Analytical and sensorial characterization of the aroma of wines produced with sour rotten grapes using GC-O and GC-MS: identification of key aroma compounds

In the present work, the aroma profiles of wines elaborated from sound and sour rot-infected grapes as raw material have been studied by sensory analysis, gas chromatography-olfactometry (GC-O), and gas chromatography-mass spectrometry (GC-MS), with the aim of determining the odor volatiles most likely associated with this disease. The effect of sour rot was tested in monovarietal wines produced with the Portuguese red grape variety Trincadeira and in blends of Cabernet Sauvignon and sour rotten Trincadeira grapes. Wines produced from damaged berries exhibited clear honey-like notes not evoked by healthy samples. Ethyl phenylacetate (EPhA) and phenylacetic acid (PAA), both exhibiting sweet honey-like aromas, emerged as key aroma compounds of sour rotten wines. Their levels were 1 order of magnitude above those found in controls and reached 304 and 1668 μg L(-1) of EPhA and PAA, respectively, well above the corresponding odor thresholds. Levels of γ-nonalactone also increased by a factor 3 in sour rot samples. Results also suggest that sour rot exerts a great effect on the secondary metabolism of yeast, decreasing the levels of volatiles related to fatty acids and amino acid synthesis. The highest levels of γ-decalactone of up to 405 μg L(-1) were also found in all of the samples, suggesting that this could be a relevant aroma compound in Trincadeira wine aroma.     

Horizontal matter fluxes and leaching losses in urban and peri‐urban agriculture of Kabul,Afghanistan

Little is known about nutrient fluxes and nutrient‐use efficiencies in urban and peri‐urban agriculture (UPA) of rapidly expanding cities in developing countries. Therefore, horizontal flows of carbon (C), nitrogen (N), phosphorus (P), and potassium (K) as well as leaching losses of mineral N and P were measured over 2 years in three representative agricultural production systems of Kabul. These comprised 21 gardens and 18 fields dedicated to vegetable farming, cereal farming, and table‐grape production (vineyards). Across sites (fields and gardens) biennial inputs averaged 375 kg N ha^–1, 155 kg P ha^–1, 145 kg K ha^–1, and 15 kg C ha^–1 while with harvests 305 kg N ha^–1, 40 kg P ha^–1, 330 kg K ha^–1, and 7 kg C ha^–1 were removed. In vegetable gardens, biennial net balances were 80 kg N ha^–1, 75 kg P ha^–1, –205 kg K ha^–1, and 4 kg C ha^–1, whereas in cereal farming biennial horizontal balances amounted to –155 kg N ha^–1, 20 kg P ha^–1, –355 kg K ha^–1, and 5 kg C ha^–1. In vineyards, corresponding values were 295 kg N ha^–1, 235 kg P ha^–1, 5 kg K ha^–1, and 3 kg C ha^–1. Annual leaching losses in two selected vegetable gardens varied from 70 to 205 kg N ha^–1 and from 5 to 10 kg P ha^–1. Night soil and irrigation water were the major sources among the applied nutrient inputs in all studied farming systems, contributing on average 12% and 25% to total N, 22% and 12% to total P, 41% and 53% to total K, and 79% and 10% to total C, respectively. The results suggest that soils in extensive cereal fields are at risk of N and K depletion and in vegetable gardens of K depletion, while vineyards may be oversupplied with nutrients possibly contributing to groundwater contamination. This merits verification.     

Management of human health risk in the context of kitchen gardens polluted by lead and cadmium near a lead recycling company

Purpose

At the global scale, gardening activities are often performed in urban areas with a historical background of pollution. In this study, a participatory program was developed with citizens concerned by gardening activities near a 50-year-old regulated lead recycling company, with the aim of co-constructing the tools for the assessment and management of potential sanitary risks induced by historic pollution with persistent (eco) toxic metals: lead and cadmium.

Materials and methods

Soils and vegetables (lettuce, leek, celery, carrot, chard, pumpkin, and celeriac) samples were collected from four kitchen gardens neighboring a 50-year-old secondary lead smelter. Both total and in vitro human bioaccessible metal concentrations in the cultivated plants were measured in relation to soil characteristics.

Results and discussion

The results showed that the soils of these gardens were slightly contaminated by metals (Pb, 77 to 236 mg kg^?1; and Cd, 0.5 to 1 mg kg^?1) in comparison with the natural geologic background. However, significant pollution of vegetables can occur especially with lead (Pb up to 9.8 mg kg^?1 in lettuce) and certainly as a result of direct foliar transfer. The washing of plants before consumption is therefore recommended in the context of atmospheric fallout of ultrafine particles enriched with metals.

Conclusions

Metal bioaccessibility measure integrates the influence of metal type, plant type, and soil physico-chemical properties. Based on the results, it is proposed that human bioaccessible fraction of metals may also be currently taken into account as well as total metal quantities and bioaccumulation factors in risk assessment studies performed in gardens. Overall, this study has led to reflections and functional recommendations aimed at reducing human exposure and to finally developing sustainable gardening practices.

