Effects of epidural injections and transvaginal aspiration of ovarian follicles in heifers used repeatedly for ultrasound-guided retrieval of ova and embryo production

Postmortem examinations of 13 Simmental heifers that had received between 16 and 28 injections to induce caudal epidural anaesthesia, the last not less than seven months before they were slaughtered, showed that none of them had any evidence of infection or inflammation at the injection site or in adjacent bone and soft tissues. Seven of them had minor damage to intercoccygeal discs, consisting of discospondylosis with neovascularisation and chondroid metaplasia, consistent with injuries caused by needles. The severity of the damage was not related to the number of epidural injections received, suggesting that the damage was probably caused by a discrete suboptimal injection procedure. In a second study, the ovaries from 22 Simmental heifers that had undergone between 13 and 16 transvaginal follicular aspirations were examined postmortem. Approximately one-third of them had a natural texture with little or no evidence of scar tissue, and less than one in five had extensive scarring and a toughened texture. There was no evidence of compromised ovarian function, as determined by the number and normality of corpora lutea and large follicles, in any of the animals.     

Selected Elastic Moduli of Single-Crystal Olivines from Ultrasonic Experiments to Mantle Pressures

Ultrasonic interferometric measurements, developed for polycrystalline samples in a multi-anvil apparatus, were extended to single-crystal samples of San Carlos olivine and forsterite. The elastic moduli, C22 and C55 of San Carlos olivine and C55 of pure forsterite, were measured to about 13 gigapascals. These data on C22 for San Carlos olivine and C55 for forsterite are consistent with earlier measurements and extrapolations. The C55 for San Carlos olivine increases linearly as a function of increasing pressure, unlike the earlier nonlinear behavior observed at high pressure with impulsive stimulated scattering techniques.     

Thermal Structure of Jupiter's Upper Atmosphere Derived from the Galileo Probe

Temperatures in Jupiter's atmosphere derived from Galileo Probe deceleration data increase from 109 kelvin at the 175-millibar level to 900 ± 40 kelvin at 1 nanobar, consistent with Voyager remote sensing data. Wavelike oscillations are present at all levels. Vertical wavelengths are 10 to 25 kilometers in the deep isothermal layer, which extends from 12 to 0.003 millibars. Above the 0.003-millibar level, only 90- to 270- kilometer vertical wavelengths survive, suggesting dissipation of wave energy as the probable source of upper atmosphere heating.     

Grb14 mRNA Levels During Follicular Deviation in Cattle are Higher in Granulosa Cells of Subordinate Compared to Dominant Follicles

The growth factor receptor‐bound protein 14 (Grb14) is a cellular adapter protein belonging to the Grb7 family of proteins. Studies with human and rodent cells have demonstrated that Grb14 acts as a negative regulator of tyrosine kinase receptor signalling through the MAPK and PI3K pathways. In cattle, tyrosine kinase receptors are activated during follicular development but the role of Grb14 in this process has not yet been investigated. Therefore, the aim of the present study was to characterize Grb14 mRNA expression in ovarian somatic cells during follicular growth and deviation in cattle. We found Grb14 mRNA expressed in both granulosa and theca cells derived from follicles at different stages of development (3–5 , 6–8, >8 mm in diameter). The abundance of mRNA for Grb14 was higher in granulosa cells of subordinate compared with those from dominant follicles at days 3 and 4 of the follicular wave (p < 0.05). Further, there was a negative correlation between the abundance of mRNA for Grb14 and P450Arom in granulosa cells (R² = 0.367; p < 0.05) and between the abundance of mRNA for Grb14 in granulosa cells and concentration of oestradiol in follicular fluid (R² = 0.545; p < 0.05). In theca cells, the expression of Grb14 mRNA did not differ between dominant and subordinate follicles (p > 0.05). These findings suggest that Grb14 may play a regulatory role in granulosa cells during follicular deviation in cattle.     

A comparison of random draw and locally neutral models for the avifauna of an English woodland

Background

Extensive work has been done to identify and explain multi-year cycles in animal populations. Several attempts have been made to relate these to climatic cycles. We use advanced time series analysis methods to attribute cyclicities in several North-American mammal species to abiotic vs. biotic factors.

Results

We study eleven century-long time series of fur-counts and three climatic records – the North Atlantic Oscillation (NAO), the El-Niño-Southern Oscillation (ENSO), and Northern Hemisphere (NH) temperatures – that extend over the same time interval. Several complementary methods of spectral analysis are applied to these 14 times series, singly or jointly. These spectral analyses were applied to the leading principal components (PCs) of the data sets. The use of both PC analysis and spectral analysis helps distinguish external from intrinsic factors that influence the dynamics of the mammal populations.

Conclusions

Our results show that all three climatic indices influence the animal-population dynamics: they explain a substantial part of the variance in the fur-counts and share characteristic periods with the fur-count data set. In addition to the climate-related periods, the fur-count time series also contain a significant 3-year period that is, in all likelihood, caused by biological interactions.