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581.
This work concerns the pharmacological activity of inuviscolide, a sesquiterpenoid from Inula viscosa. It exerts inhibitory effects on elastase, cyclooxygenase 1 and secretory phospholipase A(2). Furthermore, it reduces the skin leukocyte infiltration in a murine model of dermatitis induced by repeated application of 12-O-tetradecanoylphorbol 13-acetate. 相似文献
582.
Massimo Genovese Concetta Imperatore Marcello Casertano Anna Aiello Francesco Balestri Lucia Piazza Marialuisa Menna Antonella Del Corso Paolo Paoli 《Marine drugs》2021,19(10)
An in-depth study on the inhibitory mechanism on protein tyrosine phosphatase 1B (PTP1B) and aldose reductase (AR) enzymes, including analysis of the insulin signalling pathway, of phosphoeleganin, a marine-derived phosphorylated polyketide, was achieved. Phosphoeleganin was demonstrated to inhibit both enzymes, acting respectively as a pure non-competitive inhibitor of PTP1B and a mixed-type inhibitor of AR. In addition, in silico docking analyses to evaluate the interaction mode of phosphoeleganin with both enzymes were performed. Interestingly, this study showed that phosphoeleganin is the first example of a dual inhibitor polyketide extracted from a marine invertebrate, and it could be used as a versatile scaffold structure for the synthesis of new designed multiple ligands. 相似文献
583.
Elizabeth León-García Gilber Vela-Gutiérrez Oscar A. Del Ángel-Coronel Cristobal Torres-Palacios Javier De La Cruz-Medina Miguel A. Gómez-Lim Hugo Sergio García 《Plant foods for human nutrition (Dordrecht, Netherlands)》2017,72(4):380-387
A healthy lifestyle includes fruits and vegetables consumption. Tomato is one of the most consumed vegetables, although it is susceptible to physical damage through postharvest handling, thus leading to important losses. Softening is an important variable during tomato ripening; excessive softening is undesirable and leads to postharvest losses. TomloxB plays an important role in ripening, mainly in the loss of cellular integrity caused by fatty acids released from the lipid matrix of membranes that initiate oxidative deterioration, which is in turn carried into senescence. In order to increase postharvest life, we produced transgenic tomato plants via Rhizobium radiobacter with tomato lipoxygenase B (TomloxB) antisense constructs under control of the cauliflower mosaic virus (CaMV) 35S promoter. Lipoxygenase activity and firmness were measured in tomato fruit and the fatty acids profile was determined. Transgenic fruits were maintained for 40 days at room temperature in optimal conditions, whereas wild type fruits remained in similar conditions for only six days. Firmness in pink and red stages was significantly lower in wild type fruits than in two transgenic lines. Linolenic acid was the most important fatty acid consumed by lipoxygenase in both turning and pink stages of ripening. Lipoxygenase activity was smaller in transformed fruits in comparison with the wild type. These results suggest that silencing the TomloxB gene promoted significant changes in the physiology of transformed tomatoes, being the increase in postharvest life the most important. 相似文献
584.
585.
The mechanisms behind the pathogenesis of rainbow trout gastroenteritis (RTGE) are still unknown. This study examined the macroscopic and microscopic changes in trout with RTGE (RTGE+), as well as the blood chemistry. A total of 464 rainbow trout were sampled from 11 sites in the UK, comprising 152 RTGE+ fish and 330 random, apparently healthy fish. A case definition for RTGE was assessed by the analysis of its agreement with three laboratory tests: histopathology, packed cell volume and kidney bacteriology. Cluster analysis indicated the presence of three distinct presentations within the population of RTGE+ fish. Cluster A included gross signs associated with moribund RTGE+ fish, and clusters B and C identified gross signs consistent with concurrent diseases, notably furunculosis, enteric redmouth and proliferative kidney disease. The information gained was used to select RTGE+ fish without concurrent disease for the analysis of RTGE pathogenesis with blood biochemistry. This analysis revealed a severe osmotic imbalance and a reduced albumin/globulin ratio as indicatives of selective loss of albumin. These findings are compatible with a protein losing enteropathy. 相似文献
586.
