Progesterone supplementation during multiple ovulation treatment in buffalo species (<Emphasis Type="Italic">Bubalus bubalis</Emphasis>)

The aim of this study was to evaluate the effect of exogenous progesterone supplementation on superovulatory response in buffaloes that has undergone a multiple ovulation program. Fourteen Mediterranean buffaloes were divided into two groups and received a 4-day decreasing dosage of an equal mixture of 500 IU of FSH and LH starting on day 8 of the cycle. In group A (n = 7) a progesterone-releasing intravaginal device was removed on day 8, whereas in group B (n = 7) it was left till day 10, when PGF_2a {\hbox{PG}}{{\hbox{F}}_{{2}\alpha }} was administered. Eighty hours later, buffaloes were artificially inseminated and after 6 days they undergone uterine flushing. A higher (P < 0.05) number of corpora lutea (8.3 vs. 5.7) and embryo/flushing/buffalo (2.3 vs. 1.3) were recorded in group B vs. group A if responsive buffaloes are considered (n = 12) and the number of corpora lutea was highly correlated with the number of embryos (r = 0.65; P < 0.05). In conclusion, progesterone supplementation during the first 2 days of the superovulation treatment seems to enhance the recovery rate in buffalo species. A high ovulation rate, associated with a high number of corpora lutea, can represent a parameter for estimating embryo recovery.     

Anthrax undervalued zoonosis

Anthrax is a non-contagious disease, known since ancient times. However, it became a matter of global public interest after the bioterrorist attacks in the U.S.A. during the autumn of 2001. The concern of politicians and civil authorities everywhere towards this emergency necessitated a significant research effort and the prevention of new bioterrorist acts. Anthrax is primarily a disease that affects livestock and wildlife; its distribution is worldwide; and it can represent a danger to humans but especially more so when it occurs in areas considered to be free and in atypical seasons and climatic conditions. The atypicality of the phenomenon may lead health workers to misdiagnose and, consequently, an inappropriately manage of affected carcasses with a consequent and inevitable increase in the risk of human infection. This article emphasises the importance of paying increasing attention to this zoonosis. The biggest risk is its underestimation.     

Characterization of the lentil landrace Santo Stefano di Sessanio from Abruzzo,Italy

In the world lentil is grown on more than 3 million hectares and is one of the most important, low-cost, food source of protein. In Italy lentil has been cultivated since ancient times, but in the last decades its cultivation has been confined to marginal areas, small islands and hilly, mountainous areas of central and southern Italy. Local varieties are still common and are often greatly appreciated for their taste and cooking qualities. Several accessions from the Santo Stefano di Sessanio area, Abruzzo Region, were collected and phenotypically and genotypically characterized in order to look for the existing variability within and between populations. Image analysis of seeds was also used. Populations grown in Santo Stefano di Sessanio and in the neighbouring area basically share most of their characteristics. However, some of the accessions anonymously gathered from the local market were shown to be different from those collected from farmers. The paper reports and discusses how this local product needs be characterized and promoted in order to avoid fraud that could negatively affect the local economy and put valuable, adapted, genetic resources at risk of erosion.     

On the relations between critical behaviour of soil moisture spatio-temporal dynamics and catchment descriptors

When changing from spatially random to spatially connected appearances as conditions become wetter in their seasonal time dynamics, soil moisture spatial patterns show a behaviour similar to phase transition processes. In a previous work, once implemented an ad-hoc methodology, we have demonstrated that such a phase change shows critical point behaviour. Moreover, the value of the critical probability seems to be an intrinsic characteristic of a catchment, being insensitive to renormalization (rescaling), as well as to model parameterization.     

Malignant melanoma in a seagull (Larus fuscus): morphological and immunohistochemical approach

A common seagull (Larus fuscus) was found near the southern coast of Italy by the veterinarians of the local wild animal rescue center. Physical examination of the bird revealed an ulcerated mass involving a majority of the oral cavity; the mass did not allow for normal feeding. After the bird died necropsy was performed and the mass was histologically and immunohistochemically examined. The morphology and the immunoreactivity for Melan-A and S-100 antigens led to a diagnosis of malignant melanoma. This is the first case of malignant melanoma described in a seagull, and herein we compare the characteristics of the present case with malignant melanoma already described in domestic animals.     

