Effects of pig slurry application on soils and soil humic acids

The effect of three annually consecutive additions of pig slurry at two rates (90 and 150 m3 x ha(-1) x year(-1) on soils and soil humic acids (HAs) was investigated in a field experiment under semiarid conditions. Soils and pig slurries were analyzed by standard methods. The HAs were isolated from soils and pig slurry by a conventional procedure based on alkaline extraction, acidic precipitation to pH 1, purification by repeated alkaline dissolutions and acidic precipitations, water washing, dialysis, and final freeze-drying. The HAs obtained were analyzed for elemental (C, H, N, S, and O) and acidic functional group (carboxylic and phenolic) composition, and by UV-vis, FT-IR, fluorescence, and ESR spectroscopies. With respect to the control soil, the pig slurry amended soils had greater pH and electrical conductivity, slightly larger total N content, and smaller values of C/N ratio. A decrease of total organic C was observed only in soils amended for 2 and 3 years at the higher slurry rate. With respect to control soil HA, pig slurry HA was characterized by larger contents of S- and N-containing groups, smaller acidic functional group and organic free radical contents, a prevalent aliphatic character, extended molecular heterogeneity, and smaller aromatic polycondensation and humification degrees. Amendment with pig slurry HA determines a number of modifications in soil HAs, including increase of C, S, and COOH contents, C/N ratios, and aliphaticity and decrease of extraction yields and N, O, phenolic OH, and organic free radical contents. These effects are generally more evident after the first year of slurry application and tend to disappear with increasing number of treatments. Most probably, over the years the slightly humified slurry HA is mineralized through extended microbial oxidation, whereas only the most recalcitrant components, such as S-containing, phenolic, and aliphatic structures, are partially accumulated by incorporation into soil HA.     

Soil management for Alfisols in the semiarid tropics: erosion, enrichment ratios and runoff

Abstract. Continuous cultivation of soils of the semiarid tropics has led to significant land degradation. Soil erosion and nutrient loss caused by high runoff volumes have reduced crop yields and contributed to offsite damage. We compared a number of soil management practices (tillage, mulch and perennial/annual rotational based systems) for their potential to improve crop production and land resource protection in an Alfisol of the semiarid tropics of India. Runoff and soil erosion were monitored and surface soil and sediment were analysed for nitrogen and carbon to determine enrichment ratios. Amelioration of soils with organic additions (farmyard manure, rice straw) or rotating perennial pasture with annual crops increased soil carbon and nitrogen contents and reduced runoff, soil erosion and nutrient loss. Soil erosion totalled less than 7 t ha^–1, but enrichment ratios were often greater than 2 resulting in up to 27 kg N ha^–1 and 178 kg C ha^–1 being lost in sediment. Up to an extra 250 mm of water per year infiltrated the soil with organic additions and was available for crop water use or percolation to groundwater. The results show that there are good opportunities for reducing degradation and increasing productivity on farms.     

Habitat fragmentation in an urban environment: large and small fragments support different arthropod assemblages

We investigated the effects of fragmentation due to urbanisation on the species composition and functional roles of ants, beetles, spiders, flies and wasps. The study was conducted in 21 fragments of heath and woodland in south-eastern Australia classed as either ‘small’ (? 4 km²) or ‘large’ (? 80 km²). Arthropods were pitfall-trapped and identified to family or genus and morphospecies and microhabitat characteristics were recorded. Large fragments did not support more species per unit area than small fragments for most arthropods, although there were more species of ants per sampling unit in small than large woodland fragments, mainly due to a higher frequency of generalist species in smaller fragments. Large and small habitat fragments contained different assemblages of spiders, wasps and ants, indicating that predators and parasitoids are affected more strongly than other trophic groups. Arthropod assemblages within larger fragments where grids were furthest apart were less similar than those within smaller fragments where grids were closer together in woodland, but not in heath. The responses of arthropods to fragmentation suggest that, in addition to effects of reduced area and proximity to the urban matrix, changes in fire regimes and the degradation of habitats resulting from urbanisation, may have a role in altering arthropod assemblages, particularly affecting those species belonging to higher trophic levels. Management goals for urban remnants should identify mechanisms for controlling fire and anthropogenic disturbance such that they closely resemble the levels of these factors in larger fragments.     

