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41.
Kazuhiro TAMURA Mikihiro YOSHIE Keisuke HASHIMOTO Eiichi TACHIKAWA 《The Journal of reproduction and development》2014,60(6):447-453
Angiogenesis in the developing corpus luteum (CL) is a prerequisite for establishment and maintenance of an early pregnancy. To explore the physiological significance of insulin-like growth factor-binding protein-7 (IGFBP7) in the developing CL, the effects of IGFBP7 on vascular endothelial growth factor (VEGFA)- and luteinizing hormone (LH)-induced in vitro tube formation were tested using isolated luteal microvascular endothelial cells (LECs). Capillary-like tube formation of LECs and their proliferation were stimulated by both VEGFA and LH. IGFBP7 treatment suppressed VEGFA- or LH-induced tube formation. The proliferation and migration of LECs, and phosphorylation of mitogen-activated protein kinase kinase and extracellular signal-regulated kinase 1/2 were inhibited by IGFBP7. Furthermore, IGFBP7 attenuated VEGFA-enhanced cyclooxygenase (COX)-2 mRNA expression and prostaglandin E2 secretion. These findings suggest the possibility that
luteal IGFBP7 secretion may suppress the stimulatory effect of VEGFA on angiogenesis in the early CL. 相似文献
42.
43.
Harada Y Takagaki Y Sunagawa M Saito T Yamada L Taniguchi H Shoguchi E Sawada H 《Science (New York, N.Y.)》2008,320(5875):548-550
Hermaphroditic organisms avoid inbreeding by a system of self-incompatibility (SI). A primitive chordate (ascidian) Ciona intestinalis is an example of such an organism, but the molecular mechanism underlying its SI system is not known. Here, we show that the SI system is governed by two gene loci that act cooperatively. Each locus contains a tightly linked pair of polycystin 1-related receptor (s-Themis) and fibrinogen-like ligand (v-Themis) genes, the latter of which is located in the first intron of s-Themis but transcribed in the opposite direction. These genes may encode male- and female-side self-recognition molecules. The SI system of C. intestinalis has a similar framework to that of flowering plants but utilizing different molecules. 相似文献
44.
Asuna Aoyama Karla Cristine Callano Doysabas Atsuo Iida Eiichi Hondo 《Anatomia, histologia, embryologia》2020,49(6):681-685
The nerves that innervate the fingertips and wing membrane from the upper arm of the bent-winged bat Miniopterus fuliginosus were examined under a stereomicroscope. The radial, median, ulnar and musculocutaneous nerves were formed by the brachial plexus, which ran to the wing membrane. The two suspected axillary nerves ran to the wing membrane. The radial nerve ran to the end of the first digit, while the median nerve ran along the forearm and subsequently branched-off to run along the second to fifth digits up to the end of the phalanges. The ulnar nerve ran to the plagiopatagium on the extensor side of the elbow joint. Finally, the musculocutaneous nerve passed through the ventral side of the humerus and branched out at the elbow joint to run radially to the propatagium area. In this study, the visible nerves that were distributed from the upper arm to the fingertips of Miniopterus fuliginosus were formed by C6–T1. 相似文献
45.
Shuta Asai Miki Yoshioka Hironari Nomura Chiyori Tone Kazumi Nakajima Eiichi Nakane Noriyuki Doke Hirofumi Yoshioka 《Journal of General Plant Pathology》2011,77(3):152-162
Glucose-6-phosphate dehydrogenase (G6PDH) has been implicated in the supply of reduced nicotine amide cofactors for resistance
to biotic and abiotic stresses. Here, we show participation of the plastidic P2 isoform of G6PDH in plant immunity. A cytosolic
isoform (NbG6PDH-Cyto) and two plastidic isoforms (NbG6PDH-P1 and NbG6PDH-P2) cloned from Nicotiana benthamiana were localized in cytosol and chloroplasts, respectively. Hypersensitive response (HR) cell death and NADPH oxidase (RBOH;
respiratory burst oxidase homolog)-dependent reactive oxygen species (ROS) production after recognition of INF1 elicitin,
secreted by oomycete Phytophthora infestans, decreased in NbG6PDH-P2-silenced plants, but not in NbG6PDH-Cyto- and NbG6PDH-P1-silenced plants. Silencing of the cytosolic NAD kinase NbNADK1, which phosphorylates NADH to form NADPH, compromised HR cell death and ROS production, and concomitant silencing with NbG6PDH-P2 reduced HR cell death and ROS to levels near those in NbG6PDH-P2-silenced plants. Similarly, silencing NbG6PDH-P2 and NbNADK1 resulted in high susceptibility to P. infestans. These results suggest that NADPH produced by the P2 isoform of G6PDH in chloroplasts is responsible for HR cell death and
ROS production mediated by RBOH and that NbNADK1 is involved in this pathway. 相似文献
46.
Robert M. Boddey Phillip M. Chalk Reynaldo Victoria Eiichi Matsui 《Soil biology & biochemistry》1983,15(1):25-32
The contribution of associated biological nitrogen fixation to the nitrogen nutrition of Paspaulum notatum cv. batatais was estimated using the 15N-isotope dilution technique. The plants were grown in the field in concrete cylinders (60 cm dia) filled with soil, to which were added small quantities of 15N-labelled fertilizer at frequent intervals over 12 months. The pensacola cultivar of P. notatum was used as a non-N2-fixing control plant. This was justified by the observation that nitrogenase (intact core C2H2 reduction) activity associated with the pensacola cultivar was consistently much lower than that of the batatais cultivar.At the first harvest, no evidence for N2 fixation associated with the batatais cultivar was obtained, probably because of slow establishment of the N2-fixing association. However, at the subsequent three harvests the batatais cultivar exhibited a lower 15N-enrichment and yielded more N than the pensacola cultivar. These data together suggested that 8–25% of the N in the batatais cultivar originated from N2 fixation.The grass Paspalum maritimum was also included in the experiment and exhibited low nitrogenase activity similar to that of the pensacola cultivar of P. notatum. However, the total N and 15N data of these two grasses were not in good agreement indicating that it is important for the use of the isotope dilution technique that control plants are of very similar physiology and growth habit. 相似文献
47.
