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91.
El Tigani A. Asil Sulieman M. El Sanousi Ahmed Gameel Haytham El Beir Maha Fathelrahman Nasir M. Terab Magzoub A. Muaz Mohamed E. Hamid 《Tropical animal health and production》2013,45(2):469-472
Bovine tuberculosis (BTB) is a widespread zoonosis in developing countries but has received little attention in many sub-Saharan African countries including Sudan and particularly in some parts such as Darfur states. This study aimed to detect bovine tuberculosis among caseous materials of cattle slaughtered in abattoirs in South Darfur State, Sudan by using microscopic and PCR-based methods. The study was a cross-sectional abattoir-based study which examined a total of 6,680 bovine carcasses for caseous lesions in South Darfur State between 2007 and 2009. Collected specimens were examined for the presence of acid-fast bacilli (AFB) by using microscopic and culture techniques. Isolated mycobacteria were identified by selected conventional cultural and biochemical tests in comparison to a single tube multiplex PCR (m-PCR) assay which detect Mycobacterium bovis-specific 168-bp amplicons. Of the total 6,680 slaughtered cattle examined in South Darfur, 400 (6 %) showed caseations restricted to lymph nodes (86.8 %) or generalized (13.2 %). Bovine tuberculosis was diagnosed in 12 (0.18 %), bovine farcy in 59 (0.88 %), unidentified mycobacteria in 6 (0.09 %), and missed or contaminated cultures in 7 (0.1 %). Out of 18 cultures with nonbranching acid-fast rods, 12 amplified unique 168-bp sequence specific for M. bovis and subsequently confirmed as M. bovis. With the exception of the reference M. tuberculosis strains, none of the remaining AFB amplified the 337-bp amplicon specific for M. tuberculosis. It could be concluded that bovine tuberculosis is prevalent among cattle in South Darfur representing 4.5 % from all slaughtered cattle with caseous lesions. The study sustains microscopy as a useful and accessible technique for detecting AFB. m-PCR assay proved to be valuable for confirmation of BTB and its differentiation from other related mycobacteriosis, notably bovine farcy. 相似文献
92.
Nahid Y. El Sayed Ekhlas M. Abdelbari O. M. Mahmoud S. E. I. Adam 《The Veterinary quarterly》2013,33(2):80-85
Ten Nubian goats were given oral doses of the fresh fruits and leaves of Cassia senna at 1, 5, and 10 g / kg /day. Eight goats died within 30 days and two others were slaughtered in a moribund condition on days 18 and 29. The clinical signs of diarrhoea, inappetence, loss of condition, and dyspnoea were well correlated with the pathological findings. There was an increase in G.O.T., ammonia, urea, and total cholesterol and a decrease in total protein in the serum of Cassia‐poisoned goats. Blood sugar level was reduced and the increase in the values of Hb, PCV, and RBC was due to haemoconcentration. 相似文献
93.
Organic wastes can be usefully recovered to produce organic amendments, for example, compost, to be used for crop production, thus reducing impacts through efficient waste management. The aim of this work was to study the effects of compost obtained from municipal waste in combination with poultry manure on plant growth, nitrate reductase (NR) activity and absorption and distribution of heavy metals (HM) in plant tissues of tomatoes, grown in pots in greenhouses. Two compost types obtained from municipal waste mixed with poultry manure (C1?=?3:2 and C2?=?2:3) were used at two different ages (105 d and 173 d) and at two mix rates with soil (32.5?g pot?1 and 65?g pot?1); soil with no compost amendment was used as control. The experiment was conducted using tomato plants in pots and plant growth and nutrient plant uptake was determined after 65?days from plant transplanting. Results obtained indicated that compost type and compost rate affected biomass production. However, compost age did not influence the development of plants. Nutrient status of tomato plants was also investigated with reference to the N cycle. Nitrite accumulation in the leaves increased with the increase in compost doses. The accumulation of NO2? was associated with a significant increase in NR activity. HM content in leaves decreased with compost use. HM accumulated preferentially in roots and leaves and the soil to root metals transfer was in this order: Fe (1.08–2.14)> Co (0.53–4.10)>Cu (0.28–2.28) >Mn (0.3–1.34) >Zn (0.87–1.21)>Cr (0.12–1.64). The highest and lowest dynamic bioaccumulation factors (BAFdyn) were observed in roots and stems, respectively. The root system acted as a barrier for Cd and Pb. It was concluded that compost use is beneficial for tomato plants, with particular reference to the compost obtained by using a higher amount of poultry manure (C2) in the mix. 相似文献
94.