孔隙结构图像分析中不同试验因素对分析结果的影响

研究了不同试验因素 (包括图像分辨率、土壤切片定向性、分析区域大小等 )对孔隙结构图像分析结果的影响以及试验误差 ,并简要介绍了用于分析土壤孔隙结构的土壤切片及数字图像的制备技术。结果表明 :不同试验因素对分析结果均会产生一定的影响 ,试验误差约在 1 0 %。这表明同一研究中应保持试验条件的相对一致以保证分析结果的可靠性和可比性     

Crop responses to applied soil compaction and to compaction repair treatments

Crop responses to annual compaction treatments (applied to whole plots) and management treatments to ameliorate compacted soil were determined in a field experiment on a Vertisol. Initially, all treatments except a control were compacted with a 10 Mg axle load on wet soil (26% gravimetric water content compared with a plastic limit of 22%). Annually applied axle loads of 10 and 6 Mg on wet soil (25–32% soil water) tended to reduce seedling emergence, grain yield (wheat, sorghum and maize), soil water storage and crop water use efficiency (WUE). Annual applications of an axle load of 6 Mg on dry soil (<22% soil water) had little effect on crop performance. Mean reductions in the yield of five crops (three wheat, one sorghum and one maize) in comparison with the uncompacted control were 23% or 0.79 Mg ha⁻¹ (10 Mg on wet soil), 13% or 0.44 Mg ha⁻¹ (6 Mg on wet soil) and 1% or 0.03 Mg ha⁻¹ (6 Mg on dry soil). Maize grown in the fifth year of treatment application was most affected by compaction of wet soil, its WUE being reduced from 14.3 to 9.7 kg ha⁻¹ mm⁻¹ in response to an axle load of 10 Mg. Reduced WUE was associated with delayed soil water extraction at depth. A 3-year pasture ley was the most successful amelioration treatment. A wheat and a maize crop grown after the ley outyielded the control by 0.33 and 0.90 Mg ha⁻¹, respectively. So the pasture not only ameliorated the initial compaction damage, with respect to crop performance, but resulted in improvements in two subsequent crops.     

Evidence of the glycation and denaturation of LTP1 during the malting and brewing process

The influence of malting and brewing processes on the chemical and structural modifications occurring on LTP1 was investigated by mass spectrometry and circular dichroism. Proteins were first purified from malt, and samples were collected at various steps of beer processing performed on two barley cultivars. The levels of LTP1 found in malt were not significantly different from the amounts in barley seed. However, in malt, both LTP1b, a post-translational form of LTP1, and a third isoform named LTP1c were isolated. Moreover, both of these proteins were found to be heterogeneously glycated but still exhibited an alpha-helix structure. Both glycated LTP1 and LTP1b were recovered during mashing. It was also shown that glycated LTP1 was unfolded during heat treatment of wort boiling, which is in agreement with the denatured form previously isolated from beer.     

Microbial activity affected by lime in a long-term no-till soil

Under conventional farming practices, lime is usually applied on the soil surface and then incorporated into the soil to correct soil acidity. In no-till (NT) systems, where lime is surface applied or only incorporated into the soil to very shallow depth, lime will likely not move to where it is required within reasonable time. Consequently, lime may have to be incorporated into the soil by mechanical means. The objective of this laboratory study was to characterize the effect of lime, incorporated to different depths, on chemical and biological soil properties in a long-term NT soil. Soil samples taken from the 0–5, 0–10, and 0–20 cm depths were analyzed in incubation studies for soil pH, nitrate, CO₂ respiration, and microbial biomass-C (MBC). Lime (CaCO₃) was applied at rates equivalent to 0, 4.4, 8.8, and 17.6 Mg ha⁻¹. Application of lime to both 0–10 and 0–20 cm depths increased soil pH from about 4.9 by 1, 1.7, and 2.8 units for the low, medium, and high liming rates, respectively. Soil nitrate increased over time and in proportion to liming rate, suggesting that conditions were favorable for N-mineralization and nitrification. Greater respiration rates and greater MBC found in lime-treated than in non-limed soils were attributed to higher soil pH. Faster turnover rates and increased mineralization of organic matter were found in lime-treated than in non-limed soils. These studies show that below-surface lime placement is effective for correcting soil acidity under NT and that microbial activity and nitrification can be enhanced.     

