surgical resection was performed in 5 horses, 2 of which were later euthanized. Diagnosis was confirmed histologically at postmortem examination in all euthanized horses. Two cases resolved spontaneously. Early histopathologic diagnosis may allow cure if the mass is localized and amenable to surgical resection. In cases where the horse is medically stable, and masses are not interfering with quality of life, a period of observation may be warranted.