     

Exposure of domestic swine to influenza A viruses in Ghana suggests unidirectional,reverse zoonotic transmission at the human–animal interface

Influenza A viruses (IAVs) have both zoonotic and anthroponotic potential and are of public and veterinary importance. Swine are intermediate hosts and ‘mixing vessels’ for generating reassortants, progenies of which may harbour pandemic propensity. Swine handlers are at the highest risk of becoming infected with IAVs from swine but there is little information on the ecology of IAVs at the human–animal interface in Africa. We analysed and characterized nasal and throat swabs from swine and farmers respectively, for IAVs using RT‐qPCR, from swine farms in the Ashanti region, Ghana. Sera were also analysed for IAVs antibodies and serotyped using ELISA and HI assays. IAV was detected in 1.4% (n = 17/1,200) and 2.0% (n = 2/99) of swine and farmers samples, respectively. Viral subtypes H3N2 and H1N1pdm09 were found in human samples. All virus‐positive swine samples were subtyped as H1N1pdm09 phylogenetically clustering closely with H1N1pdm09 that circulated among humans during the study period. Phenotypic markers that confer sensitivity to Oseltamivir were found. Serological prevalence of IAVs in swine and farmers by ELISA was 3.2% (n = 38/1,200) and 18.2% (n = 18/99), respectively. Human H1N1pdm09 and H3N2 antibodies were found in both swine and farmers sera. Indigenous swine influenza A viruses and/or antibodies were not detected in swine or farmers samples. Majority (98%, n = 147/150) of farmers reported of not wearing surgical mask and few (4%, n = 6) reported to wear gloves when working. Most (n = 74, 87.7%) farmers reported of working on the farm when experiencing influenza‐like illness. Poor husbandry and biosafety practices of farmers could facilitate virus transmission across the human–swine interface. Farmers should be educated on the importance of good farm practices to mitigate influenza transmission at the human–animal interface.     

Differential gene expression and apoptosis markers in presymptomatic scrapie affected sheep

Bluetongue viruses (BTVs) could invade N-W Europe similar to BTV serotype 8 (BTV8/net06), since the source and route of introduction of this virus has not been solved. Therefore, the Dutch survey for Bluetongue by PCR testing was extended by further analysis of PCR positives to identify the involved BTV. In late August 2008, BTV was reported with 12 nucleotide differences in the S10 amplicon (S10 genotyping). This virus was identified as serotype 6, here named BTV6/net08. Promptly, serotype specific real-time PCR tests were developed for serotypes 1, 6, and 8 (S2 genotyping). Agreement was found between results by S10- and S2 genotyping. Further, BTV1 was identified by both S10- and S2 genotyping in one imported animal. After initial discovery of BTV6 in the Netherlands, animals from 18 holdings tested PCR positive for BTV6/net08 in 2008. Remarkably only one or two PCR positive animals per holding were found. Serum neutralization tests did not result in the discovery of more BTV6 infected animals. Retrospective studies indicated no evidence for infections by BTV6/net08 prior to the first discovery. Experimental infections with BTV6/net08 did not cause clinical disease in sheep, calves and cattle, except for a very short fever in some animals. This clearly showed that the vaccine-related BTV6/net08 is not virulent. BTV6/net08 was not found by passive and active surveys in the years after its discovery. Apparently, BTV6/net08 was not efficiently transmitted by endemic species of Culicoides in N-W Europe, and disappeared without the need of any control measure.     

Understanding pathogen population structure and virulence variation for efficient resistance breeding to control cucurbit powdery mildews

Cucurbit powdery mildew (CPM) is caused most frequently by well-differentiated obligate erysiphaceous ectoparasites Golovinomyces orontii and Podosphaera xanthii, which vary in their ecology and virulence. All economically important cucurbit crops host both of these CPM species. Breeding of cucurbits for CPM resistance is highly important worldwide, but adequate knowledge of CPM species determination, as well as virulence structure, population dynamics, and spatiotemporal variation of these pathogens, has not yet been achieved. New tools have been developed to enhance research on CPM virulence variation for more efficient breeding and seed and crop production. A set of differential genotypes of Cucumis melo, with high differentiation capacity, may contribute substantially to understanding of variation in CPM virulence at both individual and population levels. Long-term observations (2001–2012) of CPM pathogens in the Czech Republic were used to analyse virulence variation within and among annual CPM populations and demonstrate the utility of recently developed tools for studying species variability and virulence variation of CPM pathogens worldwide. Detailed analyses of diversity and spatiotemporal fluctuations in the composition of CPM populations provide crucial information for shaping breeding programmes and predicting the most effective sources of race-specific resistance. The primary aim of this work was to create a uniform framework for determination of CPM species structure and diversity, virulence phenotypes, virulence and phenotype frequencies, phenotype complexity, dynamics, and variation within and among CPM populations. In addition, practical advice is presented on how to select the most relevant data and interpret them for use in cucurbit resistance breeding.     

