不同氮水平对黄瓜蚜虫生长发育的影响

通过不同氮水平处理,研究瓜蚜Aphis gossypii生殖力、黄瓜叶片游离氨基酸、可溶性糖含量的变化,探讨施肥水平与蚜虫繁殖的关系。结果显示,高氮水平(210、280mg/L)处理瓜蚜生殖力明显高于低氮水平(70、140mg/L)处理,其中140 mg/L氮水平处理,蚜虫的内禀增长率、单雌平均每日产仔量、净增值率等最低,种群数量增加量最小;不同氮水平处理黄瓜叶片内游离氨基酸和可溶性糖含量有明显变化,随着氮水平增加,16种游离氨基酸的总量增加、可溶性糖的含量降低;蚜虫内禀增长率与叶片内游离氨基酸增加量和对照增加量的比值呈现正相关,与可溶性糖的含量呈负相关,且均达到显著水平。结果表明不同氮水平通过改变叶片内游离氨基酸和可溶性糖的含量而影响蚜虫的繁殖和种群的增长。     

陕西关中东部棉花抗旱品种农艺节水综合技术研究

按照生态农业理论和抗旱节水技术要求,对棉花抗旱品种、不同密度、不同基肥N、P的施用量进行田间综合试验,采用正交设计法。3年试验研究结果表明:密度以每公顷种植7.5万株比4.5万株、6万株平均增产671.85kg、483.30kg,增产57.49%和41.36%。品种陕402(早熟)、陕576(中早熟)分别比中棉所19(中熟)增产16.62%和16.17%,具有较强抗旱性。磷酸二铵比尿素增产作用明显,每公顷施150、225kg分别增产11.35%和10.53%。     

Oligochitosan induces cell death and hydrogen peroxide accumulation in tobacco suspension cells

Oligochitosan has been shown to induce several plant defense responses. In the present work, the effect of oligochitosan on tobacco cell survival was investigated. The results showed that oligochitosan caused tobacco cell death in a dose-dependent manner. About 40.6 % tobacco cells died when cultured for 72 h after 500 μg ml⁻¹ oligochitosan treatment. Certain aspects of this cell death process appeared to be similar to apoptosis in animal cells. These included shrinkage of cytoplasm and condensation of chromatin. Oligochitosan also induced H₂O₂ accumulation in tobacco cell suspension culture. The role of H₂O₂ in the signal transduction that leads to cell death was investigated. Co-treatment of tobacco cells with oligochitosan and catalase inhibited H₂O₂ accumulation but did not inhibit the induction of cell death. The results suggested that apoptosis-like cell death of tobacco cells induced by oligochitosan is independent of H₂O₂ signal pathway.     

用大肠杆菌K99和F41菌毛抗原检测牛血清抗体的琼脂扩散试验的研究

用含２ｍｏｌ／Ｌ尿素的磷酸盐缓冲液以热处理方法提取的Ｋ９９和Ｆ４１菌毛作为抗原,对琼脂扩散试验方法进行研究。结果表明本试验方法简便,特异性强,敏感性高,可用于血清抗体检测;所制备的抗原稳定、重复性好。     

家兔发情状态与受胎率和产仔数关系的研究

本文研究了家兔的发情状态与配种后的受胎率，以及产仔数之间的关系。以家兔阴粘膜颜色作为表观指标，将家兔的发情分为５个状态，其相应的阴粘膜颜色分别为：苍白、粉色、红色、大红、黑紫。经促排２号诱发排卵后，人工授精，相应状态下的受胎率为：２９．５％、４２．９％、５６．８％、５３．８％、２５．０％；相应状态下的平均产仔数分别为：５．５４、６．６９、６．６２、７．２１、５．５２。用促排３号处理后，相应状态下的受胎率为：９．０％、４３．２％、５３．２％、７０．０％、２１．７％。结果表明：无论是用促排２号，还是用促排３号处理，当阴粘膜颜色为苍白和黑紫时的受胎率或产仔数，都显著地低于或少于阴粘膜颜色为粉色、红色、大红时的受胎率（Ｐ＜０．０１）或产仔数（Ｐ＜０．０５）。说明，家兔的发情状态与配种后的受胎率和产仔数有密切的相关     

The pathology of heartwater. I. A study of mice infected with the Welgevonden strain of Cowdria ruminantium

Gross and microscopical lesions in mice intravenously infected with the Welgevonden strain of Cowdria ruminantium closely resembled the lesions described in cattle, sheep and goats. A high concentration of organisms was present in alveolar endothelial cells. Cytopathic changes in parasitized and non-parasitized endothelial cells and the morphology of the organisms are described and compared with the Ball3 strain of C. ruminantium. Possible mechanisms in the development of the lung oedema are considered and the role of mice as animal model is discussed.     

6例奶牛子宫扭转的诊断与治疗

奶牛子宫扭转是发生于妊娠期的一种常见的难产性疾病,通常妊娠子宫沿自身纵轴发生一定角度的扭转。轻度的子宫扭转(≤90°)多发生于妊娠早期和中期,多可自行恢复;但严重的子宫扭转多发生于妊娠后期,通     

活性炭负载磷钨酸催化α-蒎烯的异构化反应

研究了磷钨酸、活性炭负载磷钨酸催化α－蒎烯的异构化反应，考察了反应条件对α－蒎烯转化率和产物选择性的影响。结果表明：活性炭负载磷钨酸催化α－蒎烯的异构化反应具有反应温度低、催化活性高、莰烯和荣烯选择性高等优点，将磷钨酸负载于活性炭载体上以后，其催化活性和稳定性增大，并可重复使用。