Juan M. Pacheco‐Vega M. Del Pilar Sánchez‐Saavedra 《Journal of the World Aquaculture Society》2009,40(4):556-560
The aim of this work was to demonstrate that the source of nitrogen of microalgae growth media has an effect on the biochemical composition by comparing the composition of Chaetoceros muelleri grown in semicontinuous cultures with 30% daily dilutions with an agricultural fertilizer containing three nitrogen sources (AF) and with the traditional f/2 medium. The cell yields, proximate composition, and total essential and nonessential amino acid contents of C. muelleri were similar, but phenylalanine and glutamate were significantly higher with f/2, and alanine was higher with AF. Monounsaturated fatty acids were higher in AF‐grown cultures, but polyunsaturates were higher with f/2. Among polyunsaturates, 20:5n‐3 (eicosapentaenoic acid, EPA) was higher with AF (17.16%) than with f/2 (13.44%), whereas f/2 yielded cells richer in 20:4n‐6 (arachidonic acid, ARA: 2.08% vs. 1.38%) and 22:6n‐3 (docosahexaenoic acid, DHA: 6.86% vs. 3.80%). Our results provide evidence that different nitrogen sources may cause variations in the biochemical profile of C. muelleri. 相似文献
587.
Castillo J Benavente-García O Lorente J Alcaraz M Redondo A Ortuño A Del Rio JA 《Journal of agricultural and food chemistry》2000,48(5):1738-1745
The quantitative distribution of several flavan-3-ols was determined using HPLC in a grape (Vitis vinifera) seed extract (GSE) of four cultivars grown in the region of Murcia. Polymer >/= C(4) units made up the largest group of procyanidins in the GSE (90.92%, expressed as HPLC % area). The antioxidant activity of GSE and other reference compounds was investigated by measuring their ability to scavenge the ABTS(*)(+) radical cation (TEAC). The most effective compounds were, in order: GSE > rutin > (+)-catechin > diosmin >/= ascorbic acid. The radioprotective effects of GSE and other reference compounds were determined by using the micronucleus test for anticlastogenic activity, any reduction of the frequency of micronucleated polychromatic erythrocytes (MnPCEs) being evaluated in the bone marrow of mouse exposed to X-rays. The most effective compounds were, in order: GSE > rutin > dimethyl sulfoxide (DMSO) > ascorbic acid > 6-n-propyl-2-thiouracil-6c (PTU) > diosmin. The higher ABTS(*)(+) scavenging capacity and anticlastogenic activity of GSE can be explained, structurally, by the high number of conjugated structures between the catechol groups in the B-rings and the 3-OH free groups of the polymeric polyphenolic skeleton and, in addition, by the stability of the aroxyl flavonoid radical generated in the above processes. 相似文献
588.
Costantino Vischetti Mario Businelli Mauro Marini Ettore Capri Marco Trevisan Attilio A. M. Del Re Lucia Donnarumma Elisa Conte Giancarlo Imbroglini 《Pest management science》1997,50(3):175-182
Experiments were carried out on three Italian farms to assess the degree of spatial variation of pesticide field concentration during treatment and during dissipation trials. Test pesticides were chloridazon and metamitron (both sugar-beet herbicides) applied as a tank mix. The classical statistical technique and geostatistics were used to summarize and evaluate variable spatial data. The results show that the actual values of pesticide concentration for application rate and initial concentration in all three areas are lower than expected, thus indicating that under field conditions only a part of the pesticide reaches the soil during the distribution. The actual values for both herbicides in all three areas expressed as percentage of expected values ranged from 44·1% to 64·2% for application rate and from 40·5% to 99·5% for initial concentration. The coefficient of variation was similar for both pesticides and ranged from 23·8 to 74·1 for application rate, 24·1 and 58·8 for initial concentration and 11·1 and 110·0 for dissipation half-lives. The high variability in application rate and initial concentration could be ascribed to an uneven herbicide distribution, and in dissipation studies to variation in half-lives for the rate of herbicide loss from soil in different parts of the field. Geostatistic analysis indicated little spatial correlation, probably because the sampling sites were widely spaced on the field. In all cases, the data were not sufficient to estimate the range of influence, probably because of the size of the experimental fields and the sampling strategy. © 1997 SCI. 相似文献
589.