Contribution of technic materials to the mobile fraction of metals in urban soils in Marrakech (Morocco) 总被引:5,自引:2,他引:3
Hicham El Khalil Christophe Schwartz Ouafae Elhamiani Jochen Kubiniok Jean Louis Morel Ali Boularbah 《Journal of Soils and Sediments》2008,8(1):17-22
Background, Aim and Scope In urban areas, soils are often dramatically altered by anthropogenic activity and these modifications distinguish these soils
(Anthrosols, Technosols) from those in natural systems. In urban environments, they receive considerable pollution from industry,
traffic and refuse. Since contaminated soil particles can be easily inhaled or ingested, there is a potential transfer of
toxic pollutants to humans. Risk assessment is essentially based on the determination of the total or mobile contents of pollutants
in soils using chemical extractions. This approach could be improved by taking into consideration the bioavailable fractions
of these toxic elements as measured by biotests. The coarse soil fraction usually neglected in analyses can nevertheless have
an effect on the concentration of metals in the soil solution. This coarse fraction is made up of the natural materials and
of technic materials constituting anthropogenic soils (plastic, paper, fabric, wood, bones, metallic elements and building
materials). These materials have variable capacities to release or adsorb trace elements. Samples representative of different
technic fraction components of Marrakech urban soils permit one to quantify their contribution to the enrichment of the soluble
metal concentrations. Works are carried out to achieve partial extractions of metals from the three fractions (less than 2
mm, coarse natural and coarse technic) of selected urban soils in order to determine their contribution to the metal contamination
of soils.
Materials and Methods Selected soils were collected from 9 sites according to a gradient of increasing anthropogenic influence from suburban to
urban zones. Soils were air-dried, homogenized, and sieved (2 mm). The coarse fraction was sorted to separate the different
technic materials and natural materials. Water extractions were run, on the natural, coarse fraction, on the complete technic
fraction of the 9 soils and on average samples made of technic materials sorted out of 58 topsoils sampled from different
sites in the city of Marrakech.
Results Results show that the percentage of the technic fraction increases while approaching the historic city center. It represented
about 14% in the most anthropogenically disturbed soils. Along this gradient, soils changed progressively from Anthrosols
to Technosols according to the WRB classification of urban and industrial soils. Analyses of metal contents showed that the
fine fraction (<2 mm) mainly contributed to the metallic contamination of the water soluble fraction. The natural coarse fraction
had the highest contribution to the copper release and was responsible for the release of all water-extractable copper in
some soils. Concerning the technic fraction, it has a significant contribution essentially in the most anthropogenically disturbed
soils as characterized by an elevated percentage of anthropogenic elements. The water extractable metal contents of average
samples of these anthropogenic elements shows that elevated metal concentrations were released by bones, wood, plastic and
fabric/paper.
Discussion This study concerns soils in urban areas, which are strongly impacted by human activities. Part of the soils can be classified
as Anthrosols, profoundly impacted through the addition of organic materials from household wastes, irrigation, or cultivation.
Other soils strongly impacted by human activities are Technosols dominated or strongly influenced by man-made materials. Technosols
appear mostly in urban and industrial areas and are more likely to be contaminated than Anthrosols. The composition and heterogeneity
of urban soils lead to modifications of the mobility and availability of pollutants depending on successive land-uses and
on the composition of technic materials. The fine fraction offers a high transferring surface capacity, leading to a high
mobilization of metals. The technic fraction contributes significantly to the metal release in the Technosols. This property
can be explained by a reversible adsorption of metals on the organic matter.
Conclusions Results confirm that anthropogenic activity causes a wide spatial diversity of soil quality in the urban and suburban area.
It introduces large amounts of technic materials in soils that could have an impact on the metal availability. It therefore
acts on the metal bioavailability in the urban Technosols.
