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231.
Diversity Among Isolates of Bean pod mottle virus 总被引:1,自引:0,他引:1
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233.
In the UK, welfare organisations care for several thousand dogs each year. The successful rehoming of these dogs is a difficult process resulting in some of them being returned to rehoming centres. There have been very few studies examining the underlying mechanisms in the UK. Therefore, this study was conducted to determine those factors which affect the success of rehoming dogs.A prospective cohort study was conducted using a sample of 5750 dogs rehomed by Dogs Trust, a UK based dog welfare charity, during 2005. Dogs were followed up for a period of 6 months after adoption to determine if these dogs were still in their placement home. There was a 78% response rate to the follow-up postal questionnaires sent to the new owners, giving information on 4500 owners. Fourteen percent of adoptions failed. The results showed that behavioural problems are an important factor in the success of adoption such that if dogs had shown aggression towards people and the owners had not sought advice, they had 11.1 times the odds (95% CI: 6.6, 18.8) of being returned compared to those dogs without behavioural problems. Attending training classes significantly decreased the chance that the adoption would be unsuccessful (OR 0.3, 95% CI: 0.2, 0.4). It was shown that those owners who found that the effort and work involved in looking after their dog to be more than they had expected, had 9.9 (95% CI: 4.1, 24.6) times the odds of returning their dog than those who found the effort required to be less than they had expected.The results of this study show that there are many factors involved in a successful adoption and it is important that the new owners are informed of what to expect are encouraged to attend training classes and are prepared to work at any behavioural problems that their dog may have. 相似文献
234.
AIM: To identify some production and reproductive effects of calving induction in seasonally calving herds. METHODS: Forty seasonally calving herds entered on the DairyMAN management information system and whose milk yield and reproductive data were recorded, including pregnancy diagnosis results, were included in the study. Cows with an induced parturition were compared with normally calving contemporaries that had the same lactation number and calved at the same time. RESULTS: Milk yield was 1.2 +/- 0.2 litres/cow/day less over the entire lactation for cows induced to calve, with the greatest difference being 2.5 +/- 0.4 litres/cow/day measured in early lactation. The effects on milkfat and protein yield were similar, with 0.04 +/- 0.01 kg/cow/day less milkfat (p < 0.0001) and 0.03 +/- 0.01 kg/cow/day less protein (p < 0.0001). A higher milkfat percentage (+ 0.09 +/- 0.04 %) (p < 0.0001) and protein percentage (+ 0.10 +/- 0.02%) (p < 0.0001) for cows that were induced to calve reduced the effect of a lower milk yield on milkfat and protein production. The first service conception rate for cows induced to calve was 54.4 +/- 3.3%, which was significantly less (p = 0.03) than for cows that calved normally (59.5 +/- 3.3%). Cows induced to calve had a pregnancy rate at the end of mating of 91.4 +/- 2.1%, which was also significantly less (p <0.0001) than for cows that calved normally (93.6 +/- 1.7%). The 21-day submission rates were not significantly different between groups. CONCLUSIONS: This study indicates that, under some New Zealand management conditions, the induction of calving is associated with reduced daily milk yield and inferior reproductive performance. 相似文献
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Current knowledge on the epidemiology of bovine tuberculosis in New Zealand is reviewed, with emphasis on recent findings. It would appear that the epidemiology of the disease is determined by the behaviour of both wildlife and domestic stock, and environmental influences on the development of the infectious state in wildlife. The central cause of persistent disease nationally remains the possum, although other species may play a subsidiary role locally. Current understanding provides much improved prospects for control, which will be based on implementing individual farm control strategies to complement current regional control, with additional later prospects of vaccination of wildlife and possibly reproductive control of possums. 相似文献
237.
Evaluation of three serological assays for the diagnosis of Mycobacterium bovis infection in brushtail possums 总被引:1,自引:0,他引:1
Buddle BM Nolan A McCarthy AR Heslop J Aldwell FE Jackson R Pfeiffer DU 《New Zealand veterinary journal》1995,43(3):91-95
Three serological tests for the diagnosis of Mycobacterium bovis infection were evaluated on 29 possums (Trichosurus vulpecula) with tuberculosis and on 100 possums from a tuberculosis-free area. An indirect ELISA using M. bovis culture filtrate as the antigen had a sensitivity of 45% and a specificity of 96%, while an indirect ELISA using a M. bovis specific antigen (MPB70) had a sensitivity of 21% and a specificity of 98%. A blocking ELISA which utilised a monoclonal antibody against MPB70 had a sensitivity of 28% and a specificity of 99%. Combination of the test results of the three ELISAs resulted in an increase in sensitivity to 51% and a decrease in specificity to 93%. A previous study has shown that possums experimentally infected with M. bovis produced cellular responses to M. bovis antigens relatively early in the infection, but these responses decreased in the terminal stages of the disease. In contrast, analysis of serological responses in the current study from sequentially collected sera of possums experimentally and naturally infected with M. bovis showed that antibody was first detected late in the disease. 相似文献
238.
