Lovastatin as a supplement to mitigate rumen methanogenesis: an overviewOA

Methane from enteric fermentation is the gas with the greatest environmental impact emitted by ruminants.Lovastatin(Lv) addition to feedstocks could be a strategy to mitigate rumen methane emissions via decreasing the population of methanogenic archaea(MA). Thus, this paper provides the first overview of the effects of Lv supplementation, focusing on the inhibition of methane production, rumen microbiota, and ruminal fermentation.Results indicated that Lv treatment had a strong anti-methanogenic...     

Solid-phase microextraction in the analysis of virgin olive oil volatile fraction: modifications induced by oxidation and suitable markers of oxidative status

Modifications of virgin olive oil subjected to accelerated storage were evaluated by HS-SPME analysis. To find a suitable marker of oxidative degradation, the volatile compounds showing variable concentration during the oxidative process have been identified and quantified by SPME coupled to GC-MS and GC-FID, respectively. The SPME analysis results were then compared with the parameters usually applied to assess the oxidative status of lipids, such as peroxide value, spectrophotometric absorbance, and loss of unsaturated fatty acids. Finally, the assessment of nonanal has been suggested as a marker of oxidative degradation. This rapid, inexpensive, and reliable method may allow screening of oils prior to testing by a panel of assessors.     

Descriptive report of antebrachial growth deformity correction in 17 dogs from 1999 to 2007, using hybrid linear-circular external fixator constructs

The radiographic and clinical results from 17 dogs (18 limbs) in which hybrid linear-circular external fixators (HLCEF) were used for the correction of antebrachial growth deformities (AGD) are described, retrospectively. Wedge ostectomy and the application of a type IA or IB HLCEF were performed in all cases. Pre- and postoperative AGD measurements, complications, time to staged destabilization and/or fixator removal, cosmesis, and limb function were evaluated. Complications included wire and/or pin tract inflammation (11 limbs) and radial fracture (2 limbs). All dogs had improved function and appearance after surgery. Limb function was assessed as excellent in 13 cases and good in 4 cases. Limb appearance was assessed as excellent in 12 cases and good in 5 cases. This case series substantiates the utility of HLCEF for the correction of AGD in dogs.     

New hosts for Lecanosticta acicola and Dothistroma septosporum in newly established arboreta in Spain

A historical outbreak of needle blight disease was recorded during 2018 to 2019 in plantations of Pinus radiata and Pinus nigra in the North of Spain. The main pathogens involved in this historical outbreak were identified as Lecanosticta acicola and Dothistroma septosporum. Recently, a variety of tree species in three arboreta planted between 2011 and 2013 in the Basque Country as part of the European project REINFFORCE were showing symptoms of needle blight and defoliation. The aim of this study was to determine which pine species were affected with these pathogens. Tree species sampled included several provenances of P. brutia, P. elliottii, P. nigra, P. pinaster, P. pinea, P. ponderosa, P. sylvestris and P. taeda. Using molecular identification methods, Lecanosticta acicola was confirmed infecting Pinus brutia (Provenance: Alexandropolis, Greece and var. eldarica, Crimea) and represents a new host species for this pathogen. Pinus elliottii (Provenance: Georgia, USA) and P. ponderosa (Provenance: Central California, USA) are new host reports of L. acicola for Spain. Dothistroma septosporum was found for the first time on P. brutia (Provenance: Marmaris, Turkey) and P. ponderosa (Provenance: Oregon, USA) in Spain and was also detected infecting P. nigra (Provenance: Sologne Vayrières, France).     

Micronutrient extraction in calcareous soils treated with humic concentrates

The use of humic concentrates in drip irrigation cultures in Spain has increased recently. Several companies offer different products to the farmers. It is suggested that such products are useful in improving the structure of the substrates, in increasing CEC of soils, in mobilizing micronutrients towards the roots and in acting as biological hormones. In our work, we have studied the effect of 11 different humic concentrates on extraction of iron and other micronutrients in the soil. Two substrates were used, a calcareous soil and a mixed substrate called “soil standard” since it has been made with components similar to those of a soil, but with standard materials. These soils were incubated with 11 different commercial humic concentrates. After the interaction, soils were extracted by the AB‐DTPA method. The results show that, in general, all commercial products increased extraction of micronutrients, but large amounts of humic concentrates were needed to increase them significantly. These quantities were greater than those reached in the bulk soil with the doses proposed by the distributors. However the amounts of humic substances used in this experiment can be reached in microsites near the droppers in a drip irrigation system. Although all humic concentrates increased micronutrients extraction, differences were noted on the efficiency of the different products. Humic rather than fulvic acids were responsible for this increase in extraction.     

Suture-related urolithiasis following repair of inadvertent prostatectomy in a dog

A 10-month-old male chow chow mixed breed dog was presented for anuria secondary to inadvertent prostatectomy performed during unilateral cryptorchidectomy. Surgical repair was successfully performed; however, this resulted in suture-associated urolith formation 3 months later, requiring a second surgical intervention and urethrostomy.     

Outcome associated with use of a percutaneously controlled hydraulic occluder for treatment of dogs with intrahepatic portosystemic shunts

OBJECTIVE: To evaluate efficacy of a hydraulic occluder (HO) used for treatment of dogs with an intrahepatic portosystemic shunt (IHPSS). DESIGN: Prospective study. ANIMALS: 10 dogs with an IHPSS. PROCEDURES: Serum biochemical and postprandial bile acids (PPBA) analyses and transcolonic scintigraphy were performed before surgery. Laparotomy was performed, and an uninflated HO was placed around the portal vein branch leading to the IHPSS. After surgery, 0.9% NaCl solution was injected into subcutaneous injection ports at 2, 4, 6, and 8 weeks to achieve staged occlusion of the HO. Serum biochemical analyses, PPBA analysis, and scintigraphy were performed 2 weeks after occlusion. Serum biochemical analyses were repeated 1 year after surgery. RESULTS: Implant revision was required in 3 dogs because of rupture of the HO (n = 2) or detachment of the actuating tubing (1). Serum biochemical values and clinical signs improved in all dogs after surgery. Six of 10 dogs had PPBA concentration within reference range 2 weeks after occlusion, and 2 additional dogs had concentrations within reference range at 1 year. Only 5 of 10 dogs had complete resolution of portosystemic shunting 2 weeks after occlusion. Two dogs were lost to follow-up, and 8 dogs remained alive with no recurrence of clinical signs at a median of 22 months after surgery. CONCLUSIONS AND CLINICAL RELEVANCE: Use of the HO appeared to be an effective method for surgical treatment for dogs with IHPSS, although problems with implant reliability indicate a need for modifications in design and manufacturing.