Recommendations and Perspectives These results show that it is necessary, in addition to the characterization of the fine particles, to take into account the
contribution of the coarse fraction of the Technosols in the evaluation of risks of transfer of metals to the food chain. 相似文献
95.
Chaouachi M El Malki R Berard A Romaniuk M Laval V Brunel D Bertheau Y 《Journal of agricultural and food chemistry》2008,56(6):1818-1828
The labeling of products containing genetically modified organisms (GMO) is linked to their quantification since a threshold for the presence of fortuitous GMOs in food has been established. This threshold is calculated from a combination of two absolute quantification values: one for the specific GMO target and the second for an endogenous reference gene specific to the taxon. Thus, the development of reliable methods to quantify GMOs using endogenous reference genes in complex matrixes such as food and feed is needed. Plant identification can be difficult in the case of closely related taxa, which moreover are subject to introgression events. Based on the homology of beta-fructosidase sequences obtained from public databases, two couples of consensus primers were designed for the detection, quantification, and differentiation of four Solanaceae: potato (Solanum tuberosum), tomato (Solanum lycopersicum), pepper (Capsicum annuum), and eggplant (Solanum melongena). Sequence variability was studied first using lines and cultivars (intraspecies sequence variability), then using taxa involved in gene introgressions, and finally, using taxonomically close taxa (interspecies sequence variability). This study allowed us to design four highly specific TaqMan-MGB probes. A duplex real time PCR assay was developed for simultaneous quantification of tomato and potato. For eggplant and pepper, only simplex real time PCR tests were developed. The results demonstrated the high specificity and sensitivity of the assays. We therefore conclude that beta-fructosidase can be used as an endogenous reference gene for GMO analysis. 相似文献
96.
V. A. Demkin N. N. Kashirskaya T. S. Demkina T. E. Khomutova M. V. El’tsov 《Eurasian Soil Science》2008,41(2):115-127
Paleosols buried under kurgans of the Bronze (end of the fourth and the third millennia BC), Early Iron (1st–3rd centuries AD), and Medieval (13th century AD) epochs have been studied on the Ilovlya River (a tributary of the Don River) terrace. The evolution of chestnut soils in the south of the Privolzhskaya Upland during the last 5000 years has been traced. It is shown that the mean weighted contents and distribution of soluble salts, gypsum, and carbonates in the soil profiles have been subjected to cyclic changes. The total microbial biomass and its trophic structure in the A1, B1, and B2k horizons of paleosols of different ages have been determined. A comparative analysis of the morphological, chemical, and microbiological data on the paleosols of different ages has been used to reconstruct the climatic dynamics for the last 50 centuries. The aridity of the climate in the studied region increased at the end of the third-the beginning of the second millennia BC and in the second and third centuries AD. The humidization of the climate took place in the 1st and in the 12th–13th centuries AD. 相似文献
97.
N. El Azhari D. Bru A. Sarr & F. Martin-Laurent 《European Journal of Soil Science》2008,59(4):665-673
The β-ketoadipate pathway is the major route for degradation of aromatic compounds by various soil microorganisms. Protocatechuate 3,4-dioxygenase, a key enzyme of this pathway and which is encoded by pcaGH genes, catalyses the ring cleavage of protocatechuate. Microorganisms harbouring pcaGH genes are widely distributed in the environment but little is known about their relative abundance within the total microflora. Hence, this paper reports the development of a real-time PCR assay to quantify the bacterial pcaH sequence that encodes the β sub-unit of the protocatechuate 3,4-dioxygenase. This real-time PCR assay was linear over seven orders of magnitude with a calculated efficiency of 99% and sensitive up to 102 copies of the pcaH sequence per assay. Real-time PCR analyses performed on six soils with different physico-chemical properties, revealed pcaH densities ranging from 103 to 104 copies of pcaH ng−1 of soil DNA, which corresponded to approximately 0.2–10.9% of the total bacterial community. The sequencing of real-time PCR amplicons yielded 48 deduced amino acid sequences that exhibited 44–100% identity to known bacterial PcaH sequences, thereby confirming the accuracy of this real-time PCR assay. 相似文献
98.
