用Nested-PCR检测广西黄皮与九里香黄龙病病原

摘要：运用nested-PCR技术对广西黄皮和九里香进行柑桔黄龙病（Citrus Huanglongbing, HLB）检测,结果表明：从部分黄皮和九里香样品抽提的DNA中,可扩增出一条特异性的400 bp条带,说明广西种植的黄皮和九里香均可感染黄龙病;在柑桔黄龙病疫区,黄皮和九里香黄龙病感染率分别为35.0%和40.0%。     

车载式拖拉机座椅舒适性测试系统的研究

应用美国模拟器件公司的ADXL105微型力平衡式加速度传感器、美国Cygnal公司F320型单片机组成车载式拖拉机座椅舒适性测试系统，系统易标定、成本低、供电方式简单。试验结果表明，在其他条件相同时，拖拉机座椅固有频率只要避开人体敏感的频率范围，座椅的舒适性就能得到明显的改善。     

论节能式炉气体木材干燥室的设计

本文讨论了节能式炉气干燥室设计的一些技术问题，如提高自生风引力增强通风；多种传热方式并用，增强传热，降低热损失的途径等，以达到节能降耗、快速干燥的目的。     

红枣气体射流冲击干燥收缩特性研究

Chinese jujube originated in China and has a long historical standing. China is the only country which can produce a great amount of Chinese jujube in the world. Modern research showed that the nutrition of Chinese jujube is abundant. Chinese jujube has great medicinal and food value, with high economic value and good prospects for development. Shrinkage characteristics of dates generally refer to volume shrinkage characteristics of whole dates. Large fruit and plump grain appearance of dates is the first choice of consumers to buy elements. The volume of dried jujube points to the consumer’s heart, and is an important indicator of consumers whether to buy. Jujube processing enterprises grade the date in accordance with its size, different grades dates have different value and price. Therefore, drying is the most important steps during the dates processing as it affects directly the quality of the product, and the shrinkage characteristics is the most important quality characteristic of Chinese jujube after drying. However, the traditional natural desiccation and desiccation in the drying room of the red dates has many problems. The conventional drying method for Chinese jujube has some disadvantages of severe shrinkage, long drying time and large energy consumption etc. Therefore, in order to improve the quality of dried Chinese jujube and reduce the shrinkage, it is imperative to replace the traditional drying method with the modern technologies. Air impingement drying technology was applied to dry Chinese jujube. The drying characteristics of shrinkage, moisture effective diffusivity considering shrinkage characteristics and the activation energy of Chinese jujube shrinkage were investigated under different air temperatures (55, 60, 65 and 70℃) and air velocities (6, 9, 12 and 15m/s). The dynamic changes of the regular pattern for the shrinkage characteristic of Chinese jujube in the air impingement drying process were studied, the results showed that the volume relative shrinkage ratio of Chinese jujube appeared to increase and then decrease with the increase of air temperature and air velocity. Considering shrinkage characteristics, the moisture effective diffusivity of Chinese jujube in the air impingement drying process through the Fick’s second law was obtained. The diffusivity appeared to increase and then decrease with the decrease in moisture content in dry basis of Chinese jujube. The Arrhenius equation calculated the shrinkage activation energy of the Chinese jujube was 1281kJ/mol. This research provided technical basis for shrinkage characteristics of Chinese jujube using air impingement drying technology.     

杭州市森检对象和补充森检对象调查

杭州市森林面积９６２６６７ｈｍ＾２，种苗花卉木材及林产品的调运十分频繁，经１９９５￣１９９７年调查，分布国内森检对象８种、外省补充森检对象８种，根据发生、危害情况分类施策，提出控制扑灭等措施。     

暖通CAD信息流分析和公用数据库的研究

分析了暖通ＣＡＤ信息流的特点，提出了交互式，集成化，智能化暖通ＣＡＤ的开发构想，同时从暖通设计过程的信息流入手，开发了基于图形用户界面思想的公用数据库及其管理繁育 及基于谐波反应法的负荷计算软件。     

大面积用材林代化栽培模式技术

林业生产要达到当今经济技术条件下最大限度的速生、丰产、优质、高效稳定和持续，不仅要有良种壮苗，还要做好造林地调查与规划，做到适地适树、适时适法，这是实现林业优化栽培模式的前提与保证；同时，造林树种选择、造林时间、密度确定、种植点配置、栽培方法、林木施肥、营造混交林以及森林保护、造林地设保护带、造林地留栽补植苗，这一系列均是用材林优化栽培模式的重要技术措施。     

苹果酸处理水稻种子对秧苗根系及抗寒性影响的初探

水稻种子经苹果酸处理后,淀粉酶过氧化氢酶和脱氢酶活性均有提高。其幼苗根系发达,根/茎叶比重增加。幼苗经5C冷处理后,超氧化物歧化酶活性和叶绿素含量虽表现出下降,但明显高于对照。丙二醛含量和电解质渗漏率比对照为低。苹果酸处理在一定程度上提高了水稻秧苗素质及抗寒能力。     

Exploring genetic diversity of rice cultivars for the presence of brown planthopper (BPH) resistance genes and development of SNP marker for Bph18

Brown planthopper (BPH) is the most devastating insect pest in rice‐growing areas. Information on availability of BPH resistance alleles and their sources enhances BPH‐resistant breeding programmes. In this study, 260 highly diversified rice cultivars or breeding lines were screened for the presence of five major BPH resistance genes (Bph10, Bph13, Bph18, Bph20 and Bph21) using gene‐specific markers. The analysis revealed that 137 of the 260 cultivars possess at least one BPH resistance gene. Bph10 was predominant while Bph20 was the least distributed. Moreover, two and three different resistance gene combinations were found in the cultivars. Molecular markers play an important role in molecular breeding programmes. A tightly linked PCR‐based co‐dominant Bph18 marker was developed, which is cost effective and time effective and simpler than available Bph18 CAPS marker (7312.T4A). We strongly believe that the identified BPH‐resistant cultivars can be used as alternative resistance gene sources and also as resource for novel BPH resistance genes. The developed Bph18 marker will be highly useful in molecular breeding applications of BPH‐resistant breeding programmes.     

浅圆仓不同功率风机通风试验研究

利用2.2kW的轴流风机组与7.5kW的离心风机组,对两个浅圆仓进行通风降温,经对比试验,在储粮数量、储粮品种、入仓时间、入仓质量、粮情状况等基本相同的浅圆仓中,当降温效果基本相同时,2.2kW的轴流风机组通风时间较长,7.5kW离心风机组降温效果较快,但水损较多,2.2kW的轴流风机组通风耗能较低,吨粮降温成本较低。