Kawakami E Kuroda D Hori T Tsutsui T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(5):561-562
The peripheral blood plasma testosterone (T) levels and superoxide dismutase (SOD) activity were measured in 5 azoospermic (AZ-) beagles. The mean values in the AZ-dogs were significantly lower than in 7 control beagles (P<0.001). Subcutaneous injections of 1 mug/kg GnRH analogue three times weekly in the AZ-dogs induced significant increases in mean T level and SOD activity (P<0.05) and improvement in spermatogenesis. Thus, spermatogenic function in the dog appears to be maintained by T and normal SOD activity in the testis. 相似文献
48.
Junko SHINDO Katsuo OKAMOTO Hiroyuki KAWASHIMA Eiichi KONOHIRA 《Soil Science and Plant Nutrition》2009,55(4):532-545
Using statistical data, we evaluated food supply and consumption in terms of nitrogen flow to clarify its effects on water quality from 1961 to 2005 in Japan. We revised a nitrogen flow model to incorporate food production, trade and consumption as well as nitrogen mineralization of crop residues and livestock manure and denitrification. Food consumption increased steadily from 1961 to the mid 1980s and has been almost stable since then. There was a notable increase in the consumption of livestock products. By 1996, consumption had risen to fivefold the 1961 level of consumption, but it has been stable since then. We concluded that the demand for food reached a maximum in the 1990s. The increasing demand for animal feed was filled by an increase in the imports of coarse cereals until the 1980s and of oil cakes until now. As the consumption of food and animal feed increased until the mid 1980s, the nitrogen load to the environment also gradually increased during this period, after which it tended to decrease. We estimated the nitrogen concentration in river water for 8 km × 8 km grid cells from 1961 to 2005 and compared these estimated values with measurements taken at more than 4000 points from public bodies of water in 1998. The spatial distribution and prefectural average of the estimated nitrogen concentration roughly corresponded with the measured values, and both indicate that food supply and consumption have had considerable effects on water quality in Japan. 相似文献
49.
Momotani E Romona NM Yoshihara K Momotani Y Hori M Ozaki H Eda S Ikegami M 《Veterinary immunology and immunopathology》2012,148(1-2):55-68
Paratuberculosis (Ptb), caused by Mycobacterium avium subsp. paratuberculosis (Map), is a chronic enteritis that affects many ruminants and other wild animals worldwide. Ptb is a great concern in animal health and in etiology of human Crohn's disease (CD). In the present study, we detected Map-specific insertion sequence IS900 of DNA in tissue sections surgically removed from lesions of patients with CD (29 samples), ulcerative colitis (UC) (17 samples), and non-inflammatory bowel disease (IBD) (20 samples). We then compared the histopathological findings of 29 CD and 17 UC cases with those of 35 cases of bovine Ptb, since few comparative pathological studies of human IBD and Ptb have been conducted. The QPCR examination indicated positive results in 13.37% of CD cases, 3.57% of UC cases, and 10% of non-IBD cases. Human CD tissues typically exhibited destructive full thickness enteritis with severe lympho-plasma infiltration and scattered additional granulomas; UC lesions exhibited much less inflammation than CD lesions. Non-IBD control samples did not exhibit pathological changes. Human CD and UC lesions were very different from Ptb lesions that are characterized by predominant granuloma formation. Immunohistochemistry for Map antigen and acid-fast staining were negative in all human IBD cases but were always positive in Ptb cases. Our present comparative study strongly suggests that we reconsider the previous hypothesis that "Map infection" causes CD, even though human intestines were considered to have been exposed to the Map antigen containing the DNA. 相似文献
50.
Kazuya Minato Yuji Konaka Iris Bremaud Shiro Suzuki Eiichi Obataya 《Journal of Wood Science》2010,56(1):41-46
The potential of muirapiranga (Brosimun sp.) as a substitute material for violin bows was estimated in terms of vibrational properties, and the influence of extractives
on the vibrational properties was examined. The loss tangent of muirapiranga was somewhat higher and the specific dynamic
Young’s modulus was rather lower than the respective values for pernambuco, which is regarded as the best material for violin
bows. Therefore, muirapiranga is a poorer bow material in terms of vibrational properties. Impregnation of crude extractives
from muirapiranga decreased the loss tangent of other wood specimens. The main compounds of the extractives were identified
as xanthyletin and luvangetin. Impregnations of isolated xanthyletin and commercially available methoxsalen, which was tested
as an analogue of luvangetin, markedly decreased the loss tangent of other wood specimens. Methoxsalen and xanthyletin differ
from conventional loss tangent-decreasing substances, namely protosappanin B and hematoxylin, in terms of water insolubility
and the absence of hydroxyl groups. From the similarity in molecular characteristics of loss tangent-decreasing substances
found so far, restriction of molecular motion due to an impregnated substance in the wood matrix is suggested as one loss
tangent-decreasing mechanism. 相似文献