Salih DA Julla II Hassan SM El Hussein AM Jongejan F 《The Onderstepoort journal of veterinary research》2008,75(1):47-53
In a preliminary survey conducted in 2005, the species composition and seasonality of ticks infesting cattle in Central Equatoria State, Southern Sudan was determined. Three locations were selected (Gumbo, Khor Rumla and Nyaing) and surveyed every 3 months. Two cattle herds in each of the three locations were visited four times during the study period. Total body collections of ticks were made from each of five cattle (Nilotic Zebu breed) kept in six different herds. Four tick genera and ten species were identified. The tick species identified were Amblyomma lepidum, Amblyomma variegatum, Boophilus annulatus, Boophilus decoloratus, Hyalomma marginatum rufipes, Hyalomma truncatum, Rhipicephalus appendiculatus, Rhipicephalus evertsi evertsi, Rhipicephalus praetextatus and Rhipicephalus sanguineus group. The highest number of ticks was collected in October during the rainy season. A finding of great significance was that R. appendiculatus, vector of East Coast fever, has now firmly established itself throughout the year with possible implications for cattle production in Central Equatoria State. 相似文献
99.
Zilberstein LF Moens YP Leterrier E 《Veterinary journal (London, England : 1997)》2008,178(2):214-218
A dose of supplementary ketamine was used to evaluate the anaesthetic sparing effect of adding local anaesthesia to general anaesthesia in cats undergoing ovariectomy. Fifty-six healthy cats were randomly assigned to receive lidocaine 2% (group L) as skin infiltration (1 mg kg(-1)), topical application (splash block) on both the ovaries (2 mg kg(-1), each) and on abdominal muscular layers (1 mg kg(-1)), or an equal volume of NaCl 0.9% at the same sites (group S). Anaesthesia was induced with a mixture of 20 microg kg(-1) medetomidine and 5 mg kg(-1) ketamine administered intramuscularly. Rectal temperature, ECG, heart rate and respiratory rate were measured continuously. Ketamine supplemental boli (1 mg kg(-1), intravenously) were administered in response to movements during surgery. Local lidocaine significantly reduced the need for supplementary ketamine. All animals were returned to their owners without complications. With this protocol, local anaesthetics reduced the need for injectable anaesthetic during feline ovariectomy. 相似文献
100.
Youssef A. Attia Fulvia Bovera Abd El‐Hamid Abd Elhamid Sameer A. Nagadi Mohamed A. Mandour Saber Sh. Hassan 《Journal of animal physiology and animal nutrition》2019,103(3):959-968
To evaluate the effect of bee pollen (BP) and/or propolis (Pro) supplementation on rabbit does, 64 nulliparous NZW rabbits does were distributed among eight groups (eight animals/group). One unsupplemented group was the control; the other seven groups were supplemented, respectively, with zinc bacitracin (ZnB) at 100 mg, BP at 150 and 300 mg, Pro at 150 and 300 mg, BP+Pro at 150 and 300 mg of each three times/week, day after day continuously along eight parities. The BP300, Pro300 and BP+Pro150 groups had higher body weight of litter at birth and number of kids born alive. The BP supplementation at 150 mg increased plasma total protein and albumin than the control group. The BP or Pro at 150 mg decreased plasma T3 than the other groups except for BP+Pro150. The ZnB group had significantly greater T3/T4 ratio compared to BP, Pro and BP+Pro at 150 mg. The BP+Pro150 group had less ALT than the control; BP300 and Pro 300 mg resulted in lower plasma AST than the groups Pro150 with or without BP and the control group. The plasma alkaline phosphatase of BP at 150 or 300 mg and BP+Pro150 was significantly greater than that of the Pro150 group. The BP+Pro300 group had higher WBCs than the other groups. In contrast, the lymphocytes were greater in the Pro and BP+Pro300 groups than in BP, Pro and BP+Pro at 150 mg. The groups supplemented with BP and BP+Pro at 150 and 300 mg had significantly greater SRBCs of doe rabbits and their offspring compared to the control and the ZnB group. The BP at 300 mg increased the serum albumin and α1‐globulin than the control group. The Pro300 group had greater serum α2‐globulin and β‐globulin than the control group. The total globulin was significantly greater for the 300 mg propolis‐supplemented groups than the control. 相似文献