GIS在窟野河流域沙棘减沙工程监测中的应用

GIS地理信息系统是集数据采集、处理分析、管理查询和输出等功能为一体的现代技术工具,GIS正在被广泛应用在水土保持监测领域,本文阐述GIS技术在窟野河流域沙棘减沙工程监测中的应用。     

甘南州林区的大型药用真菌

本文报道了在甘南林区的大型药用真菌１６５种。并对其重要种类进行列述,提出了合理开发利用的措施和建议。     

根癌农杆菌介导豇豆胰蛋白酶抑制剂基因转入苹果主栽品种

研究建立起一套简单、高效的苹果遗传转化系统。利用根癌农杆菌介导的叶圆片转化法将高效启动的豇豆胰蛋白抑制剂基因（ＣｐＴｌ）转入富士、齐纳金、王林、嘎拉等苹果主栽品种中,经卡那霉素抗性、ＰＣＲ、点杂交、Ｓｏｕｔｈｅｍ杂交检测证明了４个品种均获得转化再生植株,现已移栽成活。     

黄河流域棉区北部机采棉田恶性杂草的化学防除研究

影响黄河流域棉区北部棉花机械采收的恶性杂草主要有藜、反枝苋、铁苋菜、龙葵、苘麻、田旋花、打碗花和裂叶牵牛等。于2013、2014、2016年研究了这些杂草的化学防除方法。苗蕾期用嘧硫草醚和三氟啶磺隆对藜、反枝苋、龙葵、苘麻、田旋花、打碗花的防效较好,药后30～40 d的株防效超过95%;对铁苋菜和鹅绒藤的防效较差,同期株防效不足85%;对裂叶牵牛的防效居中,同期株防效为84%～94%。花铃期用嘧硫草醚和三氟啶磺隆对上述恶性杂草的防效下降;而乙氧氟草醚的杀草活性较强,药后25～40 d株防效可达100%（裂叶牵牛除外）。草甘膦在苗蕾期、花铃期和吐絮期对各种恶性杂草的株防效均在95%～100%（花铃期的裂叶牵牛除外）。上述处理对棉花均未产生明显药害,对产量也无显著影响。针对黄河流域棉区机采棉田,苗蕾期应用嘧硫草醚或/和三氟啶磺隆,花铃期应用乙氧氟草醚或草甘膦,吐絮期应用草甘膦,可较好地防除恶性杂草。     

Numerical analysis of injection molding for the Syringe Barrel with optimum design and processing condition

The numerical analysis was performed to predict the potential problem, often occurring during the manufacturing process of the disposable medical device of a great volume. The cavity filling analyses were performed for the new design of the 3cc Syringe Barrel using polypropylene(PP1), and a new nucleated polypropylene(PP2) material for better clarity. These analyses have been performed for different processing conditions as well as various wall thickness designs for both materials. With the nucleated material, only the original wall thickness design has been studied at two different processing conditions for comparison purpose. This study was conducted to investigate the optimum part design and processing condition for two different materials. The most desirable design was selected with Design 3 for material utilization and reduced flow stresses by comparing the field results. The new nucleated polypropylene provided slightly better product quality and processing.     

Gene cloning of ZmMYB59 transcription factor in maize and its expression during seed germination in response to deep‐sowing and exogenous hormones

Discovering the key genes responsible for deep‐sowing germination is very meaningful to cultivate maize varieties with strong germination ability in soil. In this study, ZmMYB59 gene whose functions remain unknown was successfully cloned. Subcellular localization showed that ZmMYB59 was localized in the nucleus. By analysing cis‐acting elements of its promoter sequence, many elements of MYB binding domain responsive to hormones (particularly gibberellin, GA) were found. On this basis, ZmMYB59 expression in different sowing depths, germination stages, tissues and treatments was analysed by real‐time PCR. ZmMYB59 expression levels in embryos or seedlings were significantly decreased with the increase in sowing depth at 2 days or 4 days after sowing (DAS). Further analysis showed that its expression in mesocotyls was also significantly downregulated with the increase in sowing depth at both 6 DAS and 8 DAS. Similarly, seed soaking with 10^‐5 M GA₃ inhibited ZmMYB59 expression in mesocotyls at 3 DAS. These results suggest that ZmMYB59 may play a negative regulatory role in seed germination in deep soil and the regulation is involved in GA signalling pathway.     

6种杀菌剂对西瓜炭疽病的防效试验

采用6种杀菌剂对西瓜炭疽病进行田间药效试验。3次药后试验结果表明:50%咪鲜胺锰盐可湿性粉剂和25%吡唑醚菌酯乳油效果最好,防效在73%以上;10%苯醚甲环唑水分散粒剂和25%溴菌腈可湿性粉剂防效次之,防效在67%以上;50%多菌灵可湿性粉剂和80%代森锰锌可湿性粉剂防效较差,防效为50%左右。药剂处理后,50%咪鲜胺锰盐可湿性粉剂、25%吡唑醚菌酯乳油和对照,中心可溶性固形物含量分别为11.8%、12.0%、9.8%;两种药剂比对照分别增产30.96%、28.26%,增产效果显著。因此,推荐50%咪鲜胺锰盐可湿性粉剂和25%吡唑醚菌酯乳油为目前防治西瓜炭疽病的理想药剂。     

吲哚丁酸对葡萄不同鲜食品种硬枝扦插成活率的影响

以京秀、早黑宝、白鸡心、克伦生4个鲜食葡萄品种的1年生枝为试材,通过对生根促进剂吲哚丁酸浓度的筛选试验,选择吲哚丁酸(2 000mg/L)对葡萄硬枝进行扦插成活率的影响试验。结果表明:克伦生、白鸡心生根情况较好,早黑宝居中,京秀最差。     

棉花种质资源收集鉴定与创新利用

十二五期间通过对我国西南地区、南海诸岛、及国外野生棉起源中心实地考察和广泛征集,新收集国内外棉花种质资源1220份,其中陆地棉986份、海岛棉176份、亚洲棉58份,所收集种质均已入国家中期库保存。收集的棉花种质资源分别在河南安阳、海南三亚、新疆阿拉尔进行主要农艺经济性状鉴定和特性鉴定。通过SSR(Simple sequence repeat)分子标记、重测序等技术对收集到的种质进行了分子鉴定,筛选到大量与纤维品质、抗黄萎病、抗旱、抗草甘膦显著关联的基因。在收集保存的种质资源基础上,通过辐射诱变、远缘杂交、复合杂交创造出优质、高产、抗逆等优异种质资源。通过展示向全国科研院所及公司发放棉花种质19 191份次。