Prevalence and genetic characterization of Giardia and Cryptosporidium in cats from Italy

One hundred and eighty one cats living in central Italy were tested for the presence of Giardia and Cryptosporidium infection by IFAT test and specific PCRs. Overall eight (4.4%) samples were IFAT-positive for Giardia. All the IFAT-positive samples for Giardia scored positive for the PCRs, and three more samples IFAT-negative generated PCR products leading to a total 6.1% molecular positivity rate for Giardia. All the examined samples were negative for Cryptosporidium. Sequencing of samples molecularly positive to Giardia indicated that three cats harbored the zoonotic Giardia duodenalis Assemblage A, whereas all other positive animals were infected with the feline-specific G. duodenalis Assemblage F. Phylogenetic analysis carried out on the sequences obtained supported the clustering of the isolates within Assemblages A and F. The results here presented provide data on the occurrence of Giardia genotypes in cats living in close contact with humans highlighting the potential importance of this protozoan disease for the public health.     

Traumatic myiasis by Wohlfahrtia magnifica in Italy

Seven farms, two in Central Italy and five in Southern Italy, were visited for the presence of larvae-infested wounds. Ninety-six (3%) out of 3129 sheep examined had a traumatic myiasis with a very high percentage of infested animals found only in a farm (10.5%). Wounds were mainly localized on the vulva and prepuce. Infested animals were restless, anxious, and reluctant to graze. None of the 10 goats in one infested flock had wounds, whereas a shepherd dog from another flock presented a wound on the ear. All the larvae and adults reared from them in the laboratory were identified as Wohlfahrtia magnifica (Schiner, 1862) (Diptera, Sarcophagidae). The cases presented here are the first clinical cases of wohlfahrtiosis in sheep and dogs reported in Italy, and indicate that this myiasis is endemic in this country. A wider epidemiological investigation on the prevalence, incidence and seasonality of W. magnifica infestation of Italian W. magnifica population/s would be useful to understand its geographical provenience and the likely risk for its spreading throughout Italy and other non-endemic areas.     

PREVIOUS CROPS AND ORGANIC FERTILIZERS IN LETTUCE: EFFECTS ON YIELDS AND SOIL PROPERTIES

The use of organic amendments in agriculture could sustain crop production and preserve the agroecosystem, due to their importance in the conservation of organic matter in soil. The objective of this research was to evaluate the effects of differing organic fertilizers and of previous crops on lettuce growth, nutritional status and yields. The effects on soil characteristics were further investigated. The research was carried out during two years (2006 and 2007) at Metaponto (MT) in southern Italy, on lettuce crops cultivated after eggplant and melon to avoid their continuous cropping. The effects of application of a mineral fertilizer (MF), and of three treatments with organic fertilizers (commercial stable manure - OM; anaerobic digestate based on wine distillery wastewater - AD; composted municipal solid organic wastes coming from the separate collection - MSW) were studied. Head average weight, leaf area index, nitrate content and SPAD readings during the cropping cycles did not show significant differences among fertilizer treatments. On the contrary, marketable yield and head average weight at the harvest presented significantly different among the three organic fertilizers. The average marketable yield and head average weight of organic fertilizers experimental (AD and MSW treatments) decreased of 16 and 17%, respectively, compared to OM treatment. The previous melon crop influenced positively all analyzed parameters of the lettuce compared with previous eggplant crop. The previous melon crop reached the highest marketable yield with an increase of 59% compared with previous eggplant crop. Compared to the mineral fertilizer, the organic ones significantly increased the extracted fraction of soil organic carbon (6.9, 10.7 and 14.9% for OM, AD and MSW, respectively), without significant changes for the humic and fulvic content.