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171.
172.
根系不同分隔方式下油菜和鹰嘴豆对小麦锰营养的影响 总被引:1,自引:0,他引:1
通过根系分隔盆栽试验,研究了油菜和鹰嘴豆对小麦生长及锰营养的影响.结果表明:与油菜或鹰嘴豆混作能显著改善小麦生长及锰营养,其地上部干质量和吸收锰量均以根系不分隔处理显著高于根系完全分隔处理,但油菜和鹰嘴豆的生长受小麦抑制,表现为不分隔处理地上部干质量及吸锰量显著低于完全分隔.不施锰小麦/油菜和小麦/鹰嘴豆混作体系中,根系不分隔处理小麦根区土壤DTPA-Mn含量显著高于其他分隔方式,且油菜或鹰嘴豆地上部植株锰含量显著高于相应的混作小麦,在锰胁迫条件下,油菜和鹰嘴豆根系活化的土壤锰可被小麦吸收利用.因此与油菜或鹰嘴豆混作是改善小麦锰营养的有效途径之一. 相似文献
173.
基于多变异分组遗传算法的多机协同作业静态任务分配 总被引:2,自引:0,他引:2
为解决农业机械(简称农机)多机协同作业前的任务分配问题,提出了基于多变异分组遗传算法的同种农机多机协同作业静态任务分配方法。首先,根据农机合作社实际作业模式,基于农机性能参数和任务参数,综合考虑机群的作业时间、作业油耗和路程代价等因素,建立了多机协同代价函数;根据多机协同作业特点,构建了多变异分组遗传算法,设计了两段式编码、分组交叉算子和多种变异算子,并建立了农机多机协同作业静态任务分配模型;通过仿真试验对比分析了算法的性能,并采用不同的代价权重进行了任务分配仿真试验;最后,采用不同的权重对实际深松作业进行任务分配试验,对本文提出算法进行了验证。结果表明:在不同权重下,基于多变异分组遗传算法的多机协同静态任务分配的机群代价比实际作业代价降低了29.48%~55.00%,选取合理权重的静态任务分配具有较高的分配效率和分配性能,能够满足实际多机协同作业中任务分配的要求。 相似文献
174.
175.
针对丹参膜上倾斜移栽人工作业效率和质量较低、劳动强度较大、现有移栽机不适合丹参膜上倾斜移栽等问题,结合丹参大垄双行覆膜高效生产技术提出的膜上倾斜移栽农艺要求,设计一种基于变形椭圆齿轮-双变速曲柄五杆机构的鸭嘴式丹参膜上倾斜移栽机构。在移栽机构所要求的运动轨迹、栽植器倾斜姿态和设计要求的基础上,分析机构的工作原理并建立机构理论模型。依据数学模型运用Matlab开发出移栽机构人机交互可视化辅助程序,应用该辅助程序研究机构参数对栽植器倾斜角和栽植器端点轨迹的影响规律,通过人机交互的方式得到符合丹参膜上倾斜移栽机构农艺要求的参数组合。根据优化后的参数组合设计样机并进行虚拟仿真和样机田间试验,试验结果表明:变形椭圆齿轮-双变速曲柄五杆式丹参膜上倾斜移栽机构在满足丹参膜上倾斜移栽要求的同时能保证作业质量,移栽机构的立苗角度合格率为90. 7%、漏栽率为2. 7%、株距变异系数为5. 6%、栽植深度合格率为93. 7%。 相似文献
176.
驱导辅助充种气吸式精量排种器设计与试验 总被引:5,自引:0,他引:5
针对现有气吸式排种器充种环节工作压力要求高、高速作业时充种性能不佳,导致排种质量下降的问题,采用主动驱导种群、减少局部种间接触的方法,提高排种器高速条件下的充种率,并基于此设计了一种驱导辅助充种气吸式精量排种器。分析了排种器充种过程中种子的受力状态,阐述了排种盘导种槽辅助充种的工作原理,以及在充种过程各阶段提高种子充填率的设计思路;对导种槽外形曲线方程、斜面倾角进行了理论计算,确定了曲线的基本参数;利用气固耦合数值分析方法 CFD-DEM,以型孔处局部空隙率为指标,进行了单因素仿真试验,确定了导种槽曲线方程的基圆半径最优值。为了验证设计结果,在工作压力-5、-6 kPa条件下,分别与其他2种气吸式排种器进行速度单因素对比试验,试验结果表明,所设计的排种器在高速条件下的漏充率优于其他排种器。对所设计的排种器进行了前进速度、工作压力的双因素试验,通过多目标优化方法,确定了排种器的最佳工作参数。将所设计的排种器安装在气力式玉米免耕播种机上,在3个工作速度下进行了单因素田间试验,结果表明,当作业速度为9. 11 km/h时,粒距合格指数为95. 48%,高速条件下充种性能较为稳定。 相似文献
177.
