聚醚类抗生素——腐霉素发酵条件的研究

由吸水链霉菌 （Ｓｔｒｅｐｔｏｍ ｙｃｅｓｈｙｇｒｏｓｃｏｐｉｃｕｓ）ＨＳ－ ８６诱变获得的一株突变株Ｕ－５８,通过设计的多因子试验,确定了培养该菌株的最佳碳、氮源比,诸因子中影响最大的是淀粉,其次是蛋白胨、酵母粉。原材料对比试验中,淀粉以玉米淀粉最佳。锰离子对腐霉素产量的提高有显著的促进作用。在２．５ｔ发酵罐中通气培养９６～１２０ｈ,效价达到４ ０００μｇ／ｍ ｌ以上。     

乳胶凝集试验与血清中和试验检测猪伪狂犬病血清抗体的 …

应用血清中和试验（ＳＮＴ）和伪狂犬病乳胶凝集试验（ＬＡＴ）诊断试剂盒对两种伪狂犬病是性血清、伪狂犬病病毒（ＰＲＶ）高兔血甭及６０份被检猪血清进行了ＰＲＶ抗体效价测定和相关性分析,两种方法测得的抗体效价之间呈强相关性（ｒ＝０．９６）,且ＬＡＴ效价比ＳＮＴ一般高出一个滴度;能干为自３５个猪场的４１４份猪血清进行了ＰＲＶ抗体检测,并与ＳＮＴ检测结果进行了对比,结果在ＳＮＴ检测为阳笥的１７１份血清中,ＬＡ     

在生物经济中发展柞蚕产业

2009亚太国家生物经济会议《亚太生物经济项目册》中有五个蚕业项目,充分显示了我国蚕业在生物经济中的地位和发展潜力。本文引入生物经济的概念,介绍了我国柞蚕生物工程的概况,提出用工业化理念发展蚕业,要用现代信息产业改造传统蚕业,在生物经济领域突破发展柞蚕产业。     

猪带绦虫六钩蚴TSOL18基因疫苗表达载体的构建及其免疫原性研究

为研制新型猪囊尾蚴疫苗,将猪带绦虫六钩蚴阶段编码 TSOL18 的基因定向克隆于真核表达质粒 pVAX1, 经筛选、鉴定及 DNA序列分析正确后,将重组质粒 pVAX1/TSOL18 转染 BHK-21 细胞,通过 SDS-PAGE、Western blotting、免疫荧光试验等方法检测细胞中表达的 TSOL18 抗原。结果表明,重组真核质粒 pVAX1/TSOL18 可在 BHK-21 细胞中表达TSOL18 目的蛋白,表达蛋白能被猪囊尾蚴病阳性血清所识别。动物免疫试验表明,真核表达载体能有效地诱导机体产生细胞免疫和体液免疫应答,这为猪囊尾蚴病基因疫苗的研究奠定了良好基础。     

Study on the Change Rule of Egg Yolk Antibody in Laying Hens after First Immunization by Japanese Encephalitis Virus

The purpose of this experiment was to study the immunization rule of the egg yolk antibody affected by different vaccines,immunization dose and injection ways and further to discuss the optimal immunization procedures of the laying hens for the preparation of egg yolk antibody against swine Japanese encephalitis virus.180 brown laying hens without any vaccines were selected and divided into 18 groups randomly,each group of 10 hens.Groups 1,2 were the control groups,injected with the sterile saline;Groups 3 to 10 were injected with subcutaneous or intramuscular injection,and the vaccine was injected with 0.2,0.5,1.0 and 1.5 mL successively.Groups 11 to 18 were also adopted two kinds of injection,followed by the same dose of vaccine immunization.Six eggs of each experimental group were gathered before immune day and after 3,7,10,14,18,21 and 28 days,the egg yolk antibody was extracted and the titer was determined.As a result,the egg yolk antibody titers of groups 1 to 6,11 and 12 were all 0,and no significant immune response produced;The hens from 7 to 10 groups were injected with the inactivated vaccine.After 7 days,the average antibody titer reached the peak,and the duration of the antibody was 14 days.The hens from 13 to 18 groups were injected with the attenuated virus vaccine.After 14 days,the average antibody titer reached the highest value,and the duration of the antibody was 21 days.The egg yolk antibody titers were not significantly different in the two compared experiment groups with the same injection dose but with different injection ways (P>0.05).With the same injection way of each experiment group,and the difference was significant (P>0.05).Compared with some groups with the same injection and vaccine,the titer of yolk antibody was gradually increased with the increase of the immune dose,and the difference was significant (P<0.05).The results showed that,no matter intramuscular or subcutaneous injection,in order to produce a significant immune response to hens,the immune antigen dose was 1.0 mL inactivated vaccine or 0.5 mL attenuated vaccine at least.Compared with the attenuated and inactivated vaccine,inactivated vaccine stimulated the body to produce the antibody faster,but the maintenance time was shorter;The lower dose of attenuated vaccine could stimulate the body to produce antibodies,but the speed was slower,the maintenance time was longer.     