Effects of tillage systems on soil characteristics, glomalin and mycorrhizal propagules in a Chilean Ultisol

Tillage affects the soil physical and chemical environment in which soil microorganisms live, thereby affecting their number, diversity and activity. However, soil disturbance generally has the greatest impact on biological properties, including both free and symbiotic fungal populations. Interest in more ecologically sustainable agricultural systems is rising with increasing recognition that agricultural intensification can adversely affect environmental quality. This paper discusses the effect of tillage system on some soil characteristics, such as pH, C, N and S levels, total and Olsen-P contents including some P forms associated with organic matter, glomalin contents and arbuscular mycorrhizae (AM) parameters, such as root colonization, spore number and total and active hyphal length. Measurements were in the sixth year of an on-going tillage-rotation experiment conducted on an Ultisol under no-till (NT), reduced tillage (RT) and conventional tillage with stubble mixed into the soil (CTS) or stubble burnt (CTB). Soil was sampled at two dates; after wheat (Triticum aestivum) harvest (autumn) and 6 months after subsequent grassland seeding (spring). Higher C, N, S, total P and fulvic acid-P concentrations and pH occurred under NT and RT than under CTS and CTB after wheat harvest. However, results at the second sampling were not consistent. AM spore number and active hyphal length were highest under NT having the greatest incidence on AM root colonization and P concentration in shoots of the pasture. Glomalin concentration was higher under NT and RT than under CTS and CTB but no differences in calculated glomalin to total C (ca. 5%) were found. It is concluded that a less disruptive effect of NT influences positively all soil characteristics and also increases P acquisition by the following crop in the rotation system.     

Characterization of odor-active volatiles in apples: influence of cultivars and maturity stage

The aroma and texture of three different apple cultivars, harvested at three maturity stages, were analyzed by sensory and instrumental analysis. The emphasis was on the identification of the most potent odorant volatiles, and the challenge was to separate the few most important flavor compounds, which may be trace chemicals, from the vast number of nonodorant compounds present in apple aroma extracts. Thirty-six odorant compounds were detected, 24 of which were common to all extracts. A significant correlation coefficient was found between the aroma intensity scores and overall quantity of the odorant volatiles, which shows that the development of sensory aroma is similar to that of odorant volatiles. This study also showed that the parameters measured by penetrometry and compression were highly correlated with sensory textural attributes. The determination of the optimal maturity stage for different apple cultivars by the usual parameters, such as color, diameter, total soluble solids, and titrable acidity, may not be sufficient to determine the optimal sensory quality for consumers. Moreover, the sensory quality of fruits changes during maturation in a different way from one cultivar to another, and this should be taken into account.     

Comparative evaluation of the emulsifying properties of phosphatidylcholine after enzymatic acyl modification

The ability of enzymatically synthesized structured phosphatidylcholine (PC) containing caprylic acid to form and stabilize oil-in-water emulsions prepared with different triglycerides [medium chain triglycerides (MCT), soybean oil, and enzymatically synthesized structured lipids] was examined and compared with natural soybean PC and deoiled lecithin. Emulsions were prepared with varying oil and emulsifier concentrations. The particle size distribution, creaming stability, and viscosity were measured for the evaluation of the emulsifying properties. With an increase in the oil concentration, there was an increase in particle size, viscosity, and creaming layer. With an increase in the phospholipid (PL) concentration, there was usually a decrease in particle size and an increase in viscosity, where the emulsion stability was increased. General emulsions prepared with structured lipids resulted in smaller particle sizes as compared to MCT and soybean oil. Deoiled lecithin was able to increase the viscosity more significantly and give smaller particle sizes as compared to the other emulsifiers, thus producing more stable emulsions. However, in certain cases, structured PC was superior to deoiled lecithin and soybean PC. This observation was made for emulsions prepared with soybean oil or structured lipid at an oil/water ratio of 10:90. At an oil/water ratio of 30:70, the deoiled lecithin performed better as compared to the other PLs with all oil types. However, structured PC produced more stable emulsions as compared to natural soybean PC in MCT and soybean oil.