Control of stress-dependent cardiac growth and gene expression by a microRNA

The heart responds to diverse forms of stress by hypertrophic growth accompanied by fibrosis and eventual diminution of contractility, which results from down-regulation of alpha-myosin heavy chain (alphaMHC) and up-regulation of betaMHC, the primary contractile proteins of the heart. We found that a cardiac-specific microRNA (miR-208) encoded by an intron of the alphaMHC gene is required for cardiomyocyte hypertrophy, fibrosis, and expression of betaMHC in response to stress and hypothyroidism. Thus, the alphaMHC gene, in addition to encoding a major cardiac contractile protein, regulates cardiac growth and gene expression in response to stress and hormonal signaling through miR-208.     

A slowly progressive retinopathy in the Shetland Sheepdog

Objective To describe a slowly progressive retinopathy (SPR) in Shetland Sheepdogs. Animals Forty adult Shetlands Sheepdogs with ophthalmoscopic signs of SPR and six normal Shetland Sheepdogs were included in the study. Procedure Ophthalmic examination including slit‐lamp biomicroscopy and ophthalmoscopy was performed in all dogs. Electroretinograms and obstacle course‐test were performed in 13 affected and 6 normal dogs. The SPR dogs were subdivided into two groups according to their dark‐adapted b‐wave amplitudes. SPR1‐dogs had ophthalmoscopic signs of SPR, but normal dark‐adapted b‐wave amplitudes. Dogs with both ophthalmoscopic signs and subnormal, dark‐adapted b‐wave amplitudes were assigned to group SPR2. Eyes from two SPR2 dogs were obtained for microscopic examination. Results The ophthalmoscopic changes included bilateral, symmetrical, greyish discoloration in the peripheral tapetal fundus with normal or marginally attenuated vessels. Repeated examination showed that the ophthalmoscopic changes slowly spread across the central parts of the tapetal fundus, but did not progress to obvious neuroretinal thinning presenting as tapetal hyper‐reflectivity. The dogs did not appear seriously visually impaired. SPR2 showed significantly reduced b‐wave amplitudes throughout dark‐adaptation. Microscopy showed thinning of the outer nuclear layer and abnormal appearance of rod and cone outer segments. Testing for the progressive rod–cone degeneration ( prcd )‐mutation in three dogs with SPR was negative. Conclusion Slowly progressive retinopathy is a generalized rod–cone degeneration that on ophthalmoscopy looks similar to early stages of progressive retinal atrophy. The ophthalmoscopic findings are slowly progressive without tapetal hyper‐reflectivity. Visual impairment is not obvious and the electroretinogram is more subtly altered than in progressive retinal atrophy. The etiology remains unclear. SPR is not caused by the prcd‐mutation.     

Evidence of plaggen soils in European North Russia (Arkhangelsk region)

To clarify whether a particular group of soils of Archangelsk region (European N Russia) with humus‐rich topsoils exceeding the plowing zone supports an anthropogenic formation, four exemplary profiles were investigated. The investigation sites are characterized by distinct elevated surfaces, and the soils show thick toplayers of up to 60 cm with enrichment of soil organic matter and artifacts like brick, charcoal, and peat fragments, all indicating an anthropogenic origin. Increased phytolith amounts and high P contents of up to 800 mg kg^–1 citric acid–soluble P and up to 1,400 mg kg^–1 total P in the top horizons support an anthropogenic influence. These properties are very similar to the Plagganthrepts of NW Europe. The same is true regarding the main management aims: increasing soil fertility and overcoming the need of bedding materials. Having the required depths of the anthropogenic topsoil, the properties of the soils of the Archangelsk region allow a classification as Agrozems (Russian classification), Plaggenesche (German classification), and Plagganthrepts (US taxonomy). Since the high base saturation of the topsoil excludes a designation as plaggic horizon, the topsoil has to be considered as terric horizon, which leads to a classification as Terric Anthrosol according to WRB.     

Quantitation of odor-active compounds in rye flour and rye sourdough using stable isotope dilution assays

Application of the aroma extract dilution analysis on a flavor distillate prepared from freshly ground rye flour (type 1150) revealed 1-octen-3-one (mushroom-like), methional (cooked potato), and (E)-2-nonenal (fatty, green) with the highest flavor dilution (FD) factors among the 26 odor-active volatiles identified. Quantitative measurements performed by stable isotope dilution assays and a comparison to the odor thresholds of selected odorants in starch suggested methional, (E)-2-nonenal, and hexanal as contributors to the flour aroma, because their concentrations exceeded their odor thresholds by factors >100. Application of the same approach on a rye sourdough prepared from the same batch of flour revealed 3-methylbutanal, vanillin, 3-methylbutanoic acid, methional, (E,E)-2,4-decadienal, 2,3-butanedione, and acetic acid as important odorants; their concentrations exceeded their odor thresholds in water and starch by factors >100. A comparison of the concentrations of 20 odorants in rye flour and the sourdough made therefrom indicated that flour, besides the fermentation process, is an important source of aroma compounds in dough. However, 3-methylbutanol, acetic acid, and 2,3-butanedione were much increased during fermentation, whereas (E,E)-2,4-decadienal and 2-methylbutanal were decreased. Similar results were obtained for five different flours and sourdoughs, respectively, although the amounts of some odorants in the flour and the sourdough differed significantly within batches.