Fernando Sicbaldi Gian Attilio Sacchi Marco Trevisan Attilio A. M. Del Re 《Pest management science》1997,50(2):111-119
A pressure-chamber technique was used to study the root uptake and xylem translocation of some fungicides, herbicides and an insecticide from different chemical classes in detopped soybean roots. Physiological parameters such as K+ leakage from roots, K+ concentrations in the xylem sap, and protein and ATP levels in the root cells were measured so as to evaluate any potential damage of this technique to the root system. HPLC was used to quantify the compounds in the xylem sap. The pressure-chamber technique has proved useful to study the root uptake and translocation of pesticides, does not damage the root system, and allows one to obtain appreciable volumes of xylem sap that can be analysed directly by HPLC, thus avoiding dependence on the availability of radio-labelled compounds. The concentration of each pesticide in the xylem sap showed a steady-state kinetic profile. Non-linear regression analysis was used to calculate the steady-state concentration and the time required to achieve 50% of the steady-state concentration (TSSC50). TSSC50 was well correlated with log Kow; the more lipophilic the compound the more time was required to reach the steady-state concentration. The efficiency of translocation was assessed by the transpiration stream concentration factor (TSCF) and a non-linear relationship between TSCF and log Kow was observed. The highest TSCF values were measured for those compounds with log Kow values around 3, a lipophilicity value similar to that reported earlier in an analogous experiment with detopped soybean plants but slightly higher than that reported in earlier experiments with intact barley plants. Lower TSCF values were obtained with chemicals with log Kow values below as well as above 3. © 1997 SCI. 相似文献
590.
J. Casalí J. Díez J. Del Valle de Lersundi M. Goñi Y. Chahor J. López 《Agricultural Water Management》2010,97(10):1683-1694
An experimental watershed (Oskotz principal- Op -ca.1700 ha) covered with forest and pasture (cattle-breeding) with an equally experimental sub-watershed (Oskotz woodland - Ow - ca. 500 ha) almost entirely under forest was continuously monitored during 8 years (2001-2008). These watersheds were established by the Government of Navarre (Spain) in order to assess the impact of agricultural activities on different region of Navarre. The first results regarding exported sediment, runoff, nitrate and phosphate are presented herein. These results are compared with those from two grain-sown watersheds previously reported by the authors, elsewhere.The same as in the grain-sown watersheds, most runoff, sediment, nitrate and phosphate yields in Oskotz were generated during winter, though most erosive rainfalls occurred during summer. In Ow, average sediment, nitrate and phosphate yields were approximately: 700, 22, 0.35 kg ha year−1, respectively; for Op these figures were 550, 54 and 0.76 kg ha year−1, respectively.However, total sediment and solute yields were different depending on the prevailing land use: cereal crops > forest > pasture. Sediment yields in the forest were strongly affected by the logging moment, when exported sediment rocketed.Nitrate concentration and yields were lower (and under the critical threshold) in the forested/pastured watersheds than those recorded in the two intensively cultivated watersheds. However, phosphate yields were dramatically higher (and over the critical threshold) in the former watersheds due to the prevailing soil conditions and to the fertilization of pasture, mainly with slurry.The present work, along with that similar one recently reported by the authors, is an unprecedented and relevant piece of research for the region. 相似文献