Human outbreak of trichinellosis in the Mediterranean island of Sardinia, Italy

Trichinella sp. infection has been documented in both humans and animals in most Mediterranean countries, yet in the past 60 years no infections have been reported on the Mediterranean islands. We describe the first outbreak of Trichinella sp. infection to have been reported on the island of Sardinia. The outbreak occurred in two villages in 2005 and involved 11 persons who had eaten raw sausages made from the same free-ranging sow. All 11 persons developed signs and symptoms of trichinellosis and seroconverted within 48 days of consuming the infected meat. The etiological agent was Trichinella britovi. Sardinia, like all Mediterranean islands, had been considered to be Trichinella-free. The present report, together with a recent report of T. britovi infection in animals on the nearby island of Corsica (France), raises questions as to the validity of the concept of Trichinella-free areas or regions.     

Basic Community Legislation on Animal Health Conditions in Aquaculture

Veterinary Research Communications -     

Second outbreak of Trichinella pseudospiralis in Europe: clinical patterns,epidemiological investigation and identification of the etiological agent based on the western blot patterns of the patients' serum

Trichinellosis is a zoonotic disease due to the ingestion of raw or undercooked meat from animals infected with the larvae of nematodes belonging to the genus Trichinella. In January–February 2015, an outbreak of trichinellosis occurred in Genoa, Northern Italy. The epidemiological link was traced back to a dinner served at an agritourism farm on 31 December 2014, where a majority of the 52 guests had consumed the ‘beef’ steak tartare. The source of infection was not traced; however, it was noted that the amount of beef purchased officially for providing at the dinner did not correspond with that served, suggesting that meat of a different origin had been added to the beef to prepare the steak tartare. Clinical and laboratory data of 30 individuals out of the 52 (57.7%), of which four were hospitalized, were consistent with that of the case definition of trichinellosis. Western blot patterns of the sera from patients with confirmed trichinellosis were similar to the diagnostic pattern identified for the reference sera of Trichinella pseudospiralis but different from those of the control sera tested for patients infected with Trichinella spiralis and Trichinella britovi. Identification of T. pseudospiralis as the aetiological agent responsible for the outbreak of trichinellosis using an indirect tool represents an advancement in the epidemiological investigation of this zoonotic disease.     

基于扩展Kalman粒子滤波的汽车行驶状态和参数估计

汽车行驶过程中的某些参数通常需要通过实验室内较为昂贵的试验设备获得,测量成本较高,而获取车辆的行驶状态和参数对于车辆行驶过程中的控制有着重要的意义。通常情况下,需要将车辆行驶状态变量和侧偏刚度等参数进行联合估计。这些参数将会被用于车辆动力学模型来分析汽车的操纵状态。本文建立了包含定常统计特性噪声的汽车动力学模型,利用龙格-库塔方法模拟模型,引入扩展Kalman滤波技术,生成粒子滤波重要性概率密度函数,对状态和参数同时进行估计,仿真结果表明,扩展Kalman粒子滤波技术改善了标准粒子滤波算法的精度,验证了算法的有效性。     

Pedogenesis of plinthite during early Pliocene in the Mediterranean environment: Case study of a buried paleosol at Podere Renieri,central Italy

A complex buried paleosol at Podere Renieri at Montalcino (central Italy) formed during a series of continental episodes within an interval of Pliocene marine sediments from about 4.1 to 4.8 My BP. The aim of this work was to document the kind of pedogenesis which occurred throughout this time and, in particular, the plinthite formation. Plinthite (soft and hard) is poor in organic carbon, neutral or subalkaline, dominated by illite, and enriched in iron and chromium. The main differences between soft and hard plinthite are attributed to soil structure, which is absent in hard plinthite, and to the abundance of iron depleted zones, which are much larger in soft plinthite. Variations in mineralogy, chemistry and genesis of soft and hard plinthite can be ascribed to the specific environment of plinthite formation, close to the Pliocene sea. Pedogenetic evidence indicates that plinthite evolved in a hot and humid paleoclimate, showing a progressive increase in seasonality, and that low-grade plinthite formation occurred in a time span of a few hundred thousand years. Soils with low-grade plinthite have agronomic value in their suitability for the production of high quality Brunello di Montalcino wine.     