Principles and practical application of the receiver-operating characteristic analysis for diagnostic tests 总被引:1,自引:0,他引:1
We review the principles and practical application of receiver-operating characteristic (ROC) analysis for diagnostic tests. ROC analysis can be used for diagnostic tests with outcomes measured on ordinal, interval or ratio scales. The dependence of the diagnostic sensitivity and specificity on the selected cut-off value must be considered for a full test evaluation and for test comparison. All possible combinations of sensitivity and specificity that can be achieved by changing the test's cut-off value can be summarised using a single parameter; the area under the ROC curve. The ROC technique can also be used to optimise cut-off values with regard to a given prevalence in the target population and cost ratio of false-positive and false-negative results. However, plots of optimisation parameters against the selected cut-off value provide a more-direct method for cut-off selection. Candidates for such optimisation parameters are linear combinations of sensitivity and specificity (with weights selected to reflect the decision-making situation), odds ratio, chance-corrected measures of association (e. g. kappa) and likelihood ratios. We discuss some recent developments in ROC analysis, including meta-analysis of diagnostic tests, correlated ROC curves (paired-sample design) and chance- and prevalence-corrected ROC curves. 相似文献
239.
This investigation examined primarily epidermal specializations of the adult horse tongue by light, scanning and transmission electron microscopy. Samples were collected from seven regions of the normal tongue of various breeds of horse. The filiform papillae, present on the dorsal and lateral aspects but not the ventral aspect of the tongue, were short, slender and finger-like structures with variable-shaped terminae. The epidermal thickness and height of dermal ridges were reduced on fungiform and vallate papillae, but tissue architecture and keratinocyte ultrastructure of most of the lingual epidermis corresponded to the common mammalian epidermal paradigm. One unique finding was the highly localized clustering of epidermal cells with exceptionally high content of PAS-negative trichohyalin cytoplasmic granules at a location atop the dermal ridges and beneath the base of filiform papillae. These granular cells were immediately subjacent to clusters of clear, non-granulated epidermal cells. It is believed that this integumentary specialization may enhance the structural strength at this localized site of the tissue architecture, in relationship to the mechanical papillae. 相似文献
240.
Thirty cohort pigs were followed from birth to slaughter to study epidemiological patterns of porcine pleuropneumonia caused by Actinobacillus pleuropneumoniae. The study was conducted within a larger 380-animal study of vaccination against Mycoplasma hyopneumoniae and A. pleuropneumoniae in a 340-sow farrow-to-finish piggery with 4-month weaning, operating a continuous system of intensive production in the North Island of New Zealand. The cohort pigs were randomly allocated into two equal groups: vaccinated and control. Pigs in the first group were vaccinated at 2 and 4 weeks of age with both M. hyopneumoniae vaccine and A. pleuropneumoniae vaccine at separate vaccination sites. A series of nasal swabs was taken at 4, 8, 10, 11, 12, 14, 16 and 18 weeks of age. Each swab was streaked onto the surface of a selective medium on the farm and the plates were immediately transported to a laboratory and incubated at 37 degrees C for 5 days. After the trial, pigs were slaughtered at an average of 132 days of age, lungs were examined and samples taken for bacteriological culture and isolation. Thirty-five out of 256 samples produced haemolytic colonies which were Gram-negative, V-factor-dependent and positive to the CAMP test. A. pleuropneumoniae was first isolated at 4 weeks of age from one vaccinated pig. This finding suggests that piglets became infected in the farrowing pen and the source of infection might be a carrier sow. The interval-specific cumulative incidence of A. pleuropneumoniae infection reached a maximum of 54% and 40% at 11 weeks of age in the vaccinated and control groups, respectively. Infection status of the litter is considered to be a factor influencing morbidity in infected herds during weaner and grower periods. Our results suggest that simultaneous vaccination with M. hyopneumoniae and A. pleuropneumoniae vaccines at 2 and 4 weeks of age might lessen the prevalence but cannot absolutely prevent A. pleuropneumoniae infection during the weaner or grower-finisher periods. 相似文献