AIM: To study the effect of fractalkine (CX3CL1, Fkn) on the expression and secretion of matrix metalloproteinase-2 (MMP-2) in cultured human monocyte line U937 cells. METHODS: The cultured U937 cells were incubated with recombinant human Fkn, the supernatant of human renal mesangial cells (HRMC) and Fkn neutralizing antibodies for 24 h. The mRNA expression of MMP-2 was analyzed by RT-PCR. The production of MMP-2 in the supernatant was analyzed by gelatin zymography. RESULTS: The level of MMP-2 mRNA and protein in the cells incubated with recombinant human Fkn decreased compared to control group. Similarly, the level of MMP-2 mRNA in the cells incubated with the supernatant of HRMC reduced compared to control group. However, the level of MMP-2 mRNA and protein in the cells incubated with the supernatant of HRMC adding Fkn neutralizing antibodies increased compared to that incubated with the supernatant of HRMC. CONCLUSION: Fkn inhibits the expression and secretion of MMP-2 in cultured U937 cells. HRMC might mediate the expression and secretion of MMP-2 in U937 cells through Fkn. 相似文献
178.
AIM:To investigate the expression of nestin, a kind of cytoskeletal protein in cultured murine podocytes and the role of nestin in the maintenance of the podocyte structure.METHODS:The immortalized murine podocytes were cultured. The expression of nestin was determined by immunofluorescence. In differentiated podocytes, the expression of nestin was knock-down by RNAi. The effect of nestin knock-down was examined by Western blotting and immunofluorescence. The projections longer than maximal length of the cell body in which the cells transfected with siRNA and control vector that contained nonhomologous oligo were counted, respectively. RESULTS:Nestin siRNA markedly reduced or abolished nestin expression. In cells transfected with nestin siRNA, the percentage of cells with processes was significantly lower than that in cells transfected with control vector (77.0%±6.3% vs 16.0%±4.6%, n=3, P<0.01). CONCLUSION:Nestin may play an important role in maintaining normal function of podocytes. 相似文献
179.
CAI Qing-qing LIN Tian-xin FANG Xin-lan YIN Xin-bao DONG Wen HUANG Li LIN Tong-yu 《园艺学报》2009,25(5):873-876
[ABSTRACT]AIM: To study the effect of 13-methyltetradecanoic acid (13-MTD), a saturated branched-chain fatty acid, on apoptotic induction in breast carcinoma cell line MCF-7 and its underlying mechanisms. METHODS: Breast carcinoma cell line MCF-7 and normal breast epithelial cells MaEC were treated with solvent or 13-MTD at concentration of 140 mg/L. Apoptosis was analyzed by flow cytometry. Phosphorylation of JNK, p38, FADD and Akt after treated with 13-MTD were detected by Western blotting. RESULTS: 13-MTD effectively induced apoptosis of breast carcinoma cell line MCF-7 and no influence to normal breast epithelial cells MaEC, which were confirmed by flow cytometry analysis, was observed. The results of Western blotting showed that obvious increase in p38 and JNK phosphorylation. No significant difference of FADD phosphorylation was observed. However, evidently decrease in Akt phosphorylation was found after treated with 13-MTD. CONCLUSION: 13-MTD was a new safe, effective chemotherapeutic drug. Its underlying mechanisms are through activating MAPK pathway and inhibiting Akt pathway to induce the cancer cells apoptosis. 相似文献
180.
JIANG Wen-qiang FANG Ming CHEN Chun-bo WANG Qiao-sheng YE Heng ZENG Hong-ke 《园艺学报》2009,25(4):755-758
AIM: To investigate the role of stress hyperglycemia on condition assessment and predicting prognosis in patients with sepsis. METHODS: The study included 44 patients with sepsis, divided into three groups according to their blood glucose profile within 24 h after admission: patients with stress hyperglycemia (group SH, n=15), diabetes mellitus type 2 (group DM, n=10), and normal glucose levels (group NG, n=19). CD4+/CD8+ ratio, Th1/Th2 ratio and HLA-DR% of the patients were measured within 24 h after admission by flow cytometry as assessment of their immune function. The sepsis-related organ failure assessment (SOFA) scores and acute physiology and chronic health evaluation II (APACHE II) scores of patients were recorded at the same time to analyze whether stress hyperglycemia affects the immune function and the 28 d mortality in patients with sepsis. RESULTS: A higher mortality rate of septic patients with stress hyperglycemia was observed compared to diabetic patients (53.3% vs 10.0%) and group NG (53.3% vs 21.1%). SOFA score and APACHE II score were higher in group SH than those in group DM and group NG while lower levels of human leucocyte antigen DR (HLA-DR) expression and CD4+/CD8+ ratio was found in group SH than those in group DM and group NG. No difference in the levels of Th1/Th2 among the three groups was observed. Non-survivors had higher levels of SOFA score, APACHEⅡ score, Th1/Th2 ratio and HLA-DR%. No difference was detected for CD4+/CD8+ ratio, mean glucose values and age. CONCLUSION: Stress hyperglycemia is associated with decreased immune function and an adverse clinical outcome in patients with sepsis. 相似文献