From Slope Morphometry to Morphogenetic Processes: An Integrated Approach of Field Survey,Geographic Information System Morphometric Analysis and Statistics in Italian Badlands

Calanchi (singular: calanco) represent a typical example of badlands in the Italian peninsula, which rapidly evolve on clayey terrains such as the widespread Pliocene–Pleistocene marine clays. The present study aimed at investigating the role of the slope morphometry on the typology and distribution of morphogenetic processes in a calanchi area located in southern Italy. The research included detailed geomorphological surveying as well as morphometric and statistical analyses. The study area was first subdivided into individual hydrographic units (HUs), for which field survey allowed to identify the dominant denudation processes, their intensity and the distribution of the associated landforms (pipes, rills, gullies and landslides). The morphometry of each HU was characterised by calculating the morphometric slope index (MSI) on a reconstructed digital elevation model. By statistically comparing the morphogenetic and morphometric data, the influence of the slope morphometry on the type, distribution and evolution of the calanchi erosion process has been highlighted. In particular, through a cluster analysis, the groups of HUs having similar dominant processes were identified, and by applying the analysis of variance, the effect of the MSI on the identified clusters was analysed. Two clusters were identified, which revealed the relative importance of morphogenetic processes and the strict connection between surface and subsurface landforms. These two clusters were discriminated by the MSI value (high MSI favoured mass movements, while low MSI favoured gully erosion and piping), highlighting the importance of slope morphometry in driving the morphogenetic hillslope processes. Copyright © 2015 John Wiley & Sons, Ltd.     

Soil water availability in rainfed cultivation affects more than cultivar some nutraceutical components and the sensory profile of virgin olive oil

This research considered the varieties 'Frantoio' and 'Moraiolo' growing in rainfed olive trees (Olea europaea) and took place in Tuscany, central Italy. Soil moisture was monitored during the very meteorologically contrasting years 2002 and 2003 in two nearby olive groves. The plots had the same morphological and climatic conditions, but different soil types. Monocultivar oil samples were analyzed to determine fatty acids, minor polar compounds, and tocopherols content and were submitted to organoleptic analysis by a panel of trained tasters. The results highlighted that soil water regimen affects some nutraceutical components and the sensory evaluation of olive oil. Cultivar also affected yield components, polyphenols, and tocopherols content, but less than soil water availability. The plants on the soil inducing a relatively more intense and longer water deficit during summer (a Skeleti Calcaric Regosol) had an early ripening and gave the best results in terms of phenolic compounds and, consequently, antioxidant properties of the olive oil. The sensorial properties of the oil obtained from both cultivars on the Regosol were superior in both years of the trial.     

Inappropriate feeding practice favors the transmission of Trichinella papuae from wild pigs to saltwater crocodiles in Papua New Guinea

The recent discovery of Trichinella zimbabwensis in farmed crocodiles (Crocodilus niloticus) of Zimbabwe and its ability to infect mammals, and the development of both T. zimbabwensis and Trichinella papuae in experimentally infected reptiles led to an investigation of Trichinella infection in saltwater crocodiles (Crocodylus porosus) and in wild pigs (Sus scrofa) of Papua New Guinea, to see if T. papuae also, is present in both cold- and warm-blooded animals. Of 222 crocodiles examined, 47 animals (21.2%), all from Kikori, Gulf Province, were positive for non-encapsulated larvae in the muscles. The greatest number of larvae was found usually in the biceps, with an average of 7 larvae/g. One isolate from a crocodile infected successfully both laboratory rats and mice. Of 81 wild pigs examined, 9 from Bensbach river area (Western Province) and 1 from Kikori area (Gulf Province) were positive for non-encapsulated larvae in the muscles. Trichinella larvae from both saltwater crocodiles and wild pigs have been identified by multiplex-PCR analysis as T. papuae. The sequence analysis of the region within the large subunit ribosomal DNA, known as the expansion segment V, has shown the presence of a molecular marker distinguishing T. papuae isolates of Bensbach river area from those of Kikori area. This marker could be useful to trace back the geographical origin of the infected animal. The epidemiological investigation carried out in the Kikori area has shown that local people catch young crocodiles in the wild and keep them in holding pens for several months, before sending them to the crocodile farm in Lae (Morobe Province). They feed the crocodiles primarily with wild pig meat bought at the local market and also with fish. These results stress the importance of using artificial digestion for routinely screening of swine and crocodiles, and of adopting measures for preventing the spread of infection, such as the proper disposal of carcasses and the adequate freezing of meat.     

Genetic analysis of Trichinella populations by 'cold' single-strand conformation polymorphism analysis

A non-isotopic single-strand conformation polymorphism ('cold' SSCP) technique has been assessed for the analysis of sequence variability in the expansion segment 5 (ES5) of domain IV and the D3 domain of nuclear ribosomal DNA within and/or among isolates and individual muscle (first-stage) larvae representing all currently recognized species/genotypes of Trichinella. Data are consistent with the ability of cold SSCP to identify intra-specific as well as inter-specific variability among Trichinella genotypes. The cold SSCP approach should be applicable to a range of other genetic markers for comparative studies of Trichinella populations globally.     

Cystic Echinococcosis in Water Buffaloes from the Campania Region of Southern Italy

Veterinary Research Communications -