恩诺沙星混悬液在猪体内残留消除规律研究

采用高效液相色谱法研究恩诺沙星(口服)混悬液在猪体内各组织中的残留消除规律.恩诺沙星(口服)混悬液,按每头猪10 mg/kg体重的剂量灌服给药,连续使用3 d之后,宰杀猪,取组织.组织样品经磷酸盐缓冲液提取,C18固相萃取柱净化,过膜,用流动相0.05 mol/L磷酸溶液/三乙胺一乙腈(82+18)溶解,微孔过滤,进行...     

3种兔球虫18S rDNA部分序列测定与系统发育分析

采用单卵囊分离法从河北某兔场分离大型艾美耳球虫、黄艾美耳球虫及肠艾美耳球虫,接种无球虫兔后获得大量纯种卵囊,CTAB法提取孢子化卵囊基因组DNA。利用艾美耳属球虫18S rDNA保守引物,PCR扩增3种兔球虫18S rDNA片段,产物纯化后测序。将3种球虫18S rDNA测序结果与GenBank中发布的兔球虫18S rDNA序列用DNAStar软件进行比对。使用MEGA4.0软件对兔球虫18S rDNA进行同源性比较,并绘制遗传进化树。结果表明,大型艾美耳球虫扩增出大小为1 521bp的18S rDNA片段;黄艾美耳球虫及肠艾美耳球虫均扩增出大小为1 520bp的18S rDNA片段。序列比对结果显示,3种河北株兔球虫与GenBank中相应的3种兔球虫18S rD-NA(EF694016、EF694011、EF694012)相似性分别为99.6%、99.6%和100%。3种河北株兔球虫序列和GenBank中兔球虫18S rDNA序列(EF694007-EF694017)位于一个单系集群。     

优化高效液相色谱法测定棉籽粕中游离棉酚的含量

本试验旨在建立一种测定棉籽粕中游离棉酚含量的高效液相色谱(hPLC)法的优化方法.试验采用丙酮提取棉籽粕样品,经超声、离心、抽滤、旋转蒸发处理,用乙腈-0.2％磷酸(体积比为85∶15)溶液溶解;色谱条件为:色谱柱Agilent TC-C18(250mm×4.6mm,5μm),流动相为乙腈-0.2％磷酸(体积比为85∶15)溶液,流速1.0mL/min,紫外检测波长235nm,进样量20μL,柱温25℃.结果得出,游离棉酚的回归标准曲线为y=189347x+24969(r=0.99999),在游离棉酚含量为0.1224～78.336 μg/mL范围内线性良好,最低定量限为0.52mg/kg;棉籽粕样品36h内4℃稳定性的相对标准偏差在1.51％～3.43％;平均回收率在94.72％～99.49％,相对标准偏差在1.72％～3.13％.可知,本方法稳定性好,准确度、灵敏度和回收率高.     

仔犬主要感觉行为发育的观察

犬的感觉行为是犬最基本的行为构成,其发育是一个连续的过程.了解和掌握仔犬期(0-45日龄)主要感觉行为的发育特征,对提高仔犬感觉统合能力、行为发育、仔犬期的饲养管理水平和训练水平具有重要意义.