Using environmental factors to predict Rhipicephalus sanguineus s.l. (Acari: Ixodidae) mortality

Background

Rhipicephalus sanguineus s.l. (Latreille, 1806) can establish indoor populations, which increases the risk of pathogen transmission to humans and companion dogs. Rhipicephalus sanguineus s.l. ticks spend most of their life cycle off the host, which subjects developmental timescale to abiotic factors. Previous studies showed that both temperature and relative humidity (RH) influenced Rhipicephalus sanguineus s.l. survival time across all life stages. However, quantified relationships between environmental factors and Rhipicephalus sanguineus s.l. mortality is not currently available. Here, three Rhipicephalus sanguineus s.l. strains were evaluated for mortality under 20 combinations of five temperatures and four RHs. The data obtained were analyzed to quantify the relationship between environmental factors and Rhipicephalus sanguineus s.l. survival.

Results

Mortality probabilities did not show a consistent pattern between the three tick strains. Temperature, RH, and their interaction influenced Rhipicephalus sanguineus s.l. mortality probabilities across all life stages, with mortality probability generally increasing with temperature but decreasing with RH. With 50% and lower RH, larvae cannot survive for more than 1 week. However, mortality probabilities in all strains and stages were more sensitive to temperature than to RH.

Conclusion

This study identified the predictive relationship between environmental factors and Rhipicephalus sanguineus s.l. survival, which enables estimations of tick survival time under varied residential situations, allows parameterization of population models, and provides guidance for pest control professionals to develop efficient management strategies. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.     

Search and identification methods that owners use to find a lost cat

OBJECTIVE: To characterize the process by which owners search for lost cats and identify factors associated with time to recovery. DESIGN: Cross-sectional study. SAMPLE POPULATION: Owners of 138 cats lost in Montgomery County, Ohio, between June 1 and September 30, 2005. PROCEDURES: A telephone survey was conducted. RESULTS: 73 of the 138 (53%) cats were recovered; median time to recovery was 5 days (range, 0.5 to 81 days). Most cats (48 [66%]) that were recovered returned home on their own or were found in the neighborhood (5 [7%]); most other cats were recovered through posting of neighborhood signs (8 [11%]) or calling or visiting an animal agency (5 [7%]). The highest success rate for any of the search methods that were used was only 12% (posting neighborhood signs). Only 26 of the 138 (19%) cats had some type of identification at the time they were lost (ie, identification tag, rabies tag, or microchip). Owners allowed 82 (59%) cats to spend at least some time outdoors. The percentage of sexually intact cats recovered by their owners (4/16 [25%]) was significantly lower than the percentage of neutered cats recovered (69/122 [57%]). CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that the percentage of lost cats recovered by their owners is low, possibly in part because of the lack of use of traditional identification methods and the general acceptance that cats may roam. Veterinarians can help educate owners about the importance of identification and the need to keep cats indoors.     

Search and identification methods that owners use to find a lost dog

OBJECTIVE: To characterize the process by which owners search for lost dogs and identify factors associated with time to recovery. DESIGN: Cross-sectional study. SAMPLE POPULATION: Owners of 187 dogs lost in Montgomery County, Ohio, between June 1 and September 30, 2005. PROCEDURES: A telephone survey was conducted. RESULTS: 132 of the 187 (71%) dogs were recovered; median time to recovery was 2 days (range, 0.5 to 21 days). Dogs were recovered primarily through a call or visit to an animal agency (46 [34.8%]), a dog license tag (24 [18.2%]), and posting of neighborhood signs (20 [15.2%]). Eighty-nine (48%) dogs had some type of identification at the time they were lost (ie, identification tag, dog license tag, rabies tag, or microchip). Owners had a higher likelihood of recovery when they called an animal agency (hazard ratio, 2.1), visited an animal agency (1.8), and posted neighborhood signs. Dogs that were wearing a dog license tag also had a higher likelihood of recovery (hazard ratio, 1.6). Owners were less likely to recover their dogs if they believed their dogs were stolen (hazard ratio, 0.3). CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that various factors are associated with the likelihood that owners will recover a lost dog. Both animal agencies and veterinarians can play a role in educating dog owners on the importance of identification tags, licensing, and microchips and can help to emphasize the importance of having a search plan in case a dog is lost.     

Comparison of COAP and UW-19 protocols for dogs with multicentric lymphoma

BACKGROUND: Various chemotherapy protocols for treating lymphoma in dogs have been published; however, comparison of protocols from different studies is difficult, especially when evaluating survival time and toxicoses. HYPOTHESIS: The choice of COAP (C, cyclophosphamide; O, vincristine; A, cytosine arabinoside; P, prednisone) and a modified University of Wisconsin 19-week (UW-19) induction protocol has no influence on overall survival times in dogs with lymphoma. ANIMALS: One hundred and one dogs with multicentric lymphoma. METHODS: Retrospective study (2001-2006). Dogs induced with either an 8-week COP-based protocol (C, cyclophosphamide; O, vincristine; and P, prednisone) with maintenance therapy (COAP group) or a 19-week CHOP (C, cyclophosphamide; H, doxorubicin; O, vincristine; and P, prednisone) based protocol (UW-19 group) were compared in terms of the duration of first remission, survival time, toxicoses, and cost. RESULTS: There were 71 dogs in the COAP group and 30 dogs in the UW-19 group. Various protocols were used after the first relapse. The median duration of the first remission for the COAP and UW-19 groups were 94 days (range, 6-356 days) and 174 days (28-438 days), respectively (P < .01). The median survival times for dogs in the COAP and UW-19 groups were 309 days (6-620 days) and 275 days (70-1102+ days), respectively (P = .09). Dogs in the COAP group had a hazard ratio of 1.9 (95% CI 1.1-3.4) for death relative to the UW-19 group (P = .03), after controlling for the confounders (World Health Organization clinical stage, age, sex, use of doxorubicin during reinduction). The severity of neutropenia and gastrointestinal toxicoses were significantly higher in the UW-19 group than in the COAP group (P = .01 and P < .01, respectively). CONCLUSION AND CLINICAL IMPORTANCE: Use of a long-term doxorubicin-containing sequential combination chemotherapy protocol is associated with a decreased risk of relapse and death relative to a non-doxorubicin-containing protocol.     

Insights on endemism: comparison of the duration of the marine larval phase estimated by otolith microstructural analysis of three amphidromous Sicyopterus species (Gobioidei: Sicydiinae) from Vanuatu and New Caledonia

Lord C, Brun C, Hautecœur M, Keith P. Insights on endemism: comparison of the duration of the marine larval phase estimated by otolith microstructural analysis of three amphidromous Sicyopterus species (Gobioidei: Sicydiinae) from Vanuatu and New Caledonia.
Ecology of Freshwater Fish 2010: 19: 26–38. © 2009 John Wiley & Sons A/S
Abstract –  The microstructure of otoliths of three amphidromous gobies (Sicydiinae) of the genus Sicyopterus has been analysed. One of these species, Sicyopterus lagocephalus has a widespread distribution whereas the other two are endemic ( Sicyopterus aiensis , from Vanuatu and Sicyopterus sarasini , from New Caledonia). The microstructural analysis of the otoliths showed that the duration of the marine larval phase is significantly shorter for both endemic species than for the cosmopolitan species (131 ± 3.4 days for S. lagocephalus , 79.2 ± 4.6 days for S. aiensis and 76.5 ± 3.9 days for S. sarasini ). These results led us to infer that it could be one of the factors explaining endemism. We have also improved our knowledge on the processes undergone during the marine phase by studying the growth of the otolith. This work allowed us to propose possible migration routes. These species' reproduction period has also been studied by back-calculating hatching dates. All these elements improve our understanding of the life-history traits and dispersion strategies of these species, which is crucial to implementing conservation measures in order to protect them and their environment, both threatened by anthropogenic actions.     

Predicting nitrogen availability and losses following application of organic manures to arable land: MANNER

Abstract. A decision support system to predict the plant availability of nitrogen (N) following organic manure applications to land has been developed, drawing together the latest UK research information on factors affecting manure N availability and losses. The ADAS MAN ure N itrogen E valuation R outine (MANNER) accounts for manure N analysis, ammonia volatilization, nitrate leaching and mineralization of manure organic N. Only a few easily available inputs are required to predict the amount of N volatilized or leached, and the fertilizer N value for the next crop grown. Predictions from MANNER have been evaluated by comparison with independently collected data from a range of experimental studies where pig, cattle and poultry manures were applied to arable crops. Good agreement was found ( r ² 60–79%, P <0.001), confirming that MANNER can provide a reliable estimate of the fertilizer N value of farm manures spread to arable land under a range of conditions.     

Effects of preadoption counseling for owners on house-training success among dogs acquired from shelters

OBJECTIVE: To determine the effect of preadoption counseling for owners on house-training success among dogs acquired from shelters. DESIGN: Prospective study. SAMPLE POPULATION: 113 dog owners. PROCEDURES: Participants were randomly assigned to a treatment (n = 54) or a control (59) group. Dog owners in the treatment group received counseling (5 minutes' duration) regarding house-training. Owners in the control group did not receive counseling, but all other adoption procedures were otherwise identical to those applied to the treatment group. All participants were contacted by telephone 1 month after adoption of a dog for assessment of house-training status and related issues by use of a standardized survey method; data were compared between groups. RESULTS: Most shelter dogs were considered successfully house-trained by their owners 1 month after adoption. Furthermore, dogs were considered house-trained by significantly more owners who received preadoption counseling than control group owners (98.1% vs 86.4%). Owners who received counseling used verbal punishment on their dogs during house-training less frequently and applied enzymatic cleaners to urine- or feces-soiled areas more frequently than owners in the control group. CONCLUSIONS AND CLINICAL RELEVANCE: Results have suggested that brief preadoption counseling for owners enhances successful house-training of dogs adopted from shelters. Counseling owners at the time of pet acquisition may thus have beneficial effects in the prevention of inappropriate elimination behaviors. Veterinarians and animal care staff should be encouraged to devote time to counsel new pet owners on successful house-training, as well as other healthcare and behavioral needs.     

Genome of the host-cell transforming parasite Theileria annulata compared with T. parva

Theileria annulata and T. parva are closely related protozoan parasites that cause lymphoproliferative diseases of cattle. We sequenced the genome of T. annulata and compared it with that of T. parva to understand the mechanisms underlying transformation and tropism. Despite high conservation of gene sequences and synteny, the analysis reveals unequally expanded gene families and species-specific genes. We also identify divergent families of putative secreted polypeptides that may reduce immune recognition, candidate regulators of host-cell transformation, and a Theileria-specific protein domain [frequently associated in Theileria (FAINT)] present in a large number of secreted proteins.     

Congestive heart failure in two pet rabbits

This case report describes congestive heart failure with pleural effusion in two middle-aged, pet house rabbits. Both had a history of acute onset dyspnoea, weakness and weight loss. Bi-atrial enlargement was seen on echocardiography in both rabbits. One rabbit had atrial fibrillation and ventricular premature complexes identified on electrocardiography. There was a radiographically evident pleural effusion in both rabbits and thoracocentesis was undertaken in one rabbit. These findings were confirmed on post-mortem examination. The aetiology for the underlying heart disease was not found, but the potential types of cardiomyopathies are discussed.