Possibilities for harmonizing national forest inventory data for use in forest biodiversity assessments

Representatives of the national forest inventories (NFIs) of27 European countries and the USA evaluated possibilities fora common approach to forest biodiversity reporting. The projectwas conducted under the auspices of COST (European Cooperationin the field of Scientific and Technical Research) Action E43,‘Harmonisation of National Forest Inventories in Europe:Techniques for Common Reporting’. Based on the analysisof responses to an initial questionnaire regarding the mostecologically important and technically feasible variables formonitoring forest biodiversity using NFI data, 16 key variableswere selected. Responses to a second questionnaire consistingof six questions regarding assessment of the 16 key variablesrevealed that the NFIs of most responding countries alreadyassess a large proportion of these variables. Each of the 16variables is assessed in at least eight NFIs. However, the responsesregarding assessment methods and necessary field crew expertisevaried considerably. As a first example, although tree diametersat breast height (d.b.h.) are assessed by every country, thereis a wide range of minimum d.b.h. thresholds from 0 mm in Finlandto 12 cm in Cyprus and Switzerland. As a second example, eightcountries estimated that almost all inventory variables requirehighly skilled forest staff with specialized training, but mostcountries agree that a middle level of expertise is sufficient.Despite differences in current NFI methods, overall, the resultsof the study indicate positive possibilities for achieving amoderate level of European-wide harmonization.     

A transient radio jet in an erupting dwarf nova

Astrophysical jets seem to occur in nearly all types of accreting objects, from supermassive black holes to young stellar objects. On the basis of x-ray binaries, a unified scenario describing the disc/jet coupling has evolved and been extended to many accreting objects. The only major exceptions are thought to be cataclysmic variables: Dwarf novae, weakly accreting white dwarfs, show similar outburst behavior to x-ray binaries, but no jet has yet been detected. Here we present radio observations of a dwarf nova in outburst showing variable flat-spectrum radio emission that is best explained as synchrotron emission originating in a transient jet. Both the inferred jet power and the relation to the outburst cycle are analogous to those seen in x-ray binaries, suggesting that the disc/jet coupling mechanism is ubiquitous.     

Membrane fusion intermediates via directional and full assembly of the SNARE complex

Cellular membrane fusion is thought to proceed through intermediates including docking of apposed lipid bilayers, merging of proximal leaflets to form a hemifusion diaphragm, and fusion pore opening. A membrane-bridging four-helix complex of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) mediates fusion. However, how assembly of the SNARE complex generates docking and other fusion intermediates is unknown. Using a cell-free reaction, we identified intermediates visually and then arrested the SNARE fusion machinery when fusion was about to begin. Partial and directional assembly of SNAREs tightly docked bilayers, but efficient fusion and an extended form of hemifusion required assembly beyond the core complex to the membrane-connecting linkers. We propose that straining of lipids at the edges of an extended docking zone initiates fusion.     

Challenges to Organic Potato Farming: Disease and Nutrient Management

For organic potato producers the two main challenges are disease and nutrient management. Both factors are limited by regulations that on the one hand prohibit the use of chemical fertilisers, especially nitrogen and, on the other hand, most synthetic pesticides. Late blight, caused by Phytophthora infestans is commonly thought to be the factor most limiting yield. However, because there is no really effective fungicide available to control late blight, there are virtually no yield loss data available for organic farming conditions. In this paper the state of the art of organic potato management with respect to disease and nutrient management is summarised. In a second part, the interactive effects of N-availability in the soil, climatic conditions and late blight were studied in the presence and absence of copper fungicides from 2002–2004 for the mid-early main-crop potato cv. Nicola. From the experimental work it became clear that copper fungicides in most cases do slow down epidemics adding an average of 3 days to the growth duration. However, only 30% of the variation in yield could be attributed to disease reduction. A model including disease reduction, growth duration and temperature sum from planting until 60% disease severity was reached, and soil mineral N contents at 10 days after emergence could explain 75% of the observed variation in yield. However, the model failed when N-supply was extremely high. The implications of the results on the management of organic potatoes with respect to cultivar choice, nutrient and disease management are discussed. In conclusion, several points emerge from the results: In organic farming, yields are foremost limited by nutrient availability in spring and early summer. The effects of late blight on yields may often be overestimated and cannot be deducted from results in conventional farming because of the strong interaction with nutrient status. Resistance clearly remains the most important strategy against late blight in organic potato production. However, as important or even more important than resistance is the early development and bulking behaviour and the ability of a cultivar to make use of organic nutrients efficiently. In the absence of efficient organic pesticides it is possible to reduce blight pressure to a certain extent by arranging the crop in small narrow fields perpendicular to the main wind direction neighboured either by non-hosts or completely resistant potatoes.     

Effect of <Emphasis Type="Italic">Vachellia tortilis</Emphasis> on understory vegetation,herbaceous biomass and soil nutrients along a grazing gradient in a semi-arid African savanna

The spatial pattern and abundance of herbaceous vegetation in semi-arid savannas are dictated by a complex and dynamic interaction between trees and grasses. Scattered trees alter the composition and spatial distribution of herbaceous vegetation under their canopies. Therefore, we studied the effect of Vachellia tortilis on herbaceous vegetation composition, biomass and basal area, and soil nutrients on sites with varying grazing intensities in the central rift valley of Ethiopia. Data were collected on species composition, cover and biomass of herbs and grasses, and soil moisture and nutrient contents under light, medium, and heavy grazing pressures, both under the inside and outside of V. tortilis canopies. Species richness was similar in both locations but decreased with increased grazing. Only the overall biomass and herb cover were significantly greater under the canopy than outside, and overall biomass showed significant unchanging decline with increased grazing. However, vegetation cover was significantly greater on moderately grazed sites compared to low and heavily grazed sites. All soil variables were significantly higher under V. tortilis canopies than outside. Our findings suggest that V. tortilis has more effect on composition and diversity of herbaceous vegetation than on species richness, and that V. tortilis promotes the herbaceous layer biomass by reducing soil moisture loss and increasing soil fertility under the inside than outside the canopies. Therefore, we suggest that management practices should be directed on reducing pressure on V. tortilis by regulating grazing. Low to moderate grazing levels (i.e., a stocking rate less than 39.6 TLU ha^?1 yr^?1) seems to be tolerable to ensure sustainable conservation of the species in the study area in particular and in semi-arid savannas in general.     

Immunohistochemical localization of androgen and oestrogen receptors in canine hair follicles

Skin biopsies from seven different body sites were obtained from 21 dogs of different breeds (short, normal, long hair), which were presented for euthanasia. Commercially available polyclonal antibodies were used for the immunohistochemical detection of androgen and oestrogen receptors. Both receptors showed a similar distribution in canine skin, with specific intranuclear staining. In the epidermis, the percentage of androgen receptor (AR)-positive cells, but not that of oestrogen receptor (ER)-positive cells, was significantly higher in samples from the thorax and the flank. In the dermal papilla, the percentage of ER-positive (but not AR-positive) cells was significantly lower in biopsies from the flank. No significant difference was found for both receptors between the locations in the outer root sheath, among the three different hair types, between sex and between intact and castrated dogs.     

Primary cell culture and morphological characterization of canine dermal papilla cells and dermal fibroblasts

Skin biopsies were taken from female dogs, the primary hair follicles isolated and the dermal papilla dissected. After incubation in supplemented Amniomax complete C100 medium in 24-well culture plates, the dermal papilla cells (DPC) grew to confluence within 3 weeks. Thereafter, they were subcultivated every 7 days. Dermal fibroblast (DFB) cultures were established by explant culture of interfollicular dermis in serum-free medium, where they reached confluence in 10 days. They were subcultivated every 5 days. For immunohistochemistry, cells were grown on cover slips for 24 h, fixed and stained with antibodies against collagen IV and laminin. DPC showed an aggregative growth pattern and formation of pseudopapillae. Intensive staining for collagen IV and laminin could be observed until the sixth passage. DFB grew as branching, parallel lines and showed only weak staining for collagen IV and laminin.