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21.
Cardiomyopathy developed in mice deficient for α-kinase 3 (ALPK3), a nuclear kinase previously implicated in the differentiation of cardiomyocytes. Alpk3 (-/-) mice were produced according to normal Mendelian ratios and appeared normal except for a nonprogressive cardiomyopathy that had features of both hypertrophic and dilated forms of cardiomyopathy. Cardiac hypertrophy in Alpk3 (-/-) mice was characterized by increased thickness of both left and right ventricular (LV and RV) walls and by markedly increased heart weight and increased heart weight/body weight and heart weight/tibia length ratios. Magnetic resonance imaging studies confirmed the increased thickness in both septal and LV free walls at end-diastole, although there was no significant change in LV wall thickness at end-systole. Myocardial hypertrophy was the predominant feature in Alpk3 (-/-) mice, but several changes more typically associated with dilated cardiomyopathy included a marked increase in end-diastolic and end-systolic LV volume, as well as reduced cardiac output, stroke volume, and ejection fractions, suggesting LV chamber dilation. Magnetic resonance imaging showed a 50% reduction in both septal and free wall LV contractility in Alpk3 (-/-) mice. Interstitial fibrosis and inflammation were notably absent in Alpk3 (-/-) mice; however, light and electron microscopy revealed altered cardiomyocyte architecture, characterized by reduced numbers of abnormal intercalated discs being associated with mild disarray of myofibrils. These lesions could account for the impaired contractility of the myofibrillar apparatus and contribute to the pathogenesis of cardiomyopathy in Alpk3 (-/-) mice. 相似文献
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屠宰企业的非洲猪瘟自检对切断病毒传播链条、打好非洲猪瘟攻坚战有着重要作用。为摸清四川省宜宾市屠宰企业的非洲猪瘟检测水平,对全市10个区县83家屠宰企业组织开展了非洲猪瘟检测能力比对工作。结果显示:屠宰企业检测人员偏少、岗位工作时间短、文化程度不高;不同区县、不同类别、不同组合的检测结果存在差异,有的差异较大。总的检测敏感性为49.72%,特异性为87.41%,准确率为66.56%。结果表明:屠宰企业的检测能力较为一般,监测风险预警效果不甚理想,有待加强。建议从保障人员队伍和加强操作培训两方面入手,提高屠宰企业非洲猪瘟检测能力。 相似文献
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Jing YUAN Zhitao WANG Pengxiang DUAN Yousheng XIAO Hongke ZHANG Zhixiong HUANG Ruichun ZHOU Hua WEN Kexiong WANG Ding WANG 《Integrative zoology》2021,16(4):499-511
Geographic variations in the dolphin whistles could be useful in assessing association and isolation among populations. Whistle of free-ranging Indo-Pacific humpback dolphins (Sousa chinensis) among the Pearl River Estuary (PRE), Leizhou Bei (LZB) and Sanniang Bay (SNB) populations were investigated. A total of 2850 whistles with legible fundamental contour were extracted and 15 acoustic parameters were measured. Contrary to SNB, PRE and LZB had the same relative proportion of tonal type compositions with flat and sine representing the most frequent types. The generalized linear model analysis showed significant acoustic difference among populations and tonal types. All frequency parameters in SNB were significantly higher than those in PRE and LZB, where no significant variation was observed in most of the parameters either at the population level or within each tonal type. Canonical discriminant functions analysis showed a smaller difference between PRE and LZB than between PRE and SNB and between LZB and SNB. Compared with previous recordings, recent recordings demonstrated a consistent pattern of becoming higher in whistle frequency parameters in both LZB and SNB populations, suggesting that noise pollution in LZB and SNB increasing with time according to the acoustic niche hypothesis. Dolphin whistle's geographic variations could be shaped by the combined function of the geographical barrier function of the Qiongzhou strait and local ambient noise. Considering the isolated condition and the relatively smaller population size of the humpback dolphin in the SNB, more effective and proactive conservation actions should be taken to prevent the extinction of small populations. 相似文献
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应用PAPD方法对鸡败血霉形体DNA多态性的研究 总被引:2,自引:0,他引:2
用随机扩增多态性DNA(RAPD)方法,通过筛选的5个随机引物(OPH-02,OPH-05,OPH-07,OPH-13,OPG-16),对14株鸡败血霉形体(MG)国际标准株和国内分离株进行了DNA多态性研究。结果表明,5个引物共产生21种条带,其中有3个条带分别为2个菌株所特有,扩增产物片段的长度在150~4500bp之间,所有菌株均有1条共同条带,以OPH-05扩增产物的多态性最丰富。根据样品DNA所获得的菌株间相似性指数表明,D9603与D9607相似指数最高,S6,K3913和D9604三者相互间相似性指数最低。提示RAPD方法可用于霉形体遗传标记的分析。 相似文献
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用于检测动物性食品中己烯雌酚残留量的常用方法有放射免疫测定(RIA)、酶免疫测定(EIA)、气相色谱(GC)、高效液相色谱(HPLC)和薄层色谱(TLC)。比较这几种方法,认为RIA法测定的灵敏度较高,测定肝脏中DES的最低检出限可达0.3μg/kg,但对放射防护的要求较高,只能在专门实验室进行。GC法需增加衍生化步骤,检出限为2~10μg/kg。HPLC法用电化学检测器的最低检出限是0.1~0.2μg/kg。TLC法用硅胶作铺板,其最低检出限是0.1μg/kg。EIA法灵敏度高、特异性强,测定中华鳖肌肉中DES的最低检出限0.02μg/kg,是目前用于检测大量样品的首选方法。 相似文献
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The purpose of this study was to detect differential expression of genes related to adipocyte differentiation in pigs by suppression subtractive hybridization. Adipocytes and stromal vascular cells (a fraction containing preadipocytes) from pig adipose tissue were isolated for mRNA extraction. The cDNA from preadipocytes was subtracted from the cDNA from adipocytes. The subtracted gene fragments were cloned into pGEM-T Easy TA cloning vector. We selected 384 clones for gene sequence determination and for further analysis. These genes were subjected to a differential screening procedure to confirm the differential expression of genes between the 2 cell types. We found that at least 36 genes were highly expressed in the adipocytes compared with preadipocytes. Among these, 6 genes including 2 novel genes with the greatest differences were selected and confirmed by Northern analysis. We found that angiotensin I-converting enzyme (ACE), ataxia-telangiectasia mutated protein (ATM), calpain 1, and stearoyl coenzyme A desaturase 1 (SCD1) were highly expressed in adipocytes compared with preadipocytes (P < 0.05). The relative mRNA abundance of ACE, ATM, calpain 1, SCD1, and 2 novel genes discovered in the current study was increased at the later stages of adipocyte differentiation (P < 0.05). The results confirmed that the genes involved in lipid metabolism and adipocyte differentiation were highly expressed in porcine adipocytes. However, further investigation is needed to demonstrate specific functions of the novel genes discovered in the current study. 相似文献
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以安徽白山羊和波尔山羊为试验动物,探讨酵母培养物对其生长发育的影响。结果表明,体重方面,在A组、B组和C组内,试验组比对照组的总增重分别多33.79%、11.76%和18.00%,且A组中试验组总增重和平均日增重极显著高于对照组(P<0.01);体尺方面,A组中试验组体斜长增长显著高于对照组(P<0.05),C组中试验组尻宽增长显著高于对照组(P<0.05),A组中试验组体高增长极显著高于对照组(P<0.01);经济效益方面,A组、B组和C组内的试验组比对照组平均每只羊分别多获利15.87、4.14